Beckman Coulter Navios, Instructions For Use Manual, Page: 157 / 586

773232AH
7-3
Quality Control
Daily QC
7
Daily QC
Daily QC consists of:
•
Preparing AutoSetup Samples
•
Running the AutoSetup Scheduler
•
Running the AutoSetup II Wizard
CAUTION
Risk of erroneous results if the Cytometer has been idle for an extended period of
time or you have just performed Daily Startup. To ensure correct results, perform
a prime after:
• Daily Startup.
• The Cytometer has been idle for an extended period of time.
• You place a new carousel on the MCL and light scatter signals appear
abnormal.
Preparing AutoSetup Samples
1
Prepare the Flow-Check Pro fluorospheres Follow the package insert instructions for mixing
and handling the fluorospheres.
2
Prepare the Flow-Set Pro fluorospheres. The Flow-Set Pro fluorospheres tube is used to set the
detector gains and voltages to the required level. Follow the package insert instructions for
mixing and handling the fluorospheres.
QC Process QC Material Used
Verify fluidics and laser
alignment Flow-Check Pro Fluorospheres. Verify HPCV versus expected value. Export
results to the QC Database and review the QC data.
Adjust high voltage and
gain for a given application Flow-Set Pro Fluorospheres. Ascertain target mean position based upon
application and adjust high voltage and gain daily to that target.
Export
results to the QC Database and review the QC data .
Perform absolute counts Flow-Count Fluorospheres.
Adjust color compensation
for a given application For AutoSetup applications, Cyto-Comp Cells or whole blood stained with
QuickCOMP 2 or QuickCOMP 4 kit. Use single color stained samples with
each fluorochrome used in your application.
Verify correct settings with
an application Control Update the control protocol with the settings derived from above. Run a
biological control equivalent to the application, such as Immuno-Trol Cells,
Immuno-Trol Low Cells, Cyto-trol Control Cells, or a normal whole blood.
Export results to the QC Database and review the QC data.