Analytik Jena 850-203-001-0010 Instructions For Use Manual Download Page 10 | Manualshive

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LOOXSTER

®

Enrichment Kit 11/2013

10

Literature

[1]

Chao Xu, Chuanbing Bian, Robert Lam, Aiping Dong & Jinrong Min. The structural
basis for selective binding of non-methylated CpG islands by the CFP1 CXXC
domain. Nat. Commun. 2011;2:227.

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Summary of Contents for 850-203-001-0010

Page 1: ...Analytik Jena AG Life Science Konrad Zuse Straße 1 07745 Jena Deutschland www bio analytik jena de LOOXSTER Enrichment Kit Instructions for use english For research use only ...

Page 2: ...ment Kit 11 2013 2 850 203 001 0010 10 Manufacturer Analytik Jena AG Konrad Zuse Straße 1 07745 Jena Germany Phone 49 0 36 41 77 94 00 Fax 49 0 36 41 77 76 77 76 www bio analytik jena com lifescience analytik jena com ...

Page 3: ...ent of bacterial and fungal DNA in a DNA isolate containing predominant amounts of eukaryotic DNA cells tissue or body fluids LOOXSTER Enrichment is intended for molecular biology applications The product is not intended for the diagnosis prevention or treatment of a disease Komponent 10 Isolations Symbol LOOXSTER Magnetic Particles 5 screwed cap tubes with lyophilisate LMP LOOXSTER Binding Buffer...

Page 4: ... and precautionary Statements H225 H319 H336 P210 P233 P305 P351 P338 Desalting Wash Buffer DWB contains ethanol flammable Hazard and precautionary Statements H225 P210 H225 Highly flammable liquid and vapour H302 Harmful if swallowed H315 Causes skin irritation H319 Causes serious eye irritation H336 May cause drowsiness or dizziness P210 Keep away from heat hot surfaces sparks open flames and ot...

Page 5: ...he LOOXSTER protein forms a stable complex with non methylated CpG dinucleotides Dissociation constant Kd 2 7µM1 Unbound methylated DNA can be removed from the sample by means of stringent washing step Subsequently the protein DNA complex is solved in presence of an elution buffer and the enriched bacterial or fungal DNA can be harvested from the LOOXSTER particles After performing a final solid p...

Page 6: ... for the total DNA content present in a single sample applied to LOOXSTER Enrichment The DNA binding capacity of 175 µl of reconstituted LOOXSTER Magnetic Particles is 3 0 µg Essential Instrumentation The LOOXSTER Enrichment Kit contains coated paramagnetic particles Processing of paramagnetic particles requires a strong magnetic separator adapted to 1 5 ml and 2 0 ml reaction tubes We recommend M...

Page 7: ...only one LOOXSTER enrichment application is carried out the remaining volume of LOOXSTER Magnetic Particles can be stored at 4 8 C for at least one week without affecting its performance 1 Open Screwed cap tube with lyophilized LOOXSTER Magnetic Particles LMP and add 400 µl of Water W Reconstitute LOOXSTER Magnetic Particles LMP for 30 min using the Thermomixer 25 C 600 rpm Resuspend the reconstit...

Page 8: ...erting the tube several times Place the tube in the Thermomixer and incubate preparation for 3 min at 25 C and 800 rpm LWB 3 Place preparation in the magnetic separator and wait for 30 60 sec Ensure complete separation of LOOXSTER Magnetic Particles LMP by carefully inverting the Tube 2 0 ml TUB2 several times while being placed in the magnetic separator Remove the particle free supernatant from t...

Page 9: ... 13 000 16 000 x g DWB 3 Remove flow through and place Desalting Spin Column DSC into the same Collection Tube CT Apply 300 µl of Desalting Wash Buffer DWB to the Desalting Spin Column DSC again Close lid and centrifuge Spin Column DSC for 30 sec at 13 000 16 000 x g DWB 4 Remove flow through and place Desalting Spin Column DSC into a new Collection Tube CT Centrifuge Spin Column DSC for 30 sec at...

Page 10: ...richment Kit 11 2013 10 Literature 1 Chao Xu Chuanbing Bian Robert Lam Aiping Dong Jinrong Min The structural basis for selective binding of non methylated CpG islands by the CFP1 CXXC domain Nat Commun 2011 2 227 ...

Page 11: ...203 001 0010 10 Technical Support If you have any questions or experience any difficulties regarding LOOXSTER please do not hesitate to contact us for technical support You can contact us by email phone fax or post Analytik Jena AG Life Science Konrad Zuse Straße 1 07745 Jena Germany Tel 49 0 3641 77 97 00 Fax 49 0 3641 77 76 97 00 E Mail lifescience analytik jena de ...

Page 12: ...per l uso Consulte las instrucciones de uso Manufacturer Hersteller Fabricant Fabbricante Productor For research use only Ausschließlich zu Forschungszwecken Seulement pour rechérche Solo per uso di Ricerca Sólo para uso en investigación Concentrate Konzentrat Concentré Concentrato Concentrar Lyophilized Lyophilisat Lyophilisé Liofilizzato Liofilizado Keep away from heat or direct sunlight Vor Hit...

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