Applied Biosystems 2720, User Manual

Page: 37 / 151

29 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 3 Temperature non-uniformity test
2. When the sample block reaches 94 °C, the TNU Performance screen counts
down the time until the setpoint is stable.
3. When the “Stabilizing at setpoint” value decreases to 0, read the digital
thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the Model 4500 digital thermometer.
4. Using the numeric keys, enter the value that is displayed on the digital
thermometer in the “Enter actual block temperature” field.
The digital thermometer displays a four-digit value. Round off the value to three
digits before you enter it in the TNU Performance screen.
Record the value on the Temperature Non-Uniformity Test Data Sheet ( page 33 )
to keep a permanent record of the test.
5. Press
Enter
. The following TNU Performance screen opens and the system
automatically begins decreasing its setpoint temperature to 37 °C.
6. When the setpoint temperature reaches 37 °C, the TNU Performance screen
opens with the following prompt:
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Stabilizing at setpoint x:xx
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
F1 F3 F4 F5 F2
Cancel
Enter actual block temperature 00.0
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
Setpoint is 37°C
Sample must be within 1.0°C of setpoint
F1 F3 F4 F5 F2
Cancel
TNU Performance
Sample temp = xx.x°C Cover temp = xxx°C
F1 F3 F4 F5 F2
Cancel
Place probe in well xx
Press Run
Run