Instruction for Use HISTO TYPE Rainbow QS6
Version: 02/2021
Page 5 of 16
6.3
Entering sample information into PlexTyper
®
Software
It is mandatory to use PlexTyper
®
Software to analyse HISTO TYPE Rainbow data. It is advisable
to enter the sample information into PlexTyper
®
before setting up the PCR amplification to obtain
the unique RUN ID. All import files must be prefixed with a PT number.
When saving a test in PlexTyper
®
, the software assigns a
Run ID
to the test which is displayed in
red in the summary window shown below. This is a consecutive number prefixed with PT (PT1, PT2,
PT3….) which is linked to the sample identity and is used as a prefix on the PCR machine export file
to correctly link the PCR export to the sample identity.
This Run ID should be used when setting up the file identity in the PCR machine. If the PCR excel
export file is not pre-labelled with the RUN ID the file can be manually prefixed with the correct PT
Run ID before import into PlexTyper
®
.
6.4
Amplification
A pre-mix consisting of Plex Mix, water and DNA is made for the amplification that is subsequently
dispensed in the wells 1-95. In well 96 there is the negative control (no template control = NTC)
which should only contain water and Plex Mix. For other DNA concentrations the pre-mix has to be
modified accordingly (see below).
•
The reaction volume for each PCR preparation is 10 µl
•
For a single well the following reagents must be pipetted into a reaction tube:
2 µl
Plex Mix
1 µl
DNA specimen
7 µl
Molecular grade water
A
negative control (NTC)
should be performed. Therefore prepare a PCR reaction with molecular
grade water instead of DNA.
2 µl
Plex Mix
8 µl
Molecular grade water
