Applied Biosystems 2720, User Manual

Page: 35 / 151

27 Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 3 Temperature non-uniformity test
Temperature non-uniformity test
Use this test to verify the temperature uniformity of the GeneAmp 9700 thermal
cycler with a 0.2-mL sample block module. The test consists of the following
procedures, which you must perform in sequence:
Setting up the 0.2-mL probe assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Configuring the GeneAmp
®
9700 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Running the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Ending the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
PHYSICAL INJURY HAZARD. Hot Surface. Use care when
working around the heated cover and sample block to avoid being burned by hot
components.
Required
equipment
This test requires the 0.2-mL Sample Block Module Temperature Verification Kit
(PN 4317939), which includes:
• Cotton swabs
• Light mineral oil
• 9700 temperature verification frame
• 0.2-mL probe assembly
• Model 4500 digital thermometer with a 9V battery installed
Setting up the
0.2-mL probe
assembly
1. If the heated cover is in the forward position, lift the lever, then slide the heated
cover back.
2. Place the 9700 temperature verification frame on the sample block.
3. Use a cotton swab to coat the following wells with mineral oil:
A1, A12, C4, C9, F4, F9, H1, H12
4. Place the 0.2-mL probe assembly into well C4, then as the test progresses, move
the 0.2-mL probe assembly to each of the test wells.
5. Thread the probe wire through the channel in the 9700 temperature verification
frame to prevent damage to the probe and lead wires (see Figure 5 on page 22 ).
6. Make sure the probe is connected to the digital thermometer.
7. Slide the heated cover forward and pull the lever down.
IMPORTANT!
Seat the probe properly and close the heated cover carefully. If
the probe wire is crushed when you close the heated cover, the probe can be
damaged.
8. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.