Beckman Coulter JA-14.50, Instructions For Use Manual

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PN B32164AC
17
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Temperature Limits
•
Plastic tubes have been centrifuge tested for use at temperatures between 2 and 25°C. For
centrifugation at other temperatures, pretest tubes under anticipated run conditions.
•
If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior
to centrifugation.
The JA-14.50 rotor generates
g
-forces higher than the tube manufacturers’ recommended
maximums (see Table 1 , Table 2 , Table 3 , and Table 4 ). Running tubes above the recommended
maximum
g
-forces will cause white striations known as crazing (see Figure 10 ). Moderate crazing
will not affect run results or cause tube leakage. However, repeated tube usage and/or long run
times will eventually lead to tube failure. To minimize the possibility of tube failure, observe the
following conditions.
•
Do not use tubes more than once.
•
Discard crazed tubes.
•
Refer to Table 5 to find the g -forces produced at various speeds in the rotor, and limit run speed
to the speed that produces the maximum recommended
g
-force for the tube in use.
Table 4
Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotor
a
manufactured after October 2016
Nominal
Dimensions
and Volume
Tube Description
Part
Number
Nominal
Volume
(mL)
Required
Adapter
P/N
Tubes
per
Adapter
Max
Speed/RCF/
k Factor
18

100 mm
15 mL
Polycarbonate Round Bottom
open-top tube 342080 15 B89430
(Qty 2 ea
B19963)
1 14,000 RPM
33,400

g
665
18  100 mm
15 mL
Polypropylene Round Bottom
open-top tube 342081 15 B89430
(Qty 2 ea
B19963)
1 14,000 RPM
33,400 
g
665
18  100 mm
15 mL
Polyethylene Round Bottom
open-top tube 342082 15 B89430
(Qty 2 ea
B19963)
1 14,000 RPM
33,400 
g
665
a. Use only the items listed here and observe fill volumes and maximum run speeds.
2°C