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Beckman Coulter JA-14.50, Instructions For Use Manual

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Summary of Contents for JA-14.50

Page 1: ...PN B32164AC October 2016 Instructions For Use JA 14 50 Fixed Angle Rotor For Use in Beckman Coulter Avanti J Series Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A...

Page 2: ...e United States and other countries All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders Contact Us If you have any questions con...

Page 3: ...em help for your instrument Issue AC 07 2016 Updates were made to the following sections Safety Information for the JA 14 50 Rotor Specifications Description Preparation and Use Tubes Replacement Roto...

Page 4: ...PN B32164AC iv Revision History...

Page 5: ...any situation contact your Beckman Coulter Representative Alerts for Warning Caution and Note WARNING WARNING indicates a potentially hazardous situation which if not avoided could result in death or...

Page 6: ...on The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors or a hazardous chemical reaction Do not centrifuge such materials in nor handle...

Page 7: ...figurations 8 Rotor Installation 9 Run Procedure 10 Removal and Sample Recovery 10 Dual Locking Lid 10 Single Locking Lid 11 If the Rotor Sticks to the Drive Hub 12 Tubes 13 Tubes for JA 14 50 Rotors...

Page 8: ...viii Contents Beckman Coulter Inc J Series Rotor Warranty...

Page 9: ...s in the Rotor 5 4 Rotor Drive Pin Location and Orientation 7 5 Centrifuge Drive Hub Configurations 7 6 Installing the Rotor 8 7 Using the Rotor Removal Tool 12 8 Rotor Serial Number with T 13 9 Rotor...

Page 10: ...er Bottles and Tubes for the JA 14 50 Rotor manufactured prior to October 2016 14 3 Adapters to support tubes from other manufacturers for the JA 14 50 Rotor manufactured after October 2016 16 4 Avail...

Page 11: ...l tube capacity 50 mL Nominal rotor capacity 800 mL Approximate acceleration time to maximum speed rotor fully loaded 4 min Approximate deceleration time from maximum speed rotor fully loaded max brea...

Page 12: ...was tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed The rotor is al...

Page 13: ...ge drivehub to preventtherotor from slippingduringacceleration and deceleration JA 14 50 rotors manufactured after October 2016 have a slightly tapered cell profile which improves performance of most...

Page 14: ...cks Read the Safety Notice section at the front of this manual before using the rotor 1 Make sure that the rotor lid and all tubes or accessories are clean and show no signs of corrosion or cracking 2...

Page 15: ...PN B32164AC 5 JA 14 50 Fixed Angle Rotor Preparation and Use Figure 3 Typical Examples of Arranging Sixteen or Fewer Tubes in the Rotor...

Page 16: ...Speeds for information about speed limitations Installing the Rotor Rotor Drive Pin Orientation Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive hub to prevent the...

Page 17: ...rive Spindle Hub 1 Drive Hub Grooves 4 2 Rotor Pins 2 3 Drive Hub 4 Drive Spindle Assembly 5 Older Model Centrifuges Be sure the pins in the rotor drive hole are seated in the grooves of the drive spi...

Page 18: ...s the lid to be attached to and removed from the rotor outside the centrifuge so that tubes can be loaded and unloaded under a safety hood The dual locking lid consists of a daisy knob that attaches t...

Page 19: ...ight clockwise until secure Do not overtighten 4 Turn the rotor until the Beckman Coulter name or the engraved drive pin alignment indicators are either horizontal or perpendicular to the drive hub pi...

Page 20: ...rotor is properly loaded and installed in the centrifuge you are ready to perform the run Refer to the applicable centrifuge instruction manual for operating instructions WARNING Operator error or tu...

Page 21: ...or place your hands on the rotor and lift the rotor straight up off the drive hub 3 Place the rotor on a counter or bench top under a safety hood 4 Grasp the daisy knob and turn it to the left counter...

Page 22: ...roken when the lid is removed Consult your laboratory safety officer for the proper safety procedures to follow in this case 1 Remove the lid 2 Screw the removal tool into the threaded opening As the...

Page 23: ...nformation on the chemical compatibilities of tube and accessory materials Tubes for JA 14 50 Rotors Manufactured before October 2016 CAUTION The tubes listed in Table 1 should be used one time only T...

Page 24: ...ufactured before October 2016 Type and Volume Tube Description Required Adapter Effective rmax mm Effective rmin mm Max Speed RCF k Factor Table 2 Available Beckman Coulter Bottles and Tubes for the J...

Page 25: ...034 1 14 000 RPM 33 400 g 665 18 100 mm 15 mL Polyethylene Round Bottom open top tube 342082 15 B40157 Qty 2 ea B30034 1 14 00 RPM 33 400 g 665 16 80 mm 10 mL Polycarbonate Round Bottom bottle with ca...

Page 26: ...onical 50 mL Polypropylene conical tube with capbcdef N A 159 7 98 8 g conical 15 mL Polypropylene conical tube with capbcef B89429 Qty 2 ea B19962 156 2 92 2 g conical 5 mL Polypropylene conical tube...

Page 27: ...ize the possibility of tube failure observe the following conditions Do not use tubes more than once Discard crazed tubes Refer to Table 5 to find the g forces produced at various speeds in the rotor...

Page 28: ...radius and rmin is the minimum radius After substitution EQ 2 In the JA 14 50 rotor the k factor is 787 for full 50 mL tubes at maximum speed For containers that nominally hold less than 50 mL the k f...

Page 29: ...adjusting the k factor as follows EQ 5 Run times can also be estimated from data established in prior experiments using a different rotor if the k factor of the previous rotor is known For any two ro...

Page 30: ...solutions more dense than 1 2 g mL are centrifuged in this rotor use EQ 7 to calculate the reduced maximum allowable rotor speed EQ 7 Table 5 Relative Centrifugal Fields for the JA 14 50 Rotora a Ent...

Page 31: ...r surface Corrosion begins in scratches and may open fissures in the rotor with continued use 1 Periodically at least monthly inspect the rotor especially inside cavities for rough spots or pitting wh...

Page 32: ...apply silicone vacuum grease to the O rings Replace O rings about twice a year or whenever worn or damaged 5 Refer to Chemical Resistances publication IN 175 for the chemical compatibilities of rotor...

Page 33: ...ater 4 Air dry the rotor and lid upside down Do not use acetone to dry the rotor 5 Apply a thin even coat of silicone vacuum grease to both lid O rings before replacing them in the lid 6 Clean metal t...

Page 34: ...and lid in the autoclave upside down Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discolor...

Page 35: ...a sealed plastic bag All parts must be accompanied by a note plainly visible on the outside of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or...

Page 36: ...n go to the Beckman Coulter website at www beckmancoulter com Description Part Number Tubes and adapters see Table 1 Table 2 Table 3 and Table 4 Rotor removal tool 360998 Rotor Cleaning Kit 339558 Sil...

Page 37: ...dit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Indianapolis Indiana facility or...

Page 38: ...PN B32164AC Warranty 2 Beckman Coulter Inc J Series Rotor Warranty...

Page 39: ......

Page 40: ...ailable in electronic pdf or CD ROM by request Rotors and Tubes CD ROM 369668 Rotors and Tubes for Tabletop Preparative Ultracentrifuges Rotors and Tubes for J2 J6 Avanti J Series Centrifuges Rotors a...

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