Applied Biosystems GeneAmp PCR System 9700, User Manual

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4-8 Running PCR Samples
Viewing Method
Information
You can view the Method Information screen during a run by pressing Info. Press
Return to return to the Run Time screen.
Pausing a Run
You can manually pause a run for a ten minute period of time during a run by pressing
Pause (Figure 4-1). If you want to specify a different period of time for a pause, see
“Setting the Pause Time Out” on page 3-10.
Press Resume to resume running a method before a pause expires.
Note You can pause a run in order to add a reagent. Do not to touch the sample block or the
bottom of the heated cover during a pause.
Figure 4-1 Pause screen
Your samples remain at the temperature of the instrument when you pause the run.
The time remaining in a pause appears at the bottom of the screen in
minutes:seconds format. It decrements to zero, and the paused run resumes at the
point where you paused it.
02:32 PM Information 55.2°C
User: lisa
Run started at 01:32:30 PM, 01/01/00.
Run will end at 06:35:30 PM, 01/01/00.
Reaction vol: 50 µL Ramp Speed: 9600
Method: XL PCR
F1 F3 F4 F5 F2
Return
Sample 56.7 Cycle 1 of 25 HOT
55.0
94.0
2:00
10:00
55.0
0:30
72.0
0:30
72.0
5:00
4.0
94.0
0:30
Paused. Will resume in mm:ss
F1 F3 F4 F5 F2
Resume