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B110 User Manual 

Page 10 

 

 

2.3 Locating the Specimen and Focusing 

     

 

With the specimen mounted on the stage, and the illuminator turned on, use the stage’s X-Y control knobs under the 
right side of the stage to adjust the position of the specimen. The larger knob will move the specimen forward and 
backward, while the smaller knob will move the specimen left and right. Compound microscopes produce inverted 
images due to the nature of the optical system. Sliding the specimen towards the front of the microscope will result in 
the viewed image moving in the opposite direction. Use the knobs until the specimen appears to be centered under the 
objective lens. This will be the starting point.  

Observation should typically begin at the lowest magnification. This provides the broadest view of the specimen. Rotate 
the objective turret to set the lowest magnification lens in place. The condenser is typically designed with a range of 
aperture values ranging from approximately 0.10 to 1.25. If using a 4X objective lens with a 0.10 aperture, adjust the 
condenser’s aperture using the lever on the outside of the condenser to reduce the size of the aperture. This will 
maximize the focus depth. When higher magnifications are used, the aperture should be adjusted more open to better 
match the objective lens’ aperture.  

Look through the eyepieces, adjusting the interpupillary adjusters as needed to achieve proper alignment. Both eyes 
should see the same image with a bright, round background. If the image appears offset or obscured, you should contin-
ue to adjust the interpupillary distance until the image appears correct. The left ocular tube also has a dioptric adjuster 
which can be used if the eyes do not focus the same. If the eyepieces are properly aligned, but the light-source appears 
offset, use the two centering screws attached to the condenser holder to adjust the condenser’s position until it is cen-
tered.  

The stage should be set to a low position when mounting the specimen. Gradually raise the stage using the coarse-focus 
knob. If you have already performed the height-limit adjustment, the specimen should come into focus near the maxi-
mum height. Once you begin to see shapes through the eyepieces, use the fine-focus knobs to adjust the focus until you 
can see details with clarity. Use the X-Y control knobs to reposition the specimen until the area of interest is in view. You 
can increase magnification as needed by rotating the objective turret. When doing so, it is advisable to watch the lens as 
it is set in place to ensure it will not collide with the specimen. Slight adjustments to focus should be made at each mag-
nification, as well as to the condenser’s aperture and the illuminator’s brightness.

 

 

 

Summary of Contents for B110C

Page 1: ...B110C AmScope 40X 1000X dual LED Ergonomic Lab Binocular Compound Microscope with 3D Two Layer Mechanical Stage Product Manual ...

Page 2: ...avior Do not use flammable liquids or gases Electric instruments can ignite flammable substances which could result in an explosion or fire Do not use in a wet environment Electrical components of the instrument can discharge when exposed to water potentially resulting in damage to the instrument or injury to yourself or others Do not dismantle Dismantling can result in damage to the instrument an...

Page 3: ...B110 User Manual Page 3 ...

Page 4: ...4 What s In The Box The B110C standard outfit includes One microscope Three objective lenses 4X 10X 40X Two pairs of eyepieces 10X 25X One dust cover One AC power cord Additional items may be included depending on the model ...

Page 5: ...rted into the ocular tubes or alternatively an anti static cover can be placed over the head to prevent dust contamination To use this instrument for standard observation eyepieces must be attached to produce a viewable image Insert a pair of eyepieces into the ocular tubes Ensure that the eyepieces have matching magnification as some models contain multiple pairs of eyepieces with different magni...

Page 6: ...s are typically mounted in order from 4X to 40X but can be interchanged as desired This microscope is compatible with DIN standard objective lenses which allows aftermarket lenses to be used as needed These lenses must comply with the following DIN specifications 20 32mm RMS mounting thread 160mm mechanical tube length 45mm parfocal distance DIN stands for Deutsches Institut für Normung which is a...

Page 7: ...cimen mounted on the stage slowly raise the stage using the coarse focus knob until the specimen is a few millimeters from the lens Use the fine focus knob to continue raising the stage until the specimen barely comes in contact with the lens The height adjustment screw should be tightened to set the stopping position If the specimen was too far from the lens and could not make contact loosen the ...

Page 8: ...r in wet environments Attach the provided power cable ensuring that the cable is fully inserted with a firm connection Plug the cable into an AC outlet then set the power switch to the on position to activate the built in illumination This microscope can also function with three AAA batteries Remove the bottom cover and insert batteries in the correct orientation Batteries are not included ...

Page 9: ...ong edge is flush against the back edge of the tem plate Gently releasing the caliper will secure the slide in place 2 2 Illumination The microscope s power switch is located on the rear of the body Turn on the microscope s illumination by pressing the power switch to the on position As magnification increases more light will be necessary to view the specimen Use the wheel on the right side of the...

Page 10: ...re open to better match the objective lens aperture Look through the eyepieces adjusting the interpupillary adjusters as needed to achieve proper alignment Both eyes should see the same image with a bright round background If the image appears offset or obscured you should contin ue to adjust the interpupillary distance until the image appears correct The left ocular tube also has a dioptric adjus...

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