Beckman Coulter CEQ 8000, User Manual

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Exporting Database Items
User’s Guide 317
Data Manager Export File Types
Listed in Table 74 are the available export file types.
Table 74: Data Manager Export Options
Format Description
SCF (.scf)
The SCF (Standard Chromatogram Format) format is used to store analyzed
DNA sequence or fragment data for a single sample. The format describes both
“public” and “private” data sections. The well-defined public data section
contains trace data points, the called sequence or identified fragments and the
positions of each called base or fragment within the trace data. A public comment
section is also defined. The “private” section can be used to store other
information that will generally be ignored by most software packages.
The CEQ System uses the private data section to store sample data (including raw
data along with current and voltage data). Sample data from either sequencing or
fragment analysis samples can be stored. The notes and property sets are stored
in the comment section. The CEQ System software supports SCF versions 2.1
and 3.0. (You would use this format, for instance, to export analyzed sequence
data into Lasergene’s SeqMan from DNASTAR Inc.). Third-Party packages
cannot currently view raw data, current, or voltage. However, custom
applications can be written to view the data.
Use the SCF format to transfer data from instrument to instrument, or to import
data from third-party software into the CEQ Sequence Analysis module. If you
use SCF to exchange data between instruments you will lose informational data
such as sample plate name, capillary array serial number, etc. If you wish to
maintain exact copies of data including header information, use the CEQ format
and import and export these data via the Data Manager.
Tab-
Delimited
Text (.txt)
The Tab-delimited format is valid for raw data, sequence results and fragment
results. Check the Raw Data check box to include raw data, as well as current and
voltage data. Check the Results Data and Results Output check boxes to include
the sequence results.
The data points will be expressed as text values and can be viewed in any text
editor or in Excel.
SEQ (.seq)
The SEQ format only applies to the sequence results portion (e.g., text bases) of
analyzed data. You would use this format, for instance, to export data into
Lasergene’s SeqMan from DNASTAR Inc.