WXG-4
I
2
Measurement
1. Open the light shield lid and place the test tube into the sample
chamber. Note, the bubble trap is upward.
2. Close the lid, clockwise or anticlockwise rotate vernier knob until
the visual field appears the uniform brightness. Record the
measured value.
3. Repeat the above measurements twice and calculate the average
of measured values.
Calculation
If the measured values are positive angle (dextrorotatory substance),
minus the value of the reference point shall be the actual measured
value of the sample.
If the measured values are negative angle (levorotatory substance),
minus the 180° shall be the actual value of the sample.
Determination of the Concentration
If you need to determine the purity, concentration or proportion of the
sample, please using the formula below.
α
= [
α
] LC
Where:
α
= Optical rotation
[
α
] = Specific rotation
L
= Tube length (dm)
C = Concentration (g/L)
Replacing the Sodium Lamp
Before replacing the sodium lamp, be sure to disconnect the power
plug from the wall outlet.
1. Wait for the sodium lamp to cool down, remove the lamp cover.
2. Pull the sodium lamp from the socket and insert a new one.
3. Replace the lamp cover again in such a way that the window
point in the direction of the polarizer.
Appendix
Optional Accessories
Order Code
Description
LAMP 589
Sodium lamp, 4-pin, wavelength 589 nm
POLTUBE 100
Test tube, 100 (L) × 27 (Dia.) mm
POLTUBE 200
Test tube, 200 (L) × 27 (Dia.) mm
Specifications
Range
± 180°
Scale Value
1°
Vernier
0.05°
Magnifier
3X
Light Source
Sodium lamp
Wavelength
589 nm
Tube Length
50, 100 or 200 mm
Operating Temperature 0 to 40° C (32 to 104° F)
Storage Temperature
0 to 60° C (32 to 140° F)
Relative Humidity
< 80% (non-condensing)
Power
AC 220V/50Hz
Dimensions
500 (L) × 135 (W) × 330 (H) mm
(19.6 × 5.3 × 13 in.)
Weight
5 kg (11 lb)
