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Beckman Coulter Allegra 25R Instruction Manual Download Page 26

2-2

Preinstallation Requirements

Figure 2-1. Centrifuge Dimensions

ELECTRICAL REQUIREMENTS

208-V, 60-Hz centrifuge  . . . . . . . . . . . . . 187–229 VAC, 16 A, 60 Hz
200-V, 50/60-Hz centrifuge . . . . . . . . .190–210 VAC, 16 A, 50/60 Hz
230-V, 50-Hz centrifuge  . . . . . . . . . . . . . 207–253 VAC, 13 A, 50 Hz

To reduce the risk of electrical shock, this centrifuge uses a 1.8-m 
(6-ft) three-wire electrical cord (attached to the power connector at 
the rear of the centrifuge) and plug to connect the centrifuge to earth-
ground. The plug for use in North America is the NEMA 6-20P; a 
NEMA 6-20R socket (see Figure 2-2) should be available. (Contact 
Beckman Coulter field service for specific information regarding 
local requirements.) To preserve this safety feature:

IMBALANCE 

ENTER 

PULSE 

ACCEL 

DECEL 

TEMP 

RPM 

RCF 

ROTOR 

TIME 

STOP 

START 

FAST 

STOP 

OPEN 

DOOR 

SPEED 

TIME 

TEMP °C

ACC/DEC 

7.6 

3.0 

cm 

in. 

40.5 

16.0 

65.0 

25.6 

7.6 

3.0 

68.5 

27.0 

109.0 

43.0 

Allegra 25R Centrifuge 

Summary of Contents for Allegra 25R

Page 1: ...BA IM 5 Allegra 25R Centrifuge Instruction Manual IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C ACC DEC Allegra 25R Centrifuge ...

Page 2: ...lter les documents joints Atención consulte los documentos adjuntos Attenzione consultare le informazioni allegate On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione Off power Aus Netzverbindung Arrêt mise hors tension Apagado Spento fuori tensione Protective earth ground Schutzleiteranschluß Liaison à la terre Puesta a tierra de protección Collegamento di protezi...

Page 3: ...volve the risk of electric shock or personal injury Make sure that the power switch is off and the centrifuge is disconnected from the main power source and refer such servicing to qualified personnel Do not replace any centrifuge comonents with parts not specified for use on this instrument Electrical Safety To reduce the risk of electrical shock this equipment uses a three wire electrical cord a...

Page 4: ...fuge however unless all necessary safety precautions are taken Observe all cautionary information printed on the original solution containers prior to their use Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain ...

Page 5: ...il 2009 2009 Beckman Coulter Inc Allegra 25R Centrifuge Instruction Manual IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C ACC DEC Allegra 25R Centrifuge ...

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Page 7: ... Centrifuge Function and Safety Features 1 1 Centrifuge Function 1 1 Safety Features 1 2 Centrifuge Chassis 1 3 Housing 1 3 Door 1 3 Rotor Chamber 1 3 Temperature Sensing and Control 1 3 Drive 1 4 Controls and Indicators 1 4 Power Switch 1 4 Control Panel 1 4 Name Rating Plate 1 7 Specifications 1 8 Available Rotors 1 9 SECTION 2 PREINSTALLATION REQUIREMENTS Space and Location Requirements 2 1 Ele...

Page 8: ...on and Deceleration Rates 3 6 Starting a Run 3 7 Changing Parameters During a Run 3 8 Stopping a Run 3 9 SECTION 4 TROUBLESHOOTING User Messages 4 1 Other Possible Problems 4 3 Retrieving Your Sample in Case of Power Failure 4 4 SECTION 5 CARE AND MAINTENANCE General Maintenance 5 1 Cleaning 5 2 Decontamination 5 2 Sterilization and Disinfection 5 3 Storage and Transport 5 3 Supply List 5 4 Replac...

Page 9: ... 1 Centrifuge Dimensions 2 2 Figure 2 2 North American Electrical Socket 2 3 Figure 4 1 Door Latch Override 4 5 Illustrations Page Table 3 1 Acceleration and Deceleration Rates in Minutes Seconds to and from Maximum Speed 3 7 Table 4 1 Error Messages 4 2 Table 4 2 Troubleshooting Chart 4 3 Tables ...

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Page 11: ...ations operation and routine operator care and maintenance We recommend that you read this entire manual especially the SAFETY NOTICE and all safety related information before operating the centrifuge or performing instrument maintenance Section 1 contains system specifications and a brief physical and functional description of the centrifuge including the operating controls and indicators Section...

Page 12: ...ser CONVENTIONS Certain symbols are used in this manual to call out safety related and other important information These international symbols may also be displayed on the centrifuge and are reproduced and described below and on the inside of the front cover NOTES CAUTIONS AND WARNINGS NOTE Used to call attention to important information that should be followed during installation use or servicing...

Page 13: ...service personnel TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components such as keys and displays Key names for example START or ROTOR appear in capital letters within brackets Display names for example SPEED or TIME appear in bold type CFC FREE CENTRIFUGATION To ensure minimal environmental impact no CFCs are used...

Page 14: ...eil numérique n émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage radioelectrique édicté par le Ministère des Communications du Canada RECYCLING LABEL This symbol is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of thi...

Page 15: ...umn centrifugations Processing large numbers of small volume samples in multiwell plates for concentrating tissue culture cells cloning and replicate studies in vitro cytotoxicity studies receptor binding and genetic engineering experimentation Rapid sedimentation of protein precipitates large particles and cell debris Binding studies and separation of whole blood Cell isolation The Allegra 25R is...

Page 16: ...s stopped and the LED light emitting diode on the OPEN DOOR key is lit An imbalance detector monitors the rotor during the run causing automatic shutdown if rotor loads are severely out of balance At low speeds an incorrectly loaded rotor can cause imbalance Rotor instability can also occur if the centrifuge is moved while running or if it is not resting on a level and secure surface To prevent ov...

Page 17: ...TOR CHAMBER The rotor chamber is stainless steel for durability and corrosion resistance The chamber is sealed by a silicone rubber gasket TEMPERATURE SENSING AND CONTROL With the power on the temperature control system is activated when the door is closed and locked Run temperature can be set between 20 and 40 C If no set temperature is entered the instrument auto matically selects the last enter...

Page 18: ...OLS AND INDICATORS POWER SWITCH The power switch located on the back panel controls electrical power to the centrifuge It is also a circuit breaker that will trip to cut off power in the event of a power overload The power switch must be turned on before the chamber door can be opened CONTROL PANEL The control panel see Figure 1 2 mounted at an angle on the centrifuge front for easy visibility and...

Page 19: ...rt duration runs as long as the key is pressed Deceleration at maximum rate begins when the key is released Parameter Keys These keys are used to set up the run Except for the cursor and ENTER keys parameter keys are located beneath the applicable digital displays which show the parameter values as they are input Each key except the cursor keys has an LED in the upper left corner that lights to in...

Page 20: ...perature and deceleration rate made during a run in progress ACCEL Enables selection of one of 10 preset acceleration rates see Table 3 1 in Section 3 available to protect specific gradient and sample to gradient interfaces If no acceleration rate is selected the centrifuge defaults to maximum acceleration DECEL Enables selection of one of 10 preset deceleration rates see Table 3 1 in Section 3 av...

Page 21: ...ol is displayed and elapsed time since run start is displayed After 9 hours and 59 minutes the timer resets to 0 and continues counting elapsed time TEMP C Indicates temperature inside the rotor chamber in C within 2 degrees of the set temperature after equilibration ACC DEC When the ACCEL key is pressed the display flashes indicating that an acceleration rate can be entered when the DECEL key is ...

Page 22: ...mbient temperature for optimum operation 23 C 2 C Humidity restrictions 80 noncondensing Acceleration 10 acceleration profiles Deceleration 10 deceleration profiles Dimensions Width 65 0 cm 25 6 in Depth 68 5 cm 27 0 in Height 40 5 cm 16 0 in Weight 124 0 kg 273 4 lb Ventilation clearances sides and rear 7 6 cm 3 0 in Finishes Control panel coated polycarbonate Housing surfaces urethane paint Elec...

Page 23: ...in radians per second 2 π RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution Rotor Profile and Description Rotor Selection Code Max RPM Max RCF g at rmax Number of Tubes Nominal Capacity Rotor Manual Number TA 15 1 5 Fixed Angle rmax 100 mm A 15 15 000 25 160 30 1 5 2 0 mL BS TB 002 TA 10 250 Fixed Angle rmax 137 mm A 10 10 000 15 300 6 250 mL BS TB 003 TA 14 50 Fix...

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Page 25: ...rom these materials could enter the centrifuge air system and be ignited by the motor Select a location away from heat producing laboratory equipment with sufficient ventilation to allow for heat dissipation Position the centrifuge on a level surface such as a sturdy table or laboratory bench that can support the weight of the centrifuge 124 0 kg 273 4 lb and resist vibration Relative humidity sho...

Page 26: ...ched to the power connector at the rear of the centrifuge and plug to connect the centrifuge to earth ground The plug for use in North America is the NEMA 6 20P a NEMA 6 20R socket see Figure 2 2 should be available Contact Beckman Coulter field service for specific information regarding local requirements To preserve this safety feature IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME ST...

Page 27: ...ure it under load while the drive is operating To ensure safety the centrifuge should be wired to a remote emer gency switch preferably outside the room where the centrifuge is housed or adjacent to the exit from that room in order to disconnect the centrifuge from the main power source in case of a malfunction TRANSPORTATION SAFETY DEVICE The centrifuge is shipped with a transportation safety dev...

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Page 29: ...i further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge with out taking appropriate safety precautions Biosafe containment ...

Page 30: ... T ENTER Set temperature 7 ACCEL S or T ENTER Select acceleration rate You can skip this step and accept the default acceleration rate which is maximum acceleration 8 DECEL S or T ENTER Select deceleration rate You can skip this step and accept the default deceleration rate which is maximum deceleration 9 START Check that all parameters are correct and that the door is closed then press START CAUT...

Page 31: ...unlock the door The centrifuge will not accept this command unless the rotor is at rest and the OPEN DOOR LED is lit 2 Install the rotor according to the rotor manual 3 Gently close the chamber door the automatic latching mechanism engages when the door is nearly closed SETTING RUN PARAMETERS SELECTING A ROTOR Make sure the rotor is seated on the drive shaft and the tie down screw is tight before ...

Page 32: ... You can set parameters for the run SETTING RUN SPEED You can set centrifuge speed in either rpm or RCF for up to the maximum rated speed of the selected rotor Setting RPM Action Result 1 Press RPM The last digit on the SPEED display flashes 2 Press S or T until the required speed appears then press ENTER During centrifugation the SPEED display shows the actual run speed of the rotor in rpm The co...

Page 33: ...utes Action Result 1 Press TIME The last digit on the TIME display flashes 2 Press S or T until the required time appears then press ENTER Timed run set a time between 1 minute and 9 hours 59 minutes If you enter a number between 59 and 99 minutes the centrifuge automatically recalculates the time in hours and minutes Continuous set a time below 0 or above 9 59 SETTING RUN TEMPERATURE You can set ...

Page 34: ...ace Select rates depending on the type of run you are performing for pelleting runs where sample mixing is not a concern maximum deceleration can be used If running delicate gradients a lower setting may be needed Typical acceleration and deceleration rates are listed in Table 3 1 If no rate is selected the centrifuge automatically accelerates and decel erates at maximum Setting Acceleration Rate ...

Page 35: ...tting Deceleration Rate Action Result 1 Press DECEL The ACC DEC display flashes 2 Press S or T until the required deceleration rate appears then press ENTER Entered number appears on the ACC DEC display STARTING A RUN You can start the run with parameters in memory from the previous run or using new or changed parameters that you enter using the procedure described above Accel Decel Rate Accel Dec...

Page 36: ...he run start for a continuous run When the elapsed time reaches 9 hours 59 minutes during a continuous run it resets to 0 and continues counting until STOP or FAST STOP is pressed The ACC DEC display shows the deceleration rate selected for the run WARNING Do not attempt to override the door interlock system while the rotor is spinning CHANGING PARAMETERS DURING A RUN While a run is in progress pa...

Page 37: ...TER The current run speed will be displayed changing to the new value as the rotor accelerates or decelerates to the new speed selected The centrifuge will automatically calculate the corresponding RCF STOPPING A RUN A timed run ends automatically when the TIME display counts down to zero To end a timed or continuous run in progress for any reason Action Result 1 Press STOP Normal deceleration beg...

Page 38: ...ss OPEN DOOR The door can be opened 3 Remove the rotor according to the rotor manual CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories ...

Page 39: ...occurs during operation the rotor will decelerate to a stop and an error code will appear on the SPEED display Messages may result from incorrect input such as exceeding allowable speed for a rotor or from an equipment malfunction Refer to Table 4 1 to determine the nature of the problem If you are unable to correct the problem call Beckman Coulter Field Service To help diagnose and correct the pr...

Page 40: ... YOUR SAMPLE below 84 Thermal cutoff switch activated Deceleration to full stop 1 Turn the power off 2 Check that ambient temperature is within the specified limits 10 to 35 C 3 Check for air inlet and exhaust vent obstruction Clean air inlets and exhaust vents 4 After the motor has cooled down approximately 15 minutes with the door open turn the power on to reset the thermal protector circuit 85 ...

Page 41: ...omplete stop open and close the centrifuge door then restart Drive error mechanical damage Call Beckman Coulter Field Service Rotor cannot achieve set speed Line voltage below rating Have a qualified service person measure line voltage while the instrument is operating Electrical failure Make sure the power cord is securely connected call Beckman Coulter Field Service Motor failure Call Beckman Co...

Page 42: ...the power cord from the main power source No displays or LEDs are lit 2 Make sure that the rotor is not spinning No sound or vibration comes from the centrifuge WARNING Never attempt to override the door interlock system while the rotor is spinning 3 Use a small flat screwdriver to remove the cover from the override key opening on the front panel see Figure 4 1 4 Insert the supplied override key i...

Page 43: ...tch Override A 10 mm square head socket wrench can be used if the provided override key is unavailable IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C ACC DEC Allegra 25R Centrifuge ...

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Page 45: ...eckman Coulter Field Service GENERAL MAINTENANCE Perform the following procedures regularly to ensure satisfactory performance and long service life of the centrifuge At least once a week depending on usage inspect the rotor chamber for accumulations of sample dust and glass particles from broken sample tubes Clean as required see CLEANING below as these accumulations can result in rotor vibration...

Page 46: ...aper towel To prevent accumulations of sample dust and or glass particles from broken sample tubes from damaging rotor pins causing rotor vibrations remove the rotor and clean the chamber often Spray the centrifuge chamber with an antistatic solution and wipe it clean Or use an antistatic wipe Clean the centrifuge case and door by wiping with a damp cloth or washing with Solution 555 Do not use ac...

Page 47: ... disinfection is a concern consult your laboratory safety officer regarding proper methods to use STORAGE AND TRANSPORT To ensure that the centrifuge does not get damaged contact Beckman Coulter Field Service for specific instructions and or assistance in preparing the equipment for transport or long term storage Temperature and humidity requirements for storage should meet the environmental requi...

Page 48: ...er of this manual for information about ordering parts supplies and publica tions A partial list is given below for your convenience REPLACEMENT PARTS Door latch override key 368247 Rotor tie down screw 368245 T handle wrench rotor fastening 368246 SUPPLIES Spinkote lubricant 2 oz 306812 Silicone vacuum grease 1 oz 335148 Beckman Solution 555 1 qt 339555 ...

Page 49: ... at its election replace such component or accessory What consti tutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the trans portation charges collect unless the ...

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Page 55: ...Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com 2009 Beckman Coulter Inc All rights reserved ...

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