Ankom 2000, Operator'S Manual

Page: 39 / 44

Operator’s Manual
Rev 05/12/21
pg. 39
Calculations
% NDF (as-received basis) = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of bag with fiber
after extraction process
C
1
= Blank bag correction (running
average of final oven-dried
weight divided by original blank
bag weight)
NDF Procedure (continued)
11. Open the Vessel Lid and insert the Bag Suspender with bags
into the Vessel and place the Bag Suspender weight on top of
the empty 9
th
tray to keep the Bag Suspender submerged.
NOTE: If you do not want to use the Cubitainers to
automatically add your solutions, you can manually fill the
vessel for each procedure. First make sure that the hoses to the
Cubitainers are disconnected. Follow steps 12a and 12b
below. Then press START on the Keypad and immediately
pour 2L of solution directly into the vessel. Then add 20g of
Na
2
SO
3
and 4.0ml of alpha-amylase. Please note that the
solution must cover the level sensor in order for the
instrument to start its operation. Once agitation begins, close
the Vessel Lid and follow the rest of the procedure from step
13 to the end.
12. Follow the instructions on the ANKOM
2000
display:
a. Select NDF. ( Wait to close the Vessel Lid.)
b. Confirm hot water is on (>70°C).
c. Press START.
d. After the ND solution has been automatically
inserted and agitation begins, manually add 20g of
Na
2
SO
3
and 4.0ml of alpha-amylase.
e. Close the Vessel Lid.
13. Attach the Amylase Dispenser Assembly to Port B on the
instrument. Add 8.0ml of alpha-amylase and enough water to
fill the dispenser. The ANKOM
2000
will automatically add the
amylase solution to the first and second rinse.
14. When the NDF extraction and rinsing procedures are
complete, open the Vessel Lid and remove the filter bags.
Gently press out excess water from the bags. Place bags in a
250ml beaker and add enough acetone to cover bags and soak
for 3-5 minutes.
15. Remove the filter bags from the acetone and place them on a
wire screen to air-dry. Completely dry in an oven at 102 ±
2°C. (In most ovens the filter bags will be completely dry
within 2-4 hours.) Do not place bags in the oven until the
acetone has completely evaporated.
NOTE: When running a lignin procedure or a sequential
(NDF/ADF or NDF/ADF/Lignin) with the F57 Filter Bag it is
important not to dry the bags overnight after the NDF or ADF
procedure. A drying timeframe of 2-4 hours at 100°C to
105°C is sufficient to thoroughly dry the bags after each
procedure. Extended drying times or too high a temperature
can compromise the bag’s filtration media. In addition, be
sure to check the water of the fourth rinse during the ADF
procedure to ensure all the sulfuric acid has been removed
from the bags. If litmus paper shows the presence of acid
during the fourth hot water rinse, repeat until neutral.
16. Remove the filter bags from the oven and immediately place
them directly into a collapsible desiccant pouch and flatten to
remove any air. Cool to ambient temperature and weigh the
filter bags (W
3
). NOTE: Do not use a conventional countertop
or cabinet desiccator.