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Appendix E

Documentation and support

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Automated Thermal Cycler (ATC) System User Guide

Summary of Contents for A30207

Page 1: ...edures Automated Thermal Cycler ATC System USER GUIDE Installation use and maintenance Catalog Numbers A30207 A30208 A31486 A31487 A31488 A31489 A31490 A31491 A33976 A33977 A33978 A33979 A33980 A33981 and A33982 Publication Number MAN0014558 Revision B 0 ...

Page 2: ...NCLUDING YOUR USE OF IT Revision history Pub No MAN0014558 Revision Date Description B 0 17 June 2021 Addition of the Fleet software appendix and a sentence in the product description regarding the Fleet software A 0 11 April 2017 New document Describes installation operation and maintenance of the Automated Thermal Cycler ATC System Important Licensing Information This product may be covered by o...

Page 3: ...stand alone using computer 15 Program and execute a run on the ATC 18 Connect to multiple instruments using the desktop software 22 Firmware upgrades 23 CHAPTER 3 Operate the Automated Thermal Cycler ATC System as part of a robotic system 26 Set up the Automated Thermal Cycler ATC System on a robotic system 26 Guidelines for robotic configurations 28 Connect Automated Thermal Cycler to a robotic s...

Page 4: ...overview 41 Mandatory commands 42 Abort 42 DoContinue 42 GetDeviceIdentification 43 GetStatus 44 Initialize 44 LockDevice 44 Pause 45 Reset 45 UnlockDevice 46 Required Commands for PCR Cyclers 46 Delay 46 GetParameters 47 PrepareForInput 47 PrepareForOutput 48 SetParameters 48 Optional commands for PCR Cyclers 49 GetConfiguration 49 SetConfiguration 50 Optional commands for other device classes 51...

Page 5: ...r instruments not manufactured by Thermo Fisher Scientific 62 Instrument safety 62 General 62 Physical injury 62 Electrical safety 63 Cleaning and decontamination 63 Instrument component and accessory disposal 63 Safety and electromagnetic compatibility EMC standards 63 Safety 64 EMC 64 Environmental design 65 RoHS 65 Chemical safety 66 Biological hazard safety 67 APPENDIX E Documentation and supp...

Page 6: ...tible with the Applied Biosystems Thermal Cycler Fleet Control Software The Automated Thermal Cycler ATC System supports 96 Well 384 Well and 0 2 mL sample block configuration and includes three components A thermal block and heated cover that constitute the thermal cycler unit A power box A power cable is used for connecting the thermal cycler to the power box For details on Automated Thermal Cyc...

Page 7: ...Thermal Cycler Site requirements The Automated Thermal Cycler ATC System is for indoor use Ensure that the installation site Meets the spatial and weight requirements see System specifications on page 9 Meets environmental requirements see Environmental requirements on page 8 Is within 1 m 3 ft of an AC power source receptacle Is away from water Chapter 1 About the Automated Thermal Cycler ATC Sys...

Page 8: ... that has non conductive pollutants only such as dust particles or wood chips Typical environments with a Pollution Degree II rating are laboratories and sales and commercial areas Technical specifications Table 4 Automated Thermal Cycler ATC System technical specifications Feature Specification Block format 96 well 0 2 mL 384 well 0 02 mL Maximum block ramp rate 1 3 5 C sec 96 well 2 8 C sec 384 ...

Page 9: ...10 100 µL 96 well 5 10 µL 384 well Instrument memory USB Power 100 240 V 50 60 Hz Max 600 W 1 Reaction volume 1 µL System specifications Table 5 Physical dimensions and weight and power consumption Dimension Instrument footprint Recommended clearance Thermal module Control box Thermal module Control box Height 133 6 mm 5 3 in 76 4 mm 3 0 in N A N A Width 179 6 mm 7 1 in 257 0 mm 10 1 in N A N A De...

Page 10: ...er button 3 Ethernet port 4 Automated Thermal Cycler ATC System DSUB 5 USB port 1 Figure 2 Back panel of the ATC block module 1 Automated Thermal Cycler ATC System DSUB Chapter 1 About the Automated Thermal Cycler ATC System Location of power points and ports 1 10 Automated Thermal Cycler ATC System User Guide ...

Page 11: ...d adaptor amber color already installed Note ATC plate adaptors are only installed required on the 96 well block Adaptors are not required for the 384 well block Figure 3 Automated Thermal Cycler 96 well block with full skirted plate adaptor installed To uninstall the full skirted adaptor follow the instructions in Figure 4 Chapter 1 About the Automated Thermal Cycler ATC System Use ATC plate adap...

Page 12: ...hlighted in red 2 Grab the bottom of the adaptor and tilt up 3 Remove the adaptor To install full skirted plate adaptor follow the instructions in Figure 5 Chapter 1 About the Automated Thermal Cycler ATC System Use ATC plate adaptors 1 12 Automated Thermal Cycler ATC System User Guide ...

Page 13: ... 2 Push the two tabs allowing it to be inserted into the frame 3 Ensure the full skirted plate adaptor sits freely under the frame 4 Full skirted plate adaptor is installed To install the semi skirted plate adaptor follow the instructions in Figure 6 Chapter 1 About the Automated Thermal Cycler ATC System Use ATC plate adaptors 1 Automated Thermal Cycler ATC System User Guide 13 ...

Page 14: ...irted plate adaptor to the gap between the Alu top plate and drip pan 2 Slot in the adaptor and center it 3 Snap fit the ejector buttons 4 There are four ejector buttons Chapter 1 About the Automated Thermal Cycler ATC System Use ATC plate adaptors 1 14 Automated Thermal Cycler ATC System User Guide ...

Page 15: ...ctor is securely fastened 2 Connect the power cable into the back of the ATC control module Figure 1 item 1 3 Connect the ethernet cable into the control module Figure 1 item 3 and into the computer network that you will be using to control the ATC 4 Power on the ATC Operate ATC as stand alone using computer Make sure all hardware connections are completed 1 Download the ATC desktop software at th...

Page 16: ...et cable to your computer Note It will take approximately 1 5 minutes for the ATC to initialize before it can connect to the software 4 Once the ATC instrument has initialized click Discover to identify all devices connected to the same local network A pop up window will appear 5 Double click on the identified instrument name Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alo...

Page 17: ...ime monitoring temperature curves to see black window legend with graph related information 8 Click Start to open Protocol Editor Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alone system Operate ATC as stand alone using computer 2 Automated Thermal Cycler ATC System User Guide 17 ...

Page 18: ...uence of stages Specify the number of cycles required for the particular stage 3 In the Steps field specify the temperature set points ramp rate and duration The ramp rate is indicated as a where 100 corresponds to a peak block ramp rate of 3 5C s Type in 1 in the Hold Time field to achieve an infinity hold time 4 Enter cover temperature and sample volume 5 Click Start Run to start immediately or ...

Page 19: ...ned using a text editor in order to see the content otherwise they might appear empty if opened with a default browser application When exporting to SiLA format no white space is allowed in stage label and protocol file name Only alpha numeric characters and _ are accepted Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alone system Program and execute a run on the ATC 2 Autom...

Page 20: ...ocol while the software control and monitor the instrument running without interfering with the current run Figure 11 Instrument not ready for run warning Figure 12 Run Protocol Edit Only mode Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alone system Program and execute a run on the ATC 2 20 Automated Thermal Cycler ATC System User Guide ...

Page 21: ...s monitoring If the lid is opened while a run is in progress a warning message will open Figure 14 Alert of run in progress Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alone system Program and execute a run on the ATC 2 Automated Thermal Cycler ATC System User Guide 21 ...

Page 22: ...ftware by repeatedly double clicking on the software shortcut In each software instance discover and connect to a different ATC 3 To launch a number of instances of the ATC Demo software at the same time double click the ATC Demo desktop icon FigureX repeatedly to open multiple windows of the application and then select from each specific application window using Discovery button the instrument yo...

Page 23: ...ts and we do not recommend that a single ATC is connected to multiple computers Firmware upgrades 1 Copy the atc X X X update firmware file into the USB thumb drive and plug this into the ATC control box USB port 2 Connect the ATC to the ATC demo software as described in Operate ATC as stand alone using computer on page 15 3 Click Upgrade Chapter 2 Operate the Automated Thermal Cycler ATC System a...

Page 24: ...e click the version you want to initiate the upgrade 5 Firmware upgrade will initialize and should take 2 3 minutes to complete Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alone system Firmware upgrades 2 24 Automated Thermal Cycler ATC System User Guide ...

Page 25: ...nnected from the instrument Wait 2 3 minutes for instrument to re initialize and then you can connect to the instrument again Chapter 2 Operate the Automated Thermal Cycler ATC System as a stand alone system Firmware upgrades 2 Automated Thermal Cycler ATC System User Guide 25 ...

Page 26: ...ng a protocol Closing the heated cover is optional when the Automated Thermal Cycler ATC System is used as an incubator Set up the Automated Thermal Cycler ATC System on a robotic system 1 Unbox the Automated Thermal Cycler ATC System 2 Place the thermal cycler and the power control box at the intended location within the robotic environment as per the recommended guidelines See Guidelines for rob...

Page 27: ...s for the list of operating commands to be used by the host software Note Non developers who want to integrate the Automated Thermal Cycler ATC System into their robotic platform but do not have the ability to develop the software contact your robotic s manufacturer for installation instructions Chapter 3 Operate the Automated Thermal Cycler ATC System as part of a robotic system Set up the Automa...

Page 28: ...sing information For the most recent information regarding robot compatibility driver information and the full instrument user guide please visit thermofisher com atc or thermofisher com Perform a thermal protocol run See Appendix B Command dictionaries for details on defining the various cycles steps and stages of a protocol run Note Ensure that the consumable is properly sealed with a compatible...

Page 29: ...CR Index 2 Repeat 10 Step Identifier 1 Ramp Rate 100 Temperature 95 0 Temperature Temperature 95 0 Temperature Temperature 95 0 Temperature Ramp Hold HoldTime 30 0 Step Step Identifier 2 Ramp Rate 100 Temperature 60 0 Temperature Temperature 60 0 Temperature Temperature 60 0 Temperature Ramp Hold HoldTime 30 0 Step Stage Protocol IMPORTANT Do not copy paste the example protocol as the command synt...

Page 30: ...ocol run ExecuteProtocol requestId RequestID lockId LockID SampleVolume 0 RunMode CoverTemperature 0 Delay 0 Protocol MyRunProtocol RunTitle MyRun Optional View the progress of the thermal protocol run View the progress of the thermal protocol by subscribing to two main topics SubScribe requestId RequestID lockId LockID Event Run SubScribe requestId RequestID lockId LockID Event Temperature Chapte...

Page 31: ...n with the Run ID of the protocol being run AbortRun requestId RequestID lockId LockID RunTitle MyRun Optional Control the automated heated cover lid Control the heated cover lid with the following SiLA commands To open the automated lid OpenDoor requestId RequestID lockId LockID To close the automated lid CloseDoor requestId RequestID lockId LockID Chapter 3 Operate the Automated Thermal Cycler A...

Page 32: ... Clean the sample wells once a month or as needed WARNING Always wear protective glasses and gloves when servicing the instrument Also make sure you disconnect the instrument from AC line power before you begin any service procedure Always wear protective glasses and gloves when servicing the instrument Also make sure you disconnect the instrument from AC line power before you begin any service pr...

Page 33: ...xcessive use of the solution however can corrode the sample block material To prevent damage to the sample block Avoid applying excessive amounts of bleach solution If possible instead of using a squeeze bottle or soaked cloth use an atomizer to deliver the solution to the wells of the block s After treating with bleach solution rinse the sample block s thoroughly using deionized water Note Removi...

Page 34: ...ry skin and cause irritation Exposure may cause central nervous system effects such as drowsiness dizziness and headache Read the MSDS and follow the handling instructions 3 Remove any remaining isopropanol from the cover Note If the platen becomes contaminated with amplified DNA then raise the heated cover to the cleaning position wipe the platen with a cloth or cotton swab soaked in bleach then ...

Page 35: ... the instrument powers on Note Fuse failure can result from fluctuations in the supplied power to the system To prevent further failures consider installing an electrical protective device such as a UPS or a surge protector If issues with the fuse persist contact Support Separate the heated cover from the block 1 Power off the control box and disconnect the cable between the thermal block and cont...

Page 36: ...h a Hex Allen key Note The tightening torque is 8 00 kgfcm 10 4 Remove the six M3 x 6 countersunk screws located at the rear of the block with a Hex Allen key Note The tightening torque is 4 00 kgfcm 10 Chapter 4 Maintain the Automated Thermal Cycler ATC System Separate the heated cover from the block 4 36 Automated Thermal Cycler ATC System User Guide ...

Page 37: ...al Cycler ATC System 7 Move the heated cover to the close position using a slotted screwdriver in the anti clockwise direction 8 Remove the two M3 x 6 button screws located and the transparent plastic cover with a Hex Allen key then carefully unplug the heated cover connector and secure the flex cable by scotch tape or its equivalent Note The tightening torque is 8 00 kgfcm 10 Chapter 4 Maintain t...

Page 38: ...orque is 8 00 kgfcm 10 10 Separate the auto module from the base module as shown in the image to reveal the wire connection and unplug the front closing sensor connector back opening sensor connector and the motor connector Chapter 4 Maintain the Automated Thermal Cycler ATC System Separate the heated cover from the block 4 38 Automated Thermal Cycler ATC System User Guide ...

Page 39: ...tor 2 Back opening sensor connector 3 mMor connector 1 2 1 Auto Module 2 Base Module Chapter 4 Maintain the Automated Thermal Cycler ATC System Separate the heated cover from the block 4 Automated Thermal Cycler ATC System User Guide 39 ...

Page 40: ... thermofisher com Item Cat No 96 Well Temperature Verification Kit 0 2 ML 9 channels 4350703 Automated Thermal Cycler ATC System 1 m cable A31482 Automated Thermal Cycler ATC System 3 m cable A31483 Automated Thermal Cycler ATC System Adaptor connector A31485 A 40 Automated Thermal Cycler ATC System User Guide ...

Page 41: ...asses 51 Specific commands 52 SiLA return codes 56 Command directory overview Delay CloseDoor ExecuteProtocol GetDeviceDetails GetParameters GetProtocolDefinition GetProtocolDetails OpenDoor PrepareForInput PrepareForOutput SetParameters StopProtocol SubScribe UnSubScribe Abort DoContinue GetDeviceIdentification GetStatus Initialize B Automated Thermal Cycler ATC System User Guide 41 ...

Page 42: ...er to the SiLA Device Control Data Interface Specification for further information Note Upon request Thermo Fisher Scientific can provide a copy of a generic command library which is not coded in SILA Abort This commands aborts execution of any command currently being executed including a running protocol and all queued asynchronous commands Device Abort requestId requestId lockId lockId Name Type...

Page 43: ...dentificati on out Example Device GetDeviceIdentification requestId RequestID lockId LockID deviceDescription out Note In the SiLA Test suite out parameters have to be marked by an out behind the parameter name The synchronous command returns an XML string formatted as follows xml version 1 0 DeviceDescription DeviceFirmwareVersion 0 4 6 firmware on infoteam SiLA Library 1 3 0 DeviceManufacturer T...

Page 44: ...false out Example Device GetStatus requestId RequestID deviceIdout stateout subStatesout lockedout PMSIdout currentTimeout Initialize This command is allowed only in the state standby It opens the connection to the Instrument authentication Device Initialize requestId requestId lockId lockId Name Type Nullable In Out requestId Int false in lockId String true in Example Device Initialize requestId ...

Page 45: ...alid only in the state idle It puts the device into a standby mode Device Pause requestId requestId lockId lockId Name Type Nullable In Out requestId Int false in lockId String true in Example Device Pause requestId RequestID lockId LockID Reset This command closes an open connection to the SCPI server and registers the specified event receiverURI Device Reset requestId requestId lockId lockId Nam...

Page 46: ...ovider for the specified lockId Device UnlockDevice requestId requestId lockId lockId Name Type Nullable In Out requestId Int false in lockId String true in Example Device UnlockDevice requestId RequestID lockId LockID Required Commands for PCR Cyclers Delay This command waits for a specified duration and returns Device Delay requestId requestId lockId lockId duration PT5S Name Type Nullable In Ou...

Page 47: ...0 encoding utf 8 ResponseData xmlns xsi http www w3 org 2001 XMLSchema instance xsi noNamespaceSchemaLocation http sila coop schemata ResponseType_1 2 xsd ParameterSet Parameter name ProtocolName ParameterType String String MyRunProtocol String Parameter Parameter name ProtocolDefinition parameterType String String Protocol ProtocolName MyRunProtocol Protocol String Parameter ParameterSet Response...

Page 48: ...on by the ExecuteProtocol command Device SetParameters requestId requestId lockId lockId paramsXML Name Type Nullable In Out requestId Int false in lockId String true in Example Device SetParameters requestId RequestID lockId LockID paramsXML xml version 1 0 encoding utf 8 Protocol ProtocolName MyRunProtocol RunMode Fast Volume 10 Stage Label PreCycle Index 1 Step Identifier 1 Ramp Rate 100 0 Temp...

Page 49: ...kId lockId configLevel1 password Name Type Nullable In Out requestId Int false in lockId String true in configLevel Int false in password String True in Example Device GetConfiguration requestId RequestID lockId LockID configLevel1 password Response Data xml version 1 0 encoding utf 8 ResponseData xmlns xsi http www w3 org 2001 XMLSchema instance xsi noNamespaceSchemaLocation http sila coop schema...

Page 50: ...arameter Parameter name NickName parameterType String String Nick String Parameter Parameter name Time parameterType Int32 Int32 1446484761 Int32 Parameter ParameterSet ResponseData SetConfiguration This command can be used to change some of the data shown in GetConfiguration the nickname and the time in seconds since epoch Jan 1 1970 Device SetConfiguration requestId requestId lockId lockId confi...

Page 51: ...d lockId Name Type Nullable In Out requestId Int false in lockId String true in Example Device OpenDoor requestId RequestID lockId LockID CloseDoor This command closes the lid Device CloseDoor requestId requestId lockId lockId Name Type Nullable In Out requestId Int false in lockId String true in Example Device CloseDoor requestId RequestID lockId LockID Appendix B Command dictionaries Optional co...

Page 52: ...ring false in RunTitle String False in Example ExecuteProtocol requestId RequestID lockId LockID SampleVolume 10 RunMode CoverTemperature 105 Delay 0 Protocol MyRunProtocol RunTitle MyRun Note Based on the required protocol the parameters SampleVolume RunMode and CoverTemperature can be set by the user Delay is optional GetDeviceDetails This command returns the status information from the instrume...

Page 53: ... device then depending on the parameter type all parameters in the returned XML will be empty or 0 Device GetProtocolDefinition requestId requestId lockId lockId ProtocolName MyRunProtocol Name Type Nullable In Out requestId Int false in lockId String true in ProtocolName String True in Example Device GetProtocolDefinition requestId RequestID lockId LockID ProtocolName MyRunProtocol Response Data ...

Page 54: ...esponse Data xml version 1 0 encoding utf 8 ResponseData xmlns xsi http www w3 org 2001 XMLSchema instance xsi noNamespaceSchemaLocation http sila coop schemata ResponseType_1 2 xsd ParameterSet Parameter name ProtocolTime parameterType Duration Duration PT372S Duration Parameter Parameter name RemainingTime parameterType Duration Duration PT0S Duration Parameter Parameter name RunMode parameterTy...

Page 55: ...be monitored Device SubScribe requestId requestId lockId lockId Name Type Nullable In Out requestId Int false in lockId String true in Event String false in Example Device SubScribe requestId RequestID lockId LockID Event Run Device SubScribe requestId RequestID lockId LockID Event Temperature Device SubScribe requestId RequestID lockId LockID Event Error Device SubScribe requestId RequestID lockI...

Page 56: ...kID Event Error Device UnSubScribe requestId RequestID lockId LockID Event Time SiLA return codes If applicable the standard SiLA Return Codes are used to indicate successful or faulty command execution There are two specific return codes 1001 Error on connection to the SCPI server refer to the error message for detailed information 1002 The underlying SCPI command returned an error refer to the e...

Page 57: ...ed Biosystems Thermal Cyclers The Thermal Cycler Fleet Control Software can be purchased separately and is not included with the Automated Thermal Cycler ATC System For more information visit thermofisher com Connect the thermal cycler to the network 1 Connect the thermal cycler to your network using an Ethernet cable 2 Go to Settings Menu Admin Menu Set IP Address to set up your thermal cycler ne...

Page 58: ...fety Data Sheets SDSs and use appropriate personal protective equipment gloves gowns eye protection and so on To obtain SDSs see the Documentation and Support section in this document Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information In this document the hazard symbol is used along with one of the following us...

Page 59: ...posal provision and contact customer service for information about responsible disposal options Ne pas éliminer ce produit avec les déchets usuels non soumis au tri sélectif MISE EN GARDE Pour minimiser les conséquences négatives sur l environnement à la suite de l élimination de déchets électroniques ne pas éliminer ce déchet électronique avec les déchets usuels non soumis au tri sélectif Se conf...

Page 60: ...hazard See the following table for safety alerts found on the instrument English Français CAUTION Hazardous chemicals Read the Safety Data Sheets SDSs before handling MISE EN GARDE Produits chimiques dangereux Lire les fiches signalétiques FS avant de manipuler les produits CAUTION Hazardous waste Refer to SDS s and local regulations for handling and disposal MISE EN GARDE Déchets dangereux Lire l...

Page 61: ...Appendix D Safety Safety alerts on this instrument D Automated Thermal Cycler ATC System User Guide 61 ...

Page 62: ...ument protective covers If you remove the protective instrument panels or disable interlock devices you may be exposed to serious hazards including but not limited to severe electrical shock laser exposure crushing or chemical exposure Physical injury CAUTION Moving Parts Moving parts can crush pinch and cut Keep hands clear of moving parts while operating the instrument Disconnect power before se...

Page 63: ... of the equipment or with material contained in the equipment The instrument is properly decontaminated a if hazardous material is spilled onto or into the equipment and or b prior to having the instrument serviced at your facility or sending the instrument for repair maintenance trade in disposal or termination of a loan decontamination forms may be requested from customer service Before using an...

Page 64: ...strial Scientific and Medical ISM Radiofrequency Equipment ICES 001 Issue 4 Industrial Scientific and Medical ISM Radio Frequency Generators FCC Part 15 Subpart B 47 CFR U S Standard Radio Frequency Devices This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules and CISPR 11 Class A These limits are designed to provide re...

Page 65: ...n RoHS Directive Restriction of hazardous substances in electrical and electronic equipment Directive 2006 66 EC European Union Battery Directive MII Order 39 PRC Management Methods for Controlling Pollution by Electronic Information Products RoHS Appendix D Safety Safety and electromagnetic compatibility EMC standards D Automated Thermal Cycler ATC System User Guide 65 ...

Page 66: ... for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended in the SDS Handle chemical wastes in a fume hood Ensure use of primary and secondary waste containers A primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste materi...

Page 67: ...equirements before working with potentially biohazardous materials Follow all applicable local state provincial and or national regulations The following references provide general guidelines when handling biological samples in laboratory environment U S Department of Health and Human Services Biosafety in Microbiological and Biomedical Laboratories BMBL 5th Edition HHS Publication No CDC 21 1112 ...

Page 68: ...Ms and end users Note For additional documentation see Customer and technical support on page 68 Customer and technical support Visit thermofisher com support for the latest service and support information Worldwide contact telephone numbers Product support information Product FAQs Software patches and updates Training for many applications and instruments Order and web support Product documentati...

Page 69: ...fe Technologies General Terms and Conditions of Sale at www thermofisher com us en home global terms and conditions html If you have any questions please contact Life Technologies at www thermofisher com support Appendix E Documentation and support Limited product warranty E Automated Thermal Cycler ATC System User Guide 69 ...

Page 70: ...Remora_ATC_UG_MAN0014558 v5 GUID 5867C4D2 1F0A 4BD5 8C4A 27A7DCBD0FA6 2019 08 27 20 27 44 en 02 35 08 554 01 00 thermofisher com support thermofisher com askaquestion thermofisher com 17 June 2021 ...

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