Agilent Technologies 1260 Infinity, User Manual

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66 HPLC-Chip Cube Interface User’s Guide
5 Operation
Spray Ignition
Spray Ignition
Typically, spray ignition does not require any optimization of the chip
position.
What a spray looks like depends very much on the solvent composition of the
mobile phase. In general, generating a stable spray is more difficult with
aqueous solvents due to the high surface tension of water. The nanospray
process produces both evaporated ions and charged droplets. The system is
designed to separate and collect only evaporated ions while minimizing the
charged droplets which enter the MS inlet as this approach produces the best
S/N.
Table 8 Typical instrument parameters for igniting nanospray
Parameter Value Remarks
• nanopump 0.3 µL/min 98/2 water/ACN (both with 0.1% formic acid)
• loading pump 4 µL/ min 98/2 water/ACN (both with 0.1% formic acid)
• MS: nitrogen 4 L/min
• MS: N2 temperature 300 deg C
• Capillary voltage 1800 V increase if necessary in increments of 50 V
• MS parameters see Chip Cube Interface Adapter guide