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Allsheng Auto Pure 24, Operation Manual

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Foreword

Thank you for purchasing our Auto Pure series Nucleic Acid Purification
System. This Manual describes the function and operation of the
instrument. In order to use the instrument properly, please read this
manual carefully before using. Keep it for later use when you meet with
difficulties.

Opening Check

Please check the Instrument and Accessories according to the packing list
when you first open the packing case. If anything wrong or missing, please
contact the distributor or the manufacturer.

HANGZHOU ALLSHENG INSTRUMENTS CO., LTD.

Add: Building 1 & 2, Zheheng Science Park, Zhuantang Town, Xihu

District, Hangzhou, Zhejiang 310024, China

Tel.: 0086 0571 89948289

Fax: 0086 0571 87205673

Website: www.allsheng.com

E mail:

[email protected]

File No.: AS180SM

Version No.: V1.3,Jul. 2020

DOMINIQUE DUTSCHER SAS

Summary of Contents for Auto Pure 24

Page 1: ...Operation Manual V1 3 Auto Pure Series Nucleic Acid Purification System Auto Pure 24 48 96 Hangzhou Allsheng Instruments Co Ltd D O M I N I Q U E D U T S C H E R S A S ...

Page 2: ...heck Please check the Instrument and Accessories according to the packing list when you first open the packing case If anything wrong or missing please contact the distributor or the manufacturer HANGZHOU ALLSHENG INSTRUMENTS CO LTD Add Building 1 2 Zheheng Science Park Zhuantang Town Xihu District Hangzhou Zhejiang 310024 China Tel 0086 0571 89948289 Fax 0086 0571 87205673 Website www allsheng co...

Page 3: ...rument which conforms to class of GB 4793 1 standard Please read this manual carefully before operation The device must be used by experienced personnel with appropriate training The operator should not repair the instrument in case any injury or out of warranty If service required please contact Allsheng or your local distributor for repair Before powering on please make sure the voltage of the p...

Page 4: ...t with a cloth or plastic to protect it from dust Disconnect the power cord from the jack at once in the following cases and contact your local distributor or Allsheng Liquid enters into the Instrument Instrument was rained or watered Abnormal operation such as abnormal sound or smell Instrument dropping or outer shell damaged The function has obviously changed Indicates disposal instruction DO NO...

Page 5: ...ation and storage requirements Ambient temperature 10 35 Relative humidity 70 Atmosphere pressure range 500 1060hpa Locate it in a well ventilated room away from corrosive gas D O M I N I Q U E D U T S C H E R S A S ...

Page 6: ...ch Screen 6 Chapter 4 Operations 7 1 Power Connection 7 2 Kits Installation 7 3 Detailed Operations 8 3 1 Start up Interface 8 3 2 Run Program Interface 8 3 3 Manage Program 15 3 4 System Settings 19 3 5 UV Sterilization 25 3 6 Help 26 Chapter 5 Trouble Shooting 27 1 Troubleshootings 27 2 Software Error Alarm List 28 Chapter 6 Accessory 29 Chapter 7 Abbreviations and Tags 30 1 Abbreviations 30 2 T...

Page 7: ...ith special kits Auto pure series can extract samples of animal plant tissue blood and body fluids etc with different kinds of magnetic bead nucleic acid extraction reagents It is mainly used for the extraction and purification of nucleic acid from human body samples 1 Application This instrument is suitable for the extraction and purification of nucleic acids in animal and plant tissues blood and...

Page 8: ...action time 10 60min time Temperature control module Ambient temperature 120 for lysis and elution Heating time Heating time Ambient temperature 120 7 minutes Temp Accuracy 1 Vibrateand mix 10 different speeds for option Operation 7 inch color touch screen mouse can be connected Programs 8 groups of programs can be preset and can store 100 groups of programs Program management Including create edi...

Page 9: ...Auto Pure 24 48 96 Operation Manual Chapter 2 Specifications 3 3 Overall Dimensions Unit mm Fig 1 D O M I N I Q U E D U T S C H E R S A S ...

Page 10: ...c operation keys displays as well as preparations before starting up Please read this chapter carefully before using this instrument 1 Structures 1 1 Front Fig 2 1 2 Back Fig 3 Power Socket Fuse Power Switch USB Port Fan Screw to remove transparent cover Screw to remove panel Display Shortcut keys and indicator Cabin door D O M I N I Q U E D U T S C H E R S A S ...

Page 11: ...ntenance Fig 4 1 4 Transparent Cover The transparent cover is on the right side of the instrument which is for placing or taking out kits The cover can be removed which makes it convenient matching with automatic liquid transfer wrokstation Fig 5 Heating plate Cabin door Magnetic rod Magnetic rod s tip Transparent cover Screw for quick removel D O M I N I Q U E D U T S C H E R S A S ...

Page 12: ... Touch Screen Fig 6 Display screen Touch screen mouse also can be connected for operation TAB Select shortcut program RUN Start the shortcut program and run the instrument STOP Stop the operation Display screen TAB RUN STOP Indicator light D O M I N I Q U E D U T S C H E R S A S ...

Page 13: ...Kits Installation Open the cabin door put kits on the plate position of the rotary table press position button to turn the rotary table and place all the kits in turn Auto pure24 48 96 are suitable for 24 well kit 48 well kit and 96 well kit separately Fig 7 Position button D O M I N I Q U E D U T S C H E R S A S ...

Page 14: ... on the instrument and make sure the door is closed before start start up interface will comes up Fig 8 Then it will enter into Run Prog interface 3 2 Run Program Interface This interface including two modes shortcut mode and list mode as shown in below Fig 9 and Fig 10 D O M I N I Q U E D U T S C H E R S A S ...

Page 15: ...mode interface if one program selected activated in Shortcut column the icon of the program can be displayed on shortcut interface 8pcs of programs can be activated in maximum at the same time SN Name Modify time and Lock are non editable options D O M I N I Q U E D U T S C H E R S A S ...

Page 16: ...k Run to enter into run interface When running the program the instrument will first detect the presence of the kit on the rotary table If no kit is found on the board of the setup program the program will prompt to confirm whether the following steps can be continued as shown in the figure below Fig 11 D O M I N I Q U E D U T S C H E R S A S ...

Page 17: ...netic rod sleeve automatically If rod sleeves are already installed on the current magnetic rod sleeve rack Sleeve loaded continue will pop up If no magnetic rod sleeve is detected after installing the magnetic rod sleeve No sleeve continue will appear Fig 13 D O M I N I Q U E D U T S C H E R S A S ...

Page 18: ... sleeve is successfully installed the instrument automatically performs the following steps please see Fig 15 Fig 15 In the running interface users can stop pause continue or run the program again The plate with dark blue color number 1 is the working D O M I N I Q U E D U T S C H E R S A S ...

Page 19: ...he completion of the operation the No 8 plate position will be automatically pushed to the transparent cover on the right side 3 2 2 View In the list mode or shortcut mode select the required program and click View button to enter the view interface See Fig 16 Users can view each parameter settings of the program Fig 16 Users can click button in the upper right corner to switch to the graphic disp...

Page 20: ...tton to magnetic parameter absorption interface which displays magnetic parameters of selected step as shown in Fig 18 Steps Run run the program starts from currently selected step Option view settings of the program please see Fig 19 Fig 18 D O M I N I Q U E D U T S C H E R S A S ...

Page 21: ...Auto Pure 24 48 96 Operation Manual Chapter 4 Operations 15 Fig 19 3 3 Manage Program Users can manage all programs in Manage Prog interface Fig 20 D O M I N I Q U E D U T S C H E R S A S ...

Page 22: ... the New or Edit button interface of Fig 23 will appear the main difference between New interface and Edit interface is whether the program name exists or not other operations are similar This interface mainly includes five buttons Insert Delete Option Save and Back Insert click Insert to add a new program with default parameters next to the current selected program the new program should be with ...

Page 23: ...interval time between two steps Volume The volume is automatically converted to the amplitude of mixing according to the formula Mix speed 10 kinds of mix speeds from 1 to 10 The higher the value is the faster the mixing speed will be Temp The temperature can be set according to actual requirements only No 2 and 8 wells can be set Click to enter parameter settings of magnetic absorption see below ...

Page 24: ...alling off due to liquid surface tension Anti splash the standing time when magnetic rods pulling away from liquid level after finishing magnetic absorption in case cross contamination which caused by liquid splashing due to some special sample tissues falling off 1 5 Segment time independent magnetic absorption time of each segment the maximum time can reach to 999 seconds Estimated The estimated...

Page 25: ...d then the magnetic rod sleeve frame starts to work Start when It indicates that the magnetic rod sleeve frame starts to work when the temperature rised to the set temperature which is lower than the target temperature Cooling Setup It is used to set the cooling type 3 3 4 Save As Delete In the Manage prog interface click the save as button to save the file and click the delete button to delete th...

Page 26: ...nual Chapter 4 Operations 20 Fig 25 3 4 1 System Time Click Date time button to enter modification interface as shown in the figure below Fig 26 The date and time can be adjusted by or buttons D O M I N I Q U E D U T S C H E R S A S ...

Page 27: ...hapter 4 Operations 21 3 4 2 Language Settings Two options Chinese and English Fig 27 Select the language press Ok to save the modification 3 4 3 Fan Click Air Ejector Fan to choose On or Off Fig 28 D O M I N I Q U E D U T S C H E R S A S ...

Page 28: ...9 Press the Import to enter U disk directory and then select the program needed press the Ok to import Press the export button to enter the system directory select programs and then Ok to export files to the U disk 3 4 5 Software Upgrade Click Upgrade to upgrade interface see Fig 30 please D O M I N I Q U E D U T S C H E R S A S ...

Page 29: ...g 30 Insert the U disk with the latest software in and then upgrade the interface software or control software of the instrument 3 4 6 Operation Record Each run of the program automatically generates a running record Fig 31 D O M I N I Q U E D U T S C H E R S A S ...

Page 30: ...s on while lighting is off if the icon displays Users can click the icon to switch between on and off 3 4 8 Auxiliary function Plate position switch function and Sleeve automatic installation function can be used with the software In the plate position switch interface as Fig 33 below click to choose the plate that you want to switch it to the position of right transparence window as the plate 2 p...

Page 31: ...se the plate position which you want to automatically install uninstall the sleeve as Fig 34 below Fig 34 3 5 UV Sterilization The UV disinfection interface is mainly used for the opening and closing of the UV lamp The time can be set by pressing or button D O M I N I Q U E D U T S C H E R S A S ...

Page 32: ...tically determine half of the set time to sterilize the half circle of the rotary table with a minimum of 2min as shown in the figure below Fig 36 3 6 Help Help interface displays help information and version as shown in the figure below Fig 37 D O M I N I Q U E D U T S C H E R S A S ...

Page 33: ...tube Contact with distributor 4 Can not stop automatically after opening the door Sensor failure Contact with distributor 5 Big variance between actual and display temperature Sensor failure Contact with distributor 6 No heating for heating strip Sensor failure Contact with distributor Heater failure 7 Instrument can t run Controller failure Contact with distributor Motor failure 8 Abnormal sound ...

Page 34: ...od motor sensor damaged E407 Motor position sensor of magnetic rod sleeve damaged E425 Magnetic rod motor position sensor damaged E415 LCD Crystal oscillator Storage code 7 The clock crystal fault E702 Memory chip E2P damaged Setting parameters lost E703 New instrument instrument type hasn t been set Zero has not been calibrated the instrument zero calibration is not in the 3 well will lead to the...

Page 35: ...l Chapter 7 Abbreviations and Tags 29 Chapter 6 Accessory No Name Specs Unit Qty Remark 1 Power cord PCS 1 2 Mouse Logitech PCS 1 3 U disk 8g PCS 1 For upgrading software and transferring programs D O M I N I Q U E D U T S C H E R S A S ...

Page 36: ...ations are for reference and will appear in this operation manual A ampere AC alternating current V volt Hz Hertz W watt USB universal serial bus SD secure digital card WiFi wireless Fidelity Kg kilogram mm millimeter L microliter hpa hectopascal degree centigrade CV stability TAB tab RUN run STOP stop D O M I N I Q U E D U T S C H E R S A S ...

Page 37: ...8 96 Operation Manual Chapter 7 Abbreviations and Tags 31 2 Tags Following marks appear on the instrument Warning label Heating label CONFORMITE EUROPEENNE Be careful of hands D O M I N I Q U E D U T S C H E R S A S ...

Page 38: ...Auto Pure 24 48 96 Operation Manual Notes 32 Notes D O M I N I Q U E D U T S C H E R S A S ...

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