Beckman Coulter Biomek 3000 Page 1 Quick Start Manual Download

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Beckman Coulter, Inc

4300 N. Harbor Boulevard, Fullerton, CA 92834-3100

Biomek

3000 Laboratory

Automation Workstation

Quick-Start Guide

Beckman Coulter PN 987837

Revision AB

September 2004

Summary of Contents for Biomek 3000

Page 1: ...c 4300 N Harbor Boulevard Fullerton CA 92834 3100 Copyright 2004 Beckman Coulter Inc Printed in U S A Biomek 3000 Laboratory Automation Workstation Quick Start Guide Beckman Coulter PN 987837 Revision...

Page 2: ...of this system and related documentation or the procedures specified in this manual regardless of foreseeability or the form of action whether in contract tort including negligence breach of warranty...

Page 3: ...product claimed to be defective must if required by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with transportation charges collect unless t...

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Page 5: ...tioners 1 4 1 2 5 Tool Racks 1 4 1 2 6 Optional Devices 1 5 1 3 Biomek Software 1 6 1 4 Starting the Biomek 3000 1 7 1 4 1 Turning On the Biomek 3000 1 7 1 4 2 Launching the Software 1 7 1 4 3 Homing...

Page 6: ...Creating Methods 3 1 Overview 3 1 3 2 General Description of Editors 3 2 3 2 1 Project Menu 3 3 3 2 2 Instrument Menu 3 3 3 3 Creating New Labware in the Labware Type Editor 3 5 3 4 Creating a New Pa...

Page 7: ...his chapter a general hardware overview on the Biomek 3000 Automated Laboratory Workstation and introduction to the Biomek Software Learning to Create Methods for the Biomek 3000 Chapter 2 an introduc...

Page 8: ...en instructions on creating a small method refer to Chapter 2 Learning to Create Methods for the Biomek 3000 and utilizing the editors refer to Chapter 3 Using the Editors While Creating Methods Some...

Page 9: ...ate washing operations and moving labware around the deck The modular design of the Biomek 3000 allows expansion of the instrument capabilities to include additional operations such as filtration plat...

Page 10: ...Biomek 3000 deck refer to the Biomek 3000 Laboratory Automation Workstation User s Manual Chapter 3 Gripper Tool wash system the wash system includes single channel and eight channel tools used to pe...

Page 11: ...to the Biomek 3000 Laboratory Automation Workstation User s Manual Chapter 9 Thermal Exchange Unit Filtration System includes a vacuum valve unit that is connected to the Biomek 3000 workstation via...

Page 12: ...er to Chapter 2 Learning to Create Methods for the Biomek 3000 When a step in the Method View is highlighted the corresponding configuration options appear in the Step Configuration Area refer to Chap...

Page 13: ...g into Biomek Software for the first time you must enter a new password Remember your new password you will need to enter it each time you access Biomek Software Figure 1 2 Biomek Software main editor...

Page 14: ...ed To home all axes 1 From the Instrument menu choose Home All Axes A Warning appears 2 Make sure that all warnings are addressed and choose OK The pod is homed and the instrument is now ready for use...

Page 15: ...this chapter and complete the tutorial steps provided After you have completed the instructions or if you need additional information along the way use the links provided to access the suite of user...

Page 16: ...to tell Biomek Software where you have physically placed labware on your deck Before proceeding with this quick start method make sure that an MP200 Multi Tip Pipette Tool and Gripper Tool are added...

Page 17: ...tep in a method provides options to clear the Biomek 3000 deck clear the devices on the deck unload tips from the pod and clear all global variables Refer to the Biomek Software User s Manual Chapter...

Page 18: ...software what labware and devices are on the deck for obstacle avoidance see sidebar You ll learn more about the Deck Editor in the next chapter refer to Section 3 7 Creating a New Deck Figure 2 2 Ins...

Page 19: ...Make sure that the latch is securely holding the tip rack in position a Reservoir to P3 a BCFlat96 to P4 Your screen should look similar to Figure 2 3 Note To remove unwanted labware from the Deck La...

Page 20: ...the right side depending on your instrument configuration 4 Drag the Gripper tool to the gripper tool position on the tool rack the larger position on the right 5 Drag the MP200 tool to either of the...

Page 21: ...e default settings in Labware Properties will be used for the tip box however you can refer to the Biomek Software User s Manual Chapter 15 2 5 1 Configuring Labware Properties for Tips to learn more...

Page 22: ...Figure 2 6 Note To learn more about the Instrument Setup step refer to the Biomek Software User s Manual Chapter 15 2 Instrument Setup Step Figure 2 6 Instrument Setup step with labware named Next you...

Page 23: ...d drop a Transfer step from the Step Palette into the Method View just below the Instrument Setup step Notice that the Current Deck Display shows the current position of all labware on the deck Figure...

Page 24: ...liquid transfer so click the up arrow next to Tip Handling to collapse the configuration This allows more room for the step configuration Figure 2 8 The text displayed reflects your tip handling confi...

Page 25: ...urns into a hand hold the left mouse button down to move the hand up and down until the depth is as close to 2 00 mm from bottom as you can get Then adjust the height precisely to 2 00 mm using the fo...

Page 26: ...ured in microliters L automatically and indicates the amount of liquid that will be dispensed 100 L into each of the 96 wells so in this case you re dispensing a total of 9600 L into the 96 well micro...

Page 27: ...ith Destination configured Note The method view changes to reflect the amount transferred when the next step is configured The Transfer step configuration is now complete In the next section you will...

Page 28: ...Software User s Manual Chapter 15 4 Move Labware Step To configure the Move Labware step to move your Dest1 labware at position P6 to another deck position 1 Insert a Move Labware step into the Metho...

Page 29: ...onfiguring the Finish Step for information on configuring the Finish step 2 6 1 Handling Configuration Errors If there is a step configuration error in your method when you click on the Finish or any...

Page 30: ...your saved Quick Start method Methods and Project Files Methods are saved as elements of a project file not as separate method files Saving a method only saves the current status of the method It does...

Page 31: ...to watch the method on your screen without actually running the method on an instrument To run your saved method 1 If you do not see the Simulation Display button on the toolbar Figure 2 15 from the...

Page 32: ...ed and the step configuration area is replaced with a three dimensional model of your Biomek 3000 Figure 2 16 The deck confirmation prompt Figure 2 17 appears on top of the main editor Figure 2 16 Bio...

Page 33: ...so be followed in the Method View as steps are highlighted in green as they are executed Note If you are running this method on your instrument instead of the simulator make sure that the physical dec...

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Page 35: ...thod to be customized to your special requirements You can go beyond the predefined project items and instrument configurations manually control the pod restore instrument settings and project files a...

Page 36: ...o the referenced chapter for more information You will work with four of the more commonly used editors and Manual Control in this quick start guide If using Accounts Permissions You must have Develop...

Page 37: ...niques for your specific needs and save them for use in future methods refer to the Biomek Software User s Manual Chapter 9 Understanding and Creating Techniques Liquid Type Editor allows you to creat...

Page 38: ...ation Workstation User s Manual Chapter 14 Manually Controlling the Biomek 3000 in Biomek Software Note Refer to Section 3 8 Manually Controlling the Pod in this quick start guide to learn how to manu...

Page 39: ...anual Chapter 7 Creating and Modifying Tip and Labware Types for further information on using the Labware Type Editor Note Do not run a method outside of the simulator using the labware you create her...

Page 40: ...Biomek Software it is important to copy and then edit the copy of the labware type rather than just editing the predefined labware to meet the new labware specifications 3 Enter NewLabwareQuickStart...

Page 41: ...LabwareQuickStart appears Figure 3 5 Figure 3 5 NewLabwareQuickStart Note Each piece of labware in Labware Types has its own mini editor similar to Figure 3 5 to allow specifications to be revised and...

Page 42: ...terns that may be used or copied to create new patterns The patterns created and stored in Well Patterns are accessible in the Transfer and Combine steps Assume that you want to create a new well patt...

Page 43: ...9 Biomek 3000 Laboratory Automation Workstation Quick Start Guide 2 Choose New New Pattern appears Figure 3 7 Figure 3 7 New Pattern 3 Highlight NewLabwareQuickStart and choose OK Figure 3 8 appears F...

Page 44: ...lter Inc 4 While holding down the Shift key select the wells for the new pattern so it looks like Figure 3 9 Figure 3 9 Wells chosen for the new pattern 5 Choose Rename to rename the new pattern Renam...

Page 45: ...ick Start Guide 6 Enter LabwareQuickStart and choose OK Figure 3 11 appears Figure 3 11 New pattern renamed 7 Choose Save to save LabwareQuickStart in Well Patterns Note Refer to the Biomek Software U...

Page 46: ...cking in methods Enter a reason and your password to check in the method and project refer to the Biomek Software User s Manual Chapter 12 10 Checking In a Method Note If using Accounts Permissions me...

Page 47: ...configure Hardware Setup if you have a new tool that you want to use with your Biomek 3000 instrument You will add a P200L Single Tip Pipette Tool to the list of tools available Adding a tool in Hard...

Page 48: ...he serial number printed on the tool label in Tool Serial Number Note The other fields may be used to modify the performance of the tool However Tool Conversion Slope and Capacity should only be chang...

Page 49: ...trument Deck Editor Deck Editor appears Figure 3 15 Figure 3 15 Deck Editor 2 Select New Deck Choose a name for this deck appears Figure 3 16 Figure 3 16 Choose a name for this deck 3 Enter TwoToolRac...

Page 50: ...eck This deck is now the default deck and is available in the Instrument Setup step Figure 3 17 TwoToolRacks deck with second tool rack added 6 Select Open Deck Select a Deck appears Figure 3 18 Figur...

Page 51: ...kstation User s Manual Chapter 14 Manually Controlling the Biomek 3000 in Biomek Software for additional information Using the following instructions you will use Manual Control to move the pod to a d...

Page 52: ...configured height 4 Select Close Advanced Manual Control Pod1 closes 5 From Manual Control click on P5 The head assembly moves to position P5 on the deck 6 You can click on additional positions to mov...

Page 53: ...this quick start guide You ve completed a small method steps using the editors steps creating some new project items steps to manually control the Biomek 3000 If you ve used the links to other manual...

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