Applied Biosystems 2720, User Manual

Page: 38 / 151

30 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 3 Temperature non-uniformity test
7. Slide the heated cover back. Repeat steps 4 through 7 of the “Setting up the 0.2-
mL probe assembly” on page 27 and steps 1 through 6 of this procedure.
Complete these steps for all 8 wells to be tested: C4, C9, F4, F9, A1, A12, H1,
H12.
8. The GeneAmp
®
9700 thermal cycler evaluates the uniformity of the sample
block temperature for the setpoint values that you entered and displays the
results. A summary screen opens at the end of the test.
If you entered values on the Temperature Non-Uniformity Test Data Sheet
(
page 33 ), compare those values with the actual test results.
9. Press
F1
(Accept).
Evaluating the
results
When the thermal cycler completes the temperature non-uniformity test, the TNU
Performance screen opens. See the table below to evaluate the results.
Well
F1 F3 F4 F5 F2
Accept Cancel
xx.x C4 xx.x
xx.x C9 xx.x
xx.x F4 xx.x
xx.x F9 xx.x
Well
xx.x A1 xx.x
xx.x A12 xx.x
xx.x H1 xx.x
xx.x H12 xx.x
37°C
94°C
37°C
94°C
If the... Then...
Temperature of the
sample block wells is
uniform “Pass” appears after each setpoint temperature.
Temperature variation of
the sample block wells
exceeds performance
specifications “Fail” appears after the setpoint temperature(s) for which the
test failed.
• If the test fails, repeat the procedure to make sure that the
thermometer was not misread or that data-entry errors
were not made.
• If the test fails again, contact Applied Biosystems Technical
Support. See “How to obtain support” on page viii .
TNU Performance
TNU at 94°C is xx.xx - Pass
TNU at 37°C is xx.xx - Pass
F1 F3 F4 F5 F2
Cancel
TNU Performance
TNU at 94°C is xx.xx - Fail
TNU at 37°C is xx.xx - Fail
F1 F3 F4 F5 F2
Cancel