Advance acoustic Mupid-2plus, Instruction Manual, Page: 9 / 15

9 8
3 Electrophoresis
Indication line
(1) Place the prepared gel on the gel bed of the migration
tank together with the tray. Since the side near the
power supply is cathode, wells should be placed on
the right for migration of nucleic acids in most cases.
Wipe off any excess gel that is adhering around and
bottom surface of the gel tray.
(2) Pour buffer solution to a level of about 3 mm above
the surface of the gel. However, do not fill above the
level of the buffer indication line. 250-300 mL of
buffer is required.
(3) Connect the power supply to the migration tank and
inset the electric plug into an outlet. The electric
current cannot be turned on with the lid open.
(4) Apply the sample to the wells using a pipette. The
amount of applicable sample depends on the
thickness of the gel; for example, a 4 mm well will
take about 5-8
µ
l and a 6mm well about 8-12
µ
l.
Note that the wells do not correspond to the pitch of
a multipipette.
(1)
(2)
(5) After verifying that the operation switch of the power
supply is OFF, close the migration tank lid.
Do this as follows:
1) First insert the 2 tabs on the cover into the holes in
the migration tank.
2) Then rotate the cover forward to close it.
(6) Set the desired voltage using the output selection
switch.
(7) Push the operation switch to start migration. At this
time verify that the output indicator LED is lit and that
bubbles are forming on the platinum electrodes.
(8) Migration distance may be estimated using the line
printed on the gel tray or the slits on the migration tank
lid. Be sure to turn the operation switch OFF when
sufficient migration has been attained.
(9) Then proceed quickly to the operation of staining the gel.
9P 8P
ON OFF
LED
Migration may be affected if the migration tank
is not placed on a level surface.
When changing the output, make
certain that the operation switch is OFF.
In this case the output indicator LED
will be unlit.
Take care not to put any foreign objects
into or touch inside of the migration
tank during migration.