1. Using a grease pencil or similar marking device, place a reference mark on the capillary tubing of
each absorption pipette. The reference mark should be located about half way between the
pipette bodies and the stopcock. The exact position of these marks is not critical. The marks are
only a reference point to ensure that the manifold volume is the same during each reading of the
burette volume. (Figure 4)
2. Attach the Tedlar expansion bag to the top cross bar using plastic wire ties or a wire. Suspend
the bag from the crossbar using the brass eyelets so that the metal hose barbs hang close to the
glass hose barbs on the reagent bottles. Using 2 inch pieces of rubber tubing, attach the hose
barbs on the reagent bottles to the three-section Tedlar bag hose barbs. (Figure 5)
Figure 4 Marking Pipette Capillaries
Figure 5 Top View of Orsat with Tedlar Bag attached to Pipette Bottles
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