Antec Scientific VT-03, User Manual, Page: 25 / 36

CHAPTER 3 Installation 25
Fig. 19. Mounting of the fused silica connector in the VT-03 micro flow cell.
The capillary connector is not necessary with a 1 mm (or larger ID) column.
In that case use narrow bore PEEK tubing (

100 µm ID), and install as
described on page 16 (ISAAC REF) or on page 18 (salt bridge REF).
To prevent any damage to the flow cell the following steps should be carried
out (Fig. 19):
1. Connect the FS capillary to the pump (by-passing the column) and
switch on the pump to check that the capillary is open. Liquid droplets
must come out. Otherwise replace with new piece of 100 um FS tubing.
Note that both endings of the supplied FS capillary are cut and polished!
2. If the capillary is open, first switch off the pump and wait till the
pressure is zero . Disconnect the capillary from the pump.
3. Insert the fused silica capillary into the tightly fitting Teflon sleeve
supplied.
4. Protrude both through our factory supplied fingertight fitting. Use only our
factory supplied fingertights in the flow cell, others may cause serious
damage!
5. Mount this combination carefully in the injection block (Fig. 19).
6. Let the fused silica slightly (< 0.5 mm) protrude through the injection
hole.
7. Clean the factory supplied glass mounting plate from particles.