Advance acoustic Mupid-2plus, Instruction Manual, Page: 8 / 15

10
4 Gel Staining and Band Confirmation
(1)
(1) Verify that the operation switch is OFF, the output
indicator LED is unlit and that the platinum electrode is
not forming bubbles.
(2) Remove the migration tank lid. It can be removed by
grasping the handle on the front of the lid and rotating
it open.
(3) After preparing the dye in a sealable container,
remove the gel tray from the migration tank. Submerge
the gel in the stain solution, either together with the
gel tray or after removing it from the gel tray.
(4) After allowing it to stand for the required amount of time, remove or
de-stain the gel and place it on the transilluminator for observation.
7P 10P
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(6) Before the solution gels, remove air bubbles from
the bottom surface of the gel tray using a glass rod
or other tools. Because bubbles on or in the gel will
affect migration, they should be drawn out with a
dropper, etc.
(7) Let it stand at room temperature for 15-20 minutes to allow
the solution to gel. Gelation may require more time if the
agarose concentration is low.
(8) When the solution has sufficiently gelled, hold the comb at
each end and gently lift it upward. Confirm the rectangular
shape of the wells and the absence of air bubbles.
(9) Grasp the both sides of the gel tray with hands and lift it up and out. Because
the gel is placed in the migration tank together with the gel tray, preparation
is complete at this point.
(2)
If the gel will not be used immediately, pour a small amount of
buffer solution over the gel and wrap it to prevent drying or
contamination.
Because of the danger of
overturning the migration tank,
do not lift the lid directly over it.
Because stains act directly on biological
molecules such as DNA, they are
potentially harmful to the human body.
Handle them carefully, wearing gloves
and avoiding direct contact with the skin.
When using ultraviolet light, be sure to protect eyes and skin
by wearing UV-protective goods such as a faceshield.
Stained gels and buffer solutions, etc. should be disposed of
as directed in the instructions for the stain. Clean off all
stains that adhere to the migration tank, gel tray or other
components.