Benchmark Scientific Accuris, Instruction Manual, Page: 15 / 60

15
General Operation
Sample Preparation
Required materials: Cell suspension, 0.4% trypan blue, micro tube 1.5ml, pipette, tips, and
QuadSlides™. Preparation should be done in a clean area to avoid dust contamination (dust on the
slides or in the samples will greatly reduce counting accuracy).
STEP 1. Prepare necessary items.
STEP 2. Place 20 μL of trypan blue in the micro tube and add an equal volume of the cell
suspension.
NOTE: Before sampling the cell suspension, gently resuspend the cells at least 6 times
(pay attention to avoid bubbles and check if there are any cell clumps or agglomerates)
The sampling should be in middle of the cell suspension, not on the surface or the bottom.
STEP 3. Mix the sample in the micro tube by pipetting the vial 3~5 times gently.
NOTE: Be careful not to create bubbles.
STEP 4. Load 20 μL of the stained cell sample into each channel of a QuadSlide™.
NOTE: The samples should be from the middle of the cell suspension, not from the surface or the
bottom, and ensure that no bubbles enter the slide channesl.