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Beckman Coulter J-Lite JLA 16.250, Instruction Manual

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Beckman Coulter J-Lite JLA 16.250 Instruction Manual Download Page 10

10

J-Lite JLA-16.250 Rotor

5. Once the rotor is properly aligned carefully lower it straight down 

onto the hub. 

Do not drop the rotor onto the hub

.

6. Slowly turn the rotor around the drive spindle to make sure that it 

is properly seated. In rare cases, the rotor pins can rest on top of 
the spindle hub pins or teeth, which prevents the rotor from being 
secured to the drive spindle. Turning the rotor after installing it 
will move the pins into proper position.

Figure 3. Rotor Top View: Indicator Marks Show Drive Pin Orientation

7. Secure the rotor to the centrifuge drive spindle as follows:

Dual-locking lid

: press the lid knob down and turn it to the right 

(clockwise) until secure, no more than two full turns. Do not 
overtighten.

Single-locking lid

: place filled tubes into the rotor, then place the 

lid on the rotor. Press the lid knob down and turn it to the right 
(clockwise) until secure. Do not overtighten.

WARNING

!

If the rotor is left in the centrifuge between 
runs, make sure that the rotor is seated on the 
drive hub and that the tie-down knob is tight 
before each run.

Tie-down Knob

Knob Threads

Single-Locking Lid

Indicator Marks

Summary of Contents for J-Lite JLA 16.250

Page 1: ...J Lite JLA 16 250 Fixed Angle Rotor Used In Beckman Coulter J2 and Avanti J Series Centrifuges J TB 072JB June 2018 2018 Beckman Coulter Inc ...

Page 2: ...man Coulter prod uct and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter Inc in the United States and other countries All other trademarks service marks products or services are trade marks or registered trademarks of their respective holders ...

Page 3: ...ent Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materi...

Page 4: ...ax 134 mm 38 400 g at rav 90 mm 25 800 g at rmin 46 mm 13 200 g k factor at max speed in Avanti J series centrifuges 1090 k factor at max speed in J2 series centrifuges 1400 Conditions requiring speed reduction see RUN SPEEDS Maximum allowable imbalance of opposing loads 10 grams Number of bottle cavities 6 Available bottles and tubes see Table 3 Tube angle 25 Nominal tube capacity of largest tube...

Page 5: ... filled tubes may also be used to purify large quantities of virus in a cushion gradient The rotor and lid are made of aluminum and are anodized for corro sion resistance the rotor is black and the lid is blue The tie down knob in the rotor lid secures the rotor to the centrifuge drive hub O rings made of Buna N rubber located in the rotor lid help to main tain atmospheric pressure inside the roto...

Page 6: ...rotor is warranted for 7 years see the WARRANTY PREPARATION AND USE Specific information about the JLA 16 250 rotor is given here Use the Rotors and Tubes for J Series Centrifuges manual publication JR IM and the centrifuge instruction manual along with this manual for complete rotor and accessory information NOTE Although rotor components and accessories made by other manufacturers may fit in the...

Page 7: ...an room temperature refrigerate or warm the rotor before the run for fast equilibration 1 Be sure that the metal threads in the rotor are clean and lightly lubricated with Spinkote lubricant 306812 Also ensure that lid O rings are lightly but evenly coated with silicone vacuum grease 335148 2 Load filled containers symmetrically into the rotor Refer to BOTTLES AND TUBES on page 15 for information ...

Page 8: ... IN A MICROPROCESSOR CONTROLLED J2 SERIES CENTRIFUGE below if you are using the rotor for the first time BEFORE USING THE JLA l6 250 ROTOR IN A MICROPROCESSOR CONTROLLED J2 SERIES CENTRIFUGE If you have a microprocessor controlled Beckman Coulter J2 series centrifuge model J2 MI J2 21M J2 21M E or J2 MC you will need to enter the JLA 16 250 rotor identification code 14 and maximum speed l4 000 rpm...

Page 9: ... program can only be logged into memory from level 3 and only when all three entries are complete Once this procedure is complete the microprocessor memory will retain the information and it will not have to be entered again Each time you run the JLA 16 250 rotor enter code 14 as described in the centrifuge instruction manual INSTALLING THE ROTOR 1 To prevent the rotor from sticking to the centrif...

Page 10: ...e 3 Rotor Top View Indicator Marks Show Drive Pin Orientation 7 Secure the rotor to the centrifuge drive spindle as follows Dual locking lid press the lid knob down and turn it to the right clockwise until secure no more than two full turns Do not overtighten Single locking lid place filled tubes into the rotor then place the lid on the rotor Press the lid knob down and turn it to the right clockw...

Page 11: ...owing temperature offset table to set the desired sample temperature Table 1 Temperature Settings for Model J E High Performance Centrifuge At speeds above 13 000 rpm use the indicated temperature setting for the desired sample temperature Interpolate if intermediate values are required At some speed and temperature combinations the centrifuge may be unable to achieve the desired sample temperatur...

Page 12: ...r the Model J2 HC Analog Centrifuge Interpolate if intermediate values are required N indicates that the rotor cannot achieve the required temperature at this speed Analog J2 Series Centrifuges Models J2 HS J2 21 and J2 HC 1 Turn the SET knob on the centrifuge control panel to the required sample temperature 2 Find the compensation value in Table 1 or Table 2 that corre sponds to the required temp...

Page 13: ...isplay and the display flashes 4 Use the keypad to enter the desired compensation value Press the key to enter a minus sign pressing it again will remove the minus sign 5 Check the temperature display If the entry is incorrect press CE and reenter the digits 6 When the entry is correct press ENTER RECALL NOTE To clear a COMP ADJ entry press COMP ADJ 0 and ENTER RECALL RUN PROCEDURE WARNING Operato...

Page 14: ...LE RECOVERY CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories as required Dual Locking Lid 1 Place one hand on the rotor to keep it from turning Grasp the tie down knob with your other hand and turn it to the left counter clockwise while pulling up until the stem dise...

Page 15: ... hub Single Locking Lid 1 Unscrew the tie down knob to release the rotor from the drive hub 2 Remove the lid and set it aside 3 Lift the rotor straight up off the drive hub Do not lift the rotor by the tie down knob NOTE Lubricating the centrifuge drive hub with Spinkote should prevent the rotor from sticking in J2 series centrifuges In Avanti J series centri fuges the drive hubs do not need lubri...

Page 16: ... the run Refer to Appendix A in Rotors and Tubes for information on the chem ical resistances of tube and accessory materials Temperature Limits Plastic containers have been tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest containers under anticipated run conditions If plastic containers are frozen before use make sure that they are thawed to at le...

Page 17: ...es per Adapter 62 120 mm 250 mL polypropylene bottle w cap assembly 356011 250 none 16 000 rpm 38 500 g 1 070 62 120 mm 250 mL polycarbonate bottle w cap assembly 356013 250 none 16 000 rpm 38 500 g 1 090 29 104 mm 50 mL polypropylene bottle w cap assembly 357001 40 adapter 356997b 1 16 000 rpm 32 200 g 650 29 104 mm 50 mL polycarbonate bottle w cap assembly 357000 40 adapter 356997 1 16 000 rpm 3...

Page 18: ... not less than half full Again all opposing containers for a run must be filled to the same level RUN TIMES The k factor is a measure of a rotor s pelleting efficiency Beckman Coulter has calculated the k factor for all of its rotors at maximum speed with full bottles or tubes using the following formula 1 Where ω is the angular velocity of the rotor in radians per second ω 0 105 rpm rmax is the m...

Page 19: ...n on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 RUN SPEEDS The centrifugal force at a given radius in a rotor is a function of run speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rotational speed is adjusted so that identical samples are subjected to ...

Page 20: ...29 400 25 400 21 600 25 800 22 700 19 800 17 000 14 500 13 200 11 600 10 100 8 710 7 420 11 000 10 000 9 000 8 000 18 200 15 000 12 200 9 610 12 200 10 100 8 160 6 450 6 230 5 150 4 170 3 300 7 000 6 000 5 000 4 000 7 350 5 400 3 750 2 400 4 940 3 630 2 520 1 610 2 520 1 860 1 290 824 3 000 2 000 1 000 500 1 350 600 150 38 907 403 100 25 464 206 52 13 0 5 000 10 000 15 000 20 000 25 000 30 000 35 ...

Page 21: ... two figures CARE AND MAINTENANCE INSPECTION Periodically at least monthly inspect the rotor body for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not use the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program FRIP and the rotor repair center Before using th...

Page 22: ... Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues If the r...

Page 23: ...he O ring grooves with a cotton tipped swab Reapply a light film of silicone vacuum grease DECONTAMINATION If the rotor and or accessories becomes contaminated with radioac tive material decontaminate it using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found several that do not harm anodized aluminum RadCon Surface Spray or IsoClean S...

Page 24: ...6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of sterility or disinfec tion is expressed or implied When ster...

Page 25: ...n receipt of the rotor or accessory at the factory and name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a s...

Page 26: ...ons referenced in this document can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office REPLACEMENT ROTOR PARTS JLA 16 250 rotor assembly biosafe with dual locking lid 363930 JLA 16 250 rotor assembly with single locking lid 363934 Dual locking lid assembly 363931 Single locking lid assembly 363935 Large lid O ring qty 2 ...

Page 27: ...27 J Lite JLA 16 250 Rotor ...

Page 28: ...28 J Lite JLA 16 250 Rotor ...

Page 29: ... of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Indianapolis Indiana facility or delivered to a Beckman Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows Replacement price Current rotor list price CONDITIONS 1 Except as otherwise sp...

Page 30: ...Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 U S A Sales and Service 1 800 742 2345 Internet www beckman com Printed in U S A 2018 Beckman Coulter Inc All rights reserved ...

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