AmScope 490 Series Operator'S Manual Download Page 1

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OPERATOR’S

MANUAL

(Please Read This Manual Before Using the

Microscope

)

490 Series

Compound Microscope

Summary of Contents for 490 Series

Page 1: ...OPERATOR S MANUAL Please Read This Manual Before Using the Microscope 490 Series Compound Microscope ...

Page 2: ...n this document and all rights thereto are the sole and exclusive property of AmScope and are not to be copied used or disclosed to anyone in whole or in part without the express written permission of AmScope AmScope assumes no liability for any damages incurred directly or indirectly from any errors omissions or discrepancies between the use of this microscope and the information contained in thi...

Page 3: ...ser Lens Optional Accessory 21 2 19 Using a Phase Contrast Kit Optional Accessory 24 2 20 Using a Simple Phase Contrast Kit Optional Accessory 28 2 21 Using a Turret Phase Contrast Kit Optional Accessory 33 2 22 Microscope Maintenance 38 2 23 Darkfield Condenser Kit Phase Contrast Kit Maintenance 39 3 GENERAL SPECIFICATIONS 40 3 1 General Model Comparison 40 3 2 Objectives 41 3 3 Eyepieces 41 3 4 ...

Page 4: ...keep it on hand for future reference If you have additional questions or need assistance please send us an email at info AmScope com Please include the microscope model number in your email so that we can identify your model and provide immediate help Precautions 1 As the microscope is a precision instrument always handle it with care avoiding impact or abrupt movement during transportation Do not...

Page 5: ...490 Series Microscope AmScope Proprietary Page 5 of 64 1 Microscope Parts Compound Microscope ...

Page 6: ...scope Body Ø One Binocular B490 or One Trinocular T490 Rotating Gemel Head Ø One Adjustable Trinocular Photo Port for T490 only Ø Four Objectives 4x 10x 40x 100x Ø Pair of 10X Widefield Eyepieces Ø Pair of 16X Widefield Eyepieces for A models only Ø Pair of 20X Widefield Eyepieces for B models only Ø Pair of 25X Widefield Eyepieces for C models only Ø Three Color Filters Blue Green Yellow for non ...

Page 7: ...the nosepiece 2 2 2 Loosen the Head Lock Screw Loosen the metal knob located directly above the nosepiece the head lock screw completely 2 2 3 Install the Microscope Head Carefully take the microscope head out of the plastic bag making sure not to touch glass Place the bottom side of the microscope head flat circular side down into the circular opening on top of the arm just above the nosepiece an...

Page 8: ...ort cap from the top side of the microscope head Screw in the Trinocular port If your camera has a c mount insert the c mount adapter onto the Trinocular port If your camera does not have a c mount you can skip this step 2 2 5 Insert Eyepieces Remove the eyetube caps Place the desired eyepieces into the empty eye ocular tubes Be sure to avoid touching the lenses ...

Page 9: ...to the microscope nosepiece Start with the lowest magnification and in order of magnification insert each lens without touching the glass 2 2 7 Plug it in turn it on and adjust the dimmer if more light is needed Plug in the microscope and turn it on If no light emerges from the light source adjust the dimmer knob on the side of the base ...

Page 10: ...ou are looking over the stage in reverse position you are looking at the stage Once you ve chosen your preferred microscope head position lock the head lock screw 2 3 2 Focus the distance between your eyes The interpupillary distance the distance between your eye pupils With both eyes open look into the eyepieces Adjust the interpupillary distance by holding the eyetubes and rotating the tubes eit...

Page 11: ... metal slide holders clips of the mechanical stage 2 4 3 Center the Specimen Using the mechanical stage controls center the slide over the stage opening lining it up with the light and the objective lens The top ring moves the stage forward and backward The bottom ring moves the stage from left to right 2 4 4 Adjust the dimmer if more light is needed To adjust the illumination slowly turn the dimm...

Page 12: ... right eyetube Or if you have two diopters select either eyepiece 2 Close your other eye 3 Focus the image using the coarse focusing knob Adjust the height of the stage until the sample comes into clear focus Note You can loosen the limit stop knob located on the inside of the coarse focusing knob on the left side of the microscope in order to give yourself the full range of motion 2 5 4 Focus you...

Page 13: ... specimen s image seen through the magnified objective lens is very large The reduction lens renders the image in a size compatible with the smaller size of the digital camera s sensor 1 If your camera has a 23mm mounting size you do not need the C mount adapter If installed remove the C mount from the top of the Trinocular port by loosening the screw 2 Place the camera mounted on a reduction lens...

Page 14: ... 4 To reset it unlock the stage and reset the stage to the new height If no limit is desired simply unlock the stop limit 2 9 Adjusting Focus Tension 1 To adjust the tension of the focusing knobs first locate the black ridged tension knob on the inside of the coarse focusing knob 2 To decrease tension rotate the adjustment forward towards the stage counterclockwise To increase rotate away from the...

Page 15: ...nt slider located directly under the stage 2 12 Changing a Filter 1 At the bottom of the condenser lens there is a small ring that slides out Swivel the ring as far out as possible 2 Insert the preferred colored glass filter 3 Slide the ring back into place Note The filter holder is placed in from the factory in a manner in which it swings out and hits the arm of the microscope backwards If this h...

Page 16: ...reaking 2 Carefully turn the microscope on its side exposing the base 3 Loosen the door screw on the bottom of the microscope and pull the door open 4 Remove the old bulb and replace with a new one 5 Close door and tighten the screws of the door 2 14 Changing the Fuse Microscope Base 1 Turn off and unplug the microscope 2 Be sure to remove the eyepieces from the unit before turning the microscope ...

Page 17: ...ssible Increase light intensity once the specimen is clearly illuminated 3 Move the objectives out of the way to enable access to the slide Place a drop of immersion oil directly onto the slide s cover slip not directly on the specimen a cover slip MUST be used 4 Release the stage stop limit 5 Lower your oil immersion lens 100X objective into position so that the front lens is immersed in the oil ...

Page 18: ... to make adjustments 7 Re engage the stage stop limit 8 When the specimen is in focus adjust the iris to open just larger than the condenser s diameter 9 After using the oil immersion lens you will need to clean the lens and specimen before the oil dries Note Please be careful when using the stage stop limit The stage limit stop is designed to prevent impact between objective and slide so when it ...

Page 19: ...he 40X and 100X objective lenses with numeric apertures up to 1 25 or less 2 16 1 Installing a Darkfield Condenser Lens 1 Use the condenser lens adjustment knob to lower the condenser so you have easy access to the condenser knob 2 Loosen the condenser lock screw and replace the condenser lens 3 Install the darkfield condenser lens and tighten the condenser lock thumb screw on the condenser holder...

Page 20: ...disc is slightly smaller than the entire image circle leaving a ring of light 4 Turn the condenser s centering screws to move the dark spot to the center 5 Replace the eyepieces 6 Raise the condenser to the top of its range just short of the slide on the stage The is the optimal position for darkfield viewing but can be adjusted later if necessary 7 Place a slide on the stage and focus Adjust the ...

Page 21: ...the condenser until the opaque disc is slightly smaller than the entire image circle leaving a ring of light Note The dark spot has a fuzzy edge and will become larger when the condenser gets closers to the objective The distance between the top of the condenser and the objective is about 8mm when the dark spot shows in the field of view 4 Turn the condenser s centering screws to move the dark spo...

Page 22: ...ce the slide on the stage 3 Raise the condenser and let the oil drop contact the underside of the slide If air bubbles exist in the oil clean the oil from the condenser lens and bottom of slide and repeat the procedures 4 Apply a drop of immersion oil to the slide s coverslip 5 Carefully raise the stage until the oil makes contact with the objective lens 6 Adjust the iris ring to get proper bright...

Page 23: ...the oil If you want to use a cleaner you can use cigarette lighter fluid to safely remove the oil as it will break it down Do not use alcohol based solvents as they dissolve the cement used to in assembly of the lens thus breaking your objective 3 The specimen itself can be discarded or if desired you could clean it up and store it for later use The specimen may also be left with the oil on it as ...

Page 24: ...ecimen which offers little contrast in normal bright field conditions Light waves have several measurable components the most common of which are length frequency and amplitude While length and frequency translate into the color of light we see amplitude translates into brightness One component that we do not perceive is phase which can be described as how much of the wave s cycle has elapsed at a...

Page 25: ...ht wave s length amplitude frequency and phase There are shifts in phase due to changes in light s speed As the speed of light changes when hitting or missing obstacles like a specimen the rays are bent or refracted The amount of refraction when a light wave hits an obstacle is measured as its refractive index It s this refractive index which creates the variations in phase as light passes through...

Page 26: ...nnulus passes more centrally through the specimen in the objective lens As the light collectively passes through the objective lens it is further segregated by the phase ring The phase ring shifts the phase of the surround light either positively or negatively so the waves are in phase Since human sight is incapable of perceiving light phase the aim of phase contrast is to translate the disparitie...

Page 27: ...ntrast objective lenses three phase contrast condenser lenses and a centering telescope AmScope s PCT Turret Phase Contrast Kit includes four phase contrast objective lenses a condenser turret and a centering telescope The turret has built in annuli designed to work with each of the lenses as well as a brightfield iris ...

Page 28: ...tems You may need to use the key taped onto the box Check carefully to ensure that all parts and accessories are intact Note You must match finite tube length kits to finite tube length microscopes and infinity corrected units to infinity corrected microscopes for optimal results 2 Check the packing list to ensure that you re received all the items All units should come with Ø 10X 40X 100X Phase C...

Page 29: ...ndenser is installed 4 Focus the specimen using the 10X objective Adjust the iris ring on the condenser so the light field is just larger than the field of view in the microscope The condenser itself should be moved to the normal height which is close to the bottom of the stage of the microscope the top of the range of the condenser 2 20 3 Installing a Simple Phase Contrast Condenser 1 Loosen the ...

Page 30: ...ogether For example the 10X phase contrast objective must work with the 10X condenser ring plate 2 20 4 Calibrating the Phase Contrast Objective Lens and Condenser Lens 1 Remove one eyepiece from the microscope eyetube and insert the centering telescope 2 Turn the microscope s light on and look through the telescope lens 3 While looking through the eyepiece extend the eyepiece by rotating the top ...

Page 31: ... inside the other adjust the centering screws on the condenser ring plate until the two rings center in parallel The annulus bright ring of light should overlap the dark grey ring which is the objective s phase ring 6 Place the green filter in the filter holder or directly onto the center of the base lens of your microscope where the light emerges from Remove the green filter if doing darkfield mi...

Page 32: ...trast observation the same way as a normal brightfield microscope Note When changing to another phase contrast objective and corresponding condenser ring plate the focusing and centering of bright ring and dark ring should be repeated following the procedures above Tips 1 Make the illumination as bright as possible 2 The thinner the specimen the better the image ...

Page 33: ... may need to use the key taped onto the box Check carefully to ensure that all parts and accessories are intact Note You must match finite tube length kits to finite tube length microscopes and infinity corrected units to infinity corrected microscopes for optimal results 2 Check the packing list to ensure that you re received all the items All units should come with Ø Turret with 10X 20X 40X 100X...

Page 34: ...the Turret Phase Contrast Kit 1 Rotate the microscope s nosepiece to the 10X phase contrast objective lens Objective lenses designed for phase contrast will be marked Ph this denotes the objective lens has a built in phase ring Note There are 4 phase contrast objectives 10X 20X 40X and 100X and there are 5 settings on the condenser s turret ring 10 20 40 100 and B The numbers on the turret ring mu...

Page 35: ... setting 4 Once the specimen is in focus with the brightfield setting rotate the condenser s turret ring so it is set to 10 2 21 4 Calibrating the Turret Phase Contrast Objectives and Condenser Lenses The turret has a built in annulus for each objective magnification Once the turret and objective lens are set with matching numbers they will need to be aligned You will need to do this again each ti...

Page 36: ...ng of light should overlap the dark grey ring which is the objective s phase ring 6 If the bright ring is still obscure raise or lower the condenser by adjusting the condenser focusing knob or the microscope focusing knob if necessary until the bright ring is in focus and the dark ring is visible 7 Place the green filter in the filter holder or directly onto the center of the base lens of your mic...

Page 37: ...trast observation the same way as a normal brightfield microscope Note When changing to another phase contrast objective and corresponding condenser ring plate the focusing and centering of bright ring and dark ring should be repeated following the procedures above Tips 1 Make the illumination as bright as possible 2 The thinner the specimen the better the image ...

Page 38: ... keep it in a dry and clean place in order to prevent rust 2 22 4 Keep Glass Clean All glass surfaces must always be kept clean Oil and dirt from your fingerprints will obstruct your view Fine dust on the optical surface should be blown off using a hand blower or gently wiped off with a soft lens tissue 2 22 5 Use an optical lens cleaner Carefully wipe off oil or fingerprints on lens surfaces usin...

Page 39: ...use organic solvents for cleansing 2 23 4 Keep Lens Clean The outer surface of the optics should be inspected and cleaned periodically using an air stream from an air bulb If dirt remains on the optical surface use a soft cloth or cotton swab dampened with a lens cleaning solution available at the camera stores All optical lenses should be swabbed using a circular motion A small amount of absorben...

Page 40: ... if C DIN Achromatic Objectives 4X 0 10 10X 0 25 40X spring 0 65 100X spring oil 1 25 30 Degree Viewing Head Binocular Sliding 360 Degree Swiveling Trinocular Sliding 360 Degree Swiveling Trinocular Port C Mount Adjustable Photo port 23mm Adjustable Photo Port Simultaneous Viewing Focusing Nosepiece Coaxial Focusing System Quadruple Nosepiece Stage 130mm x 140mm Mechanical Stage Movement Range 50m...

Page 41: ... A60X 60X 0 85 Air Dark Blue A100X 100X 1 25 Cedar Oil White Plan Objective 195mm PA4X Plan 4X 0 10 Air Red PA10X Plan 10X 0 25 Air Yellow PA40X Plan 40X 0 65 Air Light Blue PA100X Plan 100X 1 25 Cedar Oil White 3 3 Eyepieces Eyepieces Magnification 5X 10X 15X 16X 20X 25X Field of View Φ18 Φ18 Φ13 Φ15 Φ11 Φ9 3 4 Darkfield Condensers Specifications Dry Darkfield Condenser Oil Darkfield Condenser Mo...

Page 42: ...th built in phase plate spring Plan phase contrast 40X 0 65 160 0 17 Achromatic 100X 1 25 160 0 17 with built in phase plate spring oil Plan phase contrast 100X 1 25 160 0 17 Oil Numerical Aperture N A Rating 1 25 1 25 Turret Ring Plates 1 For 10X phase contrast objective 10 for 10X phase contrast objective 20 for 20X phase contrast objective 1 For 40X phase contrast objective 40 for 40X phase con...

Page 43: ...n or an LED system 1 100V 120V power supply 100V 120V 10 50 60Hz 2 220V 240V power supply 220V 240V 10 50 60Hz All units come standard as 110V units unless an upgrade to a 220V system is requested Upgrade fee is dependent on which unit is purchased 4 2 Microscope Parameters Magnification A Model 40x 1600x B Model 40x 2000x C Model 40x 2500x Field of View Φ0 8mm Φ4 5mm Mechanical Tube Length 160mm ...

Page 44: ...e visit www amscope com and search with SKU for more information 5 1 Eyepieces 5X Eyepieces 20X Eyepieces 25X Eyepieces SKU EP5X23 SKU EP20X23 SKU EP25X23 10X w Pointer 10X w Reticle SKU EP10X23P SKU EP10X23R 5 2 Darkfield Condensers Dry Darkfield Condenser Oil Darkfield Condenser SKU DK DRY100 SKU DK OIL100 ...

Page 45: ...y Page 45 of 64 5 3 Objective Lenses Achromatic 2X 5X 20X 60X SKU A2X SKU A5X SKU A20X SKU A60X Plan Achromatic Plan 4X Plan 10X Plan 40X Plan 100X SKU PA4X SKU PA10X SKU PA40X SKU PA100X 5 4 Phase Contrast Kits Simple Turret SKU PCS SKU PCT ...

Page 46: ... 5 Mega Pixel 8 Mega Pixel 9 Mega Pixel 10 Mega Pixel SKU SKU SKU SKU SKU SKU SKU MU035 MU130 MU300 MU500 MU800 MU900 MU1000 Calibration Micrometer Calibrate the camera software for on screen measurements SKU MR400 Charge Coupled Device Television CCD Digital VGA Trinocular Only CCD TV Video Trinocular Only 1920x1080 Full HD HDMI Trinocular Only SKU CCD MT SKU CCD NP SKU HD1080 To view live displa...

Page 47: ...s 6V 20W Halogen Bulb 6V 30W Halogen Bulb Fuses For Microscopes SKU BH 6V20W SKU BH 6V30W SKU FS M T1AL 220V 5 7 Cases and Bags Aluminum Case Hard Wood Case Vinyl Bag SKU AC B400 SKU WC B400 SKU CB B490 5 8 Stage Warmer Stage Warmer TCS 100 Microscope Temperature Control Stage Slide Warmer ...

Page 48: ...2 SKU BS 144P 200S 22 SKU BS 300P 300S 5 10 Cleaners Sparkle Microscope 3 in 1 Professional 3 in 1 Cleaning Kit Optical Lens Cleaner Cleaning Kit for for Microscopes SKU CLS Microscopes Cameras Cameras and and Laptops LCD Screens SKU CK I SKU CK II 5 11 Books Complete Book of the Microscope The Ultimate Guide to Your Microscope The World of the Microscope What to look for Ages 8 Adult Science and ...

Page 49: ...oughly clean the objective lens with lens cleaner or a lint free non abrasive cloth No immersion oil is used with the 100X objective Use immersion oil for better clarity and resolution Used wrong oil Use standard cedar wood oil The aperture is not open to an appropriate diameter Adjust the aperture to have the light just larger than the size of the condenser Stains dust or dirt has accumulated on ...

Page 50: ...mechanical stage screws to better secure the stage The objective touches the cover slip The cover slip is not standard sized Replace the cover slip with the appropriate 0 17mm thickness slip The limit stop is set too high or not engaged Be careful to avoid contact between objective and the slide when the limit stop is not engaged unless using the 100X objective with oil To re engage focus the samp...

Page 51: ...place the bulb This unit uses our BH 6V20W with our 20w unit or our BH 6V30W for the 30W unit The fuse burned out Replace with fuse on the bottom of the microscope The fuse burns out frequently The voltage is too high Use the correct power supply 110v if 110v unit 220v if 220v unit or get a voltage adapter to convert to the proper electrical system The bulb burns out frequently The voltage is too ...

Page 52: ...transparent specimens like slides and biological subjects Compound microscopes show a two dimensional image of the specimen usually reversed and upside down The common magnification range of our compound microscopes is between 40X 1000X could be up to 2500X You need a minimum of 400X to study cell structure Stereo microscopes are best for larger specimens you cannot see through Stereo microscopes ...

Page 53: ...oving the eyepieces toward or away from each other The rotating feature allows for either forward or reverse view depending on whether you d rather look at the specimen or over it C Mount C Mounts are adapters with a standard thread for mounting a lens to a camera C Mounts fit onto a Trinocular port Condenser Lens A condenser lens concentrates light on a specimen and increases the resolution An ad...

Page 54: ...eam of light illuminates the objective lens Darkfield condenser lenses block the center of the beam of light to produce a hollow cone of light Light does not enter the objective lens directly The specimen scatters light which then enters the objective lens Brightfield Darkfield Dimmer Dimmers control the amount of light escaping from the base lens DIN JIS DIN is the Deutsches Institut für Normung ...

Page 55: ...ace them by swapping them out of the eyetubes The eyepieces magnify the specimen further from the first magnification through the objective lens Each eyepiece is marked with 2 numbers The first number is the magnification and the second is the field of view For example an eyepiece marked WF10 20 has a magnification of 10X and a field of view of 20mm Eyetubes Eyetubes are also called ocular tubes E...

Page 56: ...ob Together working distance and field of view determine how much of the specimen you see and how closely Filters Optical filters selectively transmit light of different wavelengths Blue filters compensate for low light levels in a brightfield application Like dry layer blood analysis With low light you see a yellowing effect The blue filter brings the light back to a more natural white light Gree...

Page 57: ...00X objective lens Fine Focus The outer knob used to fine tune the focus of a specimen in conjunction with the coarse focus Head Lock Screw The head lock screw secures the microscope head to the microscope Loosen the head to rotate between forward and reverse mounting Tighten the screw to lock the microscope head position into place Head Orientation Microscope head orientation is for user preferen...

Page 58: ...ws you to look at a specimen with a camera without removing an eyepiece Multi head Multi head microscopes are specially designed for three person observation Ideal for teaching and or training purposes It has a binocular head and two monocular heads Simul Focal Simul Focal heads mitigate the amount of re focusing needed when you change your view from the eyepieces to the monitor Perfect for lectur...

Page 59: ...ds adjust the interpupillary distance by rotating the eyetubes around a central axis A Siedentopf head changes the interpupillary distance without changing the tube distance Jentsch heads adjust the interpupillary distance by moving the eyetubes closer together or farther apart in a linear fashion The tube length changes when changing interpupillary distance so the user compensates by adjusting th...

Page 60: ...ungsten Tungsten light bulbs are the most economical providing a reliable source of light They burn hotter than the other bulbs but are the best option for certain microscopes Fluorescent Fluorescent bulbs burn cooler and brighter than tungsten bulbs The bulbs are more expensive than tungsten but last longer LED LED light bulbs burn the coolest of all the bulbs and last the longest The bulbs are a...

Page 61: ...wer of two lenses multiplied together E g 10X Eyepieces x 4X Objective 40X Total Magnification Mechanical Stage A Mechanical Stage is a flat mechanism that sits on top of the stage and allows the viewer to move a specimen small distances a task that is otherwise difficult at higher magnifications Most mechanical stages are equipped with an X and Y axis so the viewer can see how far the slide has m...

Page 62: ... specimen stays centered Most compound microscopes are parcentered Parfocal When changing objectives the image of the specimen stays in focus without needing to adjust the coarse focusing knob Not all compound microscopes are parfocal Phase Contrast Many biological specimens are virtually transparent when viewed with a typical compound microscope Phase contrast microscopy is a method that collects...

Page 63: ...nser Assembly For phase contrast kits without a turret the condenser assembly is the mount for the condenser lenses Phase Contrast Objective A specific objective with a ring printed on it that is used for phase contrast The objective is the bottom lens that determines the resolution of the image in the microscope and is one of the two lenses that compound to make total magnification Phase Contrast...

Page 64: ...in centering the light in phase contrast microscopy Tension Knob The large innermost ring on the focusing knob Adjusts the tension of the focusing knobs Trinocular Port A trinocular microscope has three viewing ports There are two eyepieces and a third port for photography and video The trinocular port is a 23mm adjustable photo port and uses a C Mount adapter ...

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