Beckman Coulter Navios, Instructions For Use Manual

Page: 245 / 586

773232AH
10-49
Using Navios Software
File Menu
10
Baseline Offset
CAUTION
Risk of erroneous results. The Baseline Offset function should only be used after
first viewing data with the baseline offset function turned off (unchecked). You
must be satisfied that the overall results of any assay are not significantly
affected by turning baseline offset on. If the lower marker of a statistics region is
higher than the first decade (of log displayed data), then there should be no effect
on statistics from that region by using baseline offset. However, you should not
use baseline offset when determining appropriate cytometer settings as with
AutoSetup. Baseline offset On should only be used for visual purposes after
analysis.
This function allows log data in very low level signals to be moved up from low numbered channels
by adding a randomized Gaussian positive offset. This allows easier comparison of data from other
Cytometers. Baseline Offset includes the following three options:
•
Auto
This option displays the listmode data according to a keyword in the FCS file header. If the
Baseline Offset keyword value is ON, then data is automatically displayed with Baseline Offset
switched on. If it is OFF, then data is displayed with no Baseline Offset.
•
On
This reads a file and modifies the displayed data only by adding a randomized Gaussian offset to
the low-level signals. Data on disk is not modified and is left as it was when acquired from the
Cytometer.
•
Off
Reads a file exactly as it is with no Baseline Offset. This setting ignores the runtime setting of
Baseline Offset and always displays data without the Baseline Offset effect.
NOTE
Changing Baseline Offset settings on the Cytometer Control dialog does not affect the currently
loaded listmode file. Only changes made on the Workspace Preferences dialog affect the currently loaded
listmode file. If the desired Baseline Offset option is already selected in Workspace Preferences, select a
different Baseline Offset option, select , and then reselect the desired Baseline Offset
option again.
If Workspace Preferences already has the desired option selected, select another option, and
then select the desired option.
Default Plot Size
Allows you to select the size of the plot display for newly created plots.
NOTE
To change the size of plots already present on the workspace,
Window
>
Tile Special
and select
the desired plot size to update the size of the existing plots displayed on the workspace.