36
BD CARV II Installation and User’s Guide
Main CARV II Screen
When the CARV II is powered on, the first screen displayed is the main screen. If,
however, the touch pad is being pressed, the calibration screen is displayed. In
this case, after the instructions on the screen have been followed, the main screen
is displayed. An example of the main screen is shown in Figure 3-1 on page 36
(of course the version number may differ).
Figure 3-1
Main CARV II Screen
The layout of the main screen represents the light path through the main unit.
The light source is the light bulb outside of the box. The illumination light passes
through the shutter and excitation wheel (the eight-position circle below the
shutter graphic). The light path is then reflected to the right by the five-position
epidichroic wheel (which will be referred to as the dichroic wheel). In its
initialized state, the CARV II now presents the FRAP iris to the light path; the iris
is in its full open setting so there is no loss of intensity. The light path now leaves
the CARV II and enters the microscope where it is reflected off of the sample and
returns through the same opening. Since the reflected light is typically of a
different wavelength, it passes through the dichroic wheel and then the emission
wheel on its way to the camera.
