Agilent Technologies G2545A Calibration Manual Download Page 9

Step 4. Calibrate the oven

9

Hybridization Oven Calibration Procedure

Step 4. Calibrate the oven

1

Compare the temperature reading from the 

digital recording 

thermometer

 with the temperature on the digital display on the control 

panel of the 

hybridization oven

.

Make sure that the oven temperature is stable after the 

digital recording 

thermometer

 is removed and before you adjust the 

hybridization oven

The oven temperature is stable when the temperature display on the 
oven reads the current set temperature.

2

If the difference between the two temperature readings is more than 
0.2°C:

a

Press both 

 and 

 at the same time until the two outside decimal 

points of the display begins to flash to put the display into 
calibration mode.

b

While the decimal points are flashing, press the 

 or 

 arrow pad 

until the reading on the display matches that measured by the 

digital 

recording thermometer

.

If the arrow pads are not pressed for five seconds, the display stops 
blinking and reads the temperature in the chamber.

3

To recheck the temperature, start from 

“Step 2. Start the digital 

recording thermometer” 

on page 4. Repeat calibration steps if needed.

The calibration procedure is now complete. Please keep your records as 
required by GLP guidelines.

Summary of Contents for G2545A

Page 1: ...2545A Hybridization Oven Installation Operation and Maintenance Guide p n G2545 9001 for information on site requirements and oven placement Calibrate the Agilent G2545A Hybridization Oven After first installation in a working environment After each 3 months of use When systematic high microarray background noise may indicate a drift in oven temperature Do the calibration steps in this guide to ma...

Page 2: ...cy 0 3 C MicroDAQ com p n LITE5008P 8 000 readings p n LITE5032P 32 000 readings Computer and software to work with the digital recording thermometer Fourtec DataSuite Software or other software as specified by the digital recording thermometer vendor Calibration service to 65 C for digital recording thermometer MicroDAQ com p n CAL NIST TEMP or other service as recommended by digital recording th...

Page 3: ...used during a single hybridization run Install the hybridization chambers without microarrays Distribute the hybridization chambers across hybridization oven rotator rack positions to mimic normal operating procedure 3 Turn on the hybridization oven set rotational speed control to 20 rpm and set oven temperature consistent with the recommended hybridization temperature for the protocol that you us...

Page 4: ...rt the Fourtec DataSuite Software The program opens in Map View 3 Remove the cover on the Fourtec MicroLite II USB digital recording thermometer and insert the digital recording thermometer into a USB port on the computer 4 Check that the Fourtec MicroLite II USB digital recording thermometer is detected by the computer When the Fourtec MicroLite II USB digital recording thermometer is connected t...

Page 5: ...t the recorder 8 Set Interval to 00 05 00 5 minutes 9 Click Setup 10 Click Yes to continue 11 Remove the unit from the USB port and replace the cover 12 Put the digital recording thermometer onto the middle of the hybridization oven rotator rack 13 Secure the digital recording thermometer to the hybridization oven rotator rack Use general purpose laboratory labeling tape or cable ties ...

Page 6: ...er from the oven Let the oven continue to operate after you remove the digital recording thermometer from the oven CAUTION Make sure that the digital recording thermometer is secure and will not detach from the rack If the digital recording thermometer comes loose during use the data collected can be inaccurate A loose digital recording thermometer that becomes trapped and prevents the rotator rac...

Page 7: ... USB port on the computer 3 Right click the Logger icon and click Stop to stop data recording 4 Right click the Logger icon and click Download Data The Fourtec DataSuite Software automatically saves the data on the computer 5 Double click the Logger icon to view a graph of the data 6 To view archived data a Click File Open b In the Open Data Files dialog box select the time period and logger for w...

Page 8: ...al recording thermometer 8 Hybridization Oven Calibration Procedure Figure 1 Temperature reading Period of stable temperature is highlighted Take the temperature reading from the period of stable temperature data recorded before the oven door is opened ...

Page 9: ...he difference between the two temperature readings is more than 0 2 C a Press both and at the same time until the two outside decimal points of the display begins to flash to put the display into calibration mode b While the decimal points are flashing press the or arrow pad until the reading on the display matches that measured by the digital recording thermometer If the arrow pads are not presse...

Page 10: ... Agilent Technologies Inc 2014 Revision A1 February 2014 G2545 90002 G2545 90002 www agilent com ...

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