Amersham Biosciences Hoefer DALT, User Manual, Page: 19 / 70

Hoefer DALT System Casting Homogeneous (Non-Gradient) Gels
Amersham Biosciences
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10. Once the pouring is complete, remove the feed tube from the balance chamber
grommet.
As soon as the feed tube is removed, the dense blue displacing solution flows down
the connecting tube to the unit, fills the V-well and the sloped trough at the bottom
of the caster. If the V-well is not completely filled and the level of gel in the casettes
is more than 1 cm below the top of the cassettes, you may add up to 50 ml more
displacing solution to the balance chamber.
The displacing solution pushes the remaining acrylamide solution out of the V-well
into the gel cassettes. As gels contract during polymerization, the displacement
solution is drawn in from the bottom.
I
MPORTANT
Apply overlay immediately! See “Applying Overlay to DALT Slab
Gels” on page 22.
11. Allow non-gradient gels to polymerize for at least 1 hour.