Amersham Biosciences Hoefer DALT, User Manual, Page: 45 / 70

Hoefer DALT System Recipes
Amersham Biosciences
41
Recipes
DALT Acrylamide Stock (30.8 %T)
WARNING Acrylamide is a neurotoxin. Always use mechanical pipettes and wear
gloves when working with acrylamide solutions.
1.5 M Tris-Cl, pH 8.8
10% SDS
10% Ammonium Persulfate
Final Conc. Amount
Acrylamide (best affordable grade, MW 71.08) 30% 900 g
Bis (N, N’ methylenebis-acrylamide, purest grade, FW 154.17) 0.8% 24 g
Water (purest available) up to 3000 ml
May need filtration. Weigh acrylamide and bis under a hood; avoid contact with dust. Filter
and store at 4 °C.
Final Conc. Amount
Tris (MW 121.14) 1.5 M 545 g
6N HCl to pH 8.8 about 150 ml
Water to 3000 ml
Adjust to pH 8.8, store at 4 °C.
Final Conc. Amount
Sodium dodecylsulfate (MW 288.38) 10% 10 g
Water up to 100 ml
Store at room temperature.
Final Conc. Amount
Ammonium persulfate (MW 228.2) 10% 2 g
Water up to 20 ml
Make fresh.