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EXI-310 INVERTED MICROSCOPE SERIES 

ACCU-SCOPE

®

 

18

TROUBLESHOOTING 

Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If a 
problem occurs, please review the following list and take remedial action as needed. If you cannot solve the 
problem after checking the entire list, please contact your local dealer for assistance. 

OPTICAL

 

PROBLEM 

CAUSE 

SOLUTION 

The illumination is on, but the 
field of view is dark. 

The socket pin is not connected to the 
illumination column 

Connect it securely 

The bulb is burnt out. 

Replace it with a new one 

The brightness is set too low 

Set it to the appropriate position 

Two many filters are stacked 

Reduce them to the minimum required 
number 

The edge of the field of view is 
obscured or not evenly 
illuminated. 

The nosepiece is not in the located position 

Turn the nosepiece into the position where 
you can hear it engaged 

the color filter is not inserted fully 

Push it in all the way 

The phase contrast slider is not located in the 
proper position 

Move the slider until it clicks into place  

Dirt or dust is visible in the field 
of view 

Dirt/dust on the specimen 

Replace with a clean specimen 

Dirt/dust on the eyepiece 

Clean the eyepieces 

The image glares 

The iris diaphragm is closed too much 

Open up the iris diaphragm 

Visibility is poor 

Image is not sharp

Contrast is poor

Details are indistinct

Phase contrast effect
cannot be obtained

The objective is not correctly engaged in the 
light path 

Turn the nosepiece into the engaged position 

the aperture diaphragm is opened or stopped 
down   too far in brightfield observation  

adjust the aperture diaphragm properly 

The lens (condenser, objective, ocular or  
culture dish) become dirty  

Clean it thoroughly 

In the phase contrast observation, the bottom 
thickness of the culture dish is more than 
1.2mm. 

 Use a the culture dish whose bottom 
thickness is less than 1.2mm 

Use a brightfield objective  

Change to the phase contrast objective 

The light annulus of the condenser does not 
match the phase annulus of the objective. 

Adjust the light annulus so that it matches the 
phase annulus of the objectives 

The light annulus and the phase annulus are 
not centered 

Adjust the centering screws to center it 

The objective used is not   compatible with 
phase contrast observation 

Please use the compatible objective 

When looking at the edge of the culture dish, 
the phase contrast ring and the light ring is 
deviated from each other 

Moving the culture dish until you obtain the 
phase contrast effect. You also could 
demount the slider, and set the field 
diaphragm lever to “

” 

One side of the image is 
blurred 

The objective is not in the center of the light 
path 

Insure the nosepiece is in the “clicked” 
position 

The specimen is not correctly mounted on the 
stage. 

 Place the specimen on the stage correctly. 

The optical performance of the culture vessel 
bottom plate is poor (profile irregularity, etc.) 

Use a vessel with a good profile irregularity 
characteristic. 

Summary of Contents for EXI-310-PH

Page 1: ...MANUAL EXI 310 INVERTED MICROSCOPE SERIES 100 Lauman Lane Suite A Hicksville NY 11801 Tel 877 877 7274 Fax 516 801 2046 Email Info nyscopes com www microscopeinternational com...

Page 2: ......

Page 3: ...6 ASSEMBLY DIAGRAM 7 DETAILED ASSEMBLY 8 9 OPERATION ILLUMINATION 10 INTERPUPILLARY DISTANCE 10 FOCUSING 10 ADJUSTABLE TENSION CONTROL 11 STAGE PLATES OPTIONAL 11 SELECTING THE LIGHT PATH 12 APERTURE...

Page 4: ...jectives or focusing assembly 2 Keep the instrument clean remove dirt and debris regularly Accumulated dirt on metal surfaces should be cleaned with a damp cloth More persistent dirt should be removed...

Page 5: ...eded Avoid placing the microscope in dusty surroundings or in high temperature or humid areas as mold and mildew will form Carefully remove the microscope from the EPE foam container by its arm and ba...

Page 6: ...ocus Adjustment Knob Base Eyepieces Phase Contrast Slider Condenser Stage Camera Photo Port Optional Fluorescence Light Path Access Panel NOTE If you did not purchase your EXI 310 Microscope with fluo...

Page 7: ...EXI 310 INVERTED MICROSCOPE SERIES ACCU SCOPE 6 MICROSCOPE DIMENSIONS...

Page 8: ...ly Use the 1 5mm and 2mm hex wrenches that are supplied with your microscope when required Be sure to keep these wrenches for changing out components or making adjustments When assembling the microsco...

Page 9: ...n 2 Remove the nosepiece cap closest to you and thread the lowest magnification objective onto the nosepiece opening then rotate the nosepiece clockwise and thread the other objectives from low to hig...

Page 10: ...put voltage indicated on the rear label of the microscope corresponds to your line voltage The use of a different input voltage than indicated will cause severe damage to your microscope Connecting th...

Page 11: ...ete circle should be seen in the viewing field when viewing the specimen slide An improper adjustment will cause operator fatigue and will disrupt the objective parfocality Where the on the eyepiece t...

Page 12: ...tment ring clockwise to loosen or counterclockwise to tighten according to user preference Fig 8 Using the Stage Plates Optional Fig 9 10 NOTE for optimal viewing ensure the thickness marked on each o...

Page 13: ...right Slide the light path selection slider to the left to the IN position This will send 20 of the light to the binocular viewing head and 80 to the top photo port for HDMI digital imaging and docume...

Page 14: ...the iris diaphragm lever to 75 of the N A of the objective in use Phase Contrast Observation Depending on the configuration ordered the EXI 310 can be used for phase contrast observation with LWD pha...

Page 15: ...er is pre aligned at our facilities If realignment is necessary follow these steps 1 Place a specimen on the stage and bring it into focus 2 Replace the eyepiece in the eyepiece tube with the centerin...

Page 16: ...sistences This can reduce image contrast If this occurs repeat steps 1 5 for readjustment The centering procedure may have to be repeated in order to get the best possible contrast if a specimen slide...

Page 17: ...lider and push rod for filter position and selection Each position of the slider clicks securely into position due to click stop ball bearing positioning Alternate light sources are available for meta...

Page 18: ...specimen through the eyepieces and open the field iris diaphragm gradually to find the image in the field of view If your image is not centered in the field of view adjust the two screws at both sides...

Page 19: ...ase contrast effect cannot be obtained The objective is not correctly engaged in the light path Turn the nosepiece into the engaged position the aperture diaphragm is opened or stopped down too far in...

Page 20: ...289 2228 for an authorized service center near you The light intensity is not enough Not use an designated lamp use an designated lamp The brightness adjustment knob is used wrong Adjust the brightnes...

Page 21: ...haser LED lamps are warranted for a period of two years from the date of original invoice to the original end user purchaser The mercury power supply is warranted for a period of one year from the dat...

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