CELL-DYN
®
3200 System Operator’s Manual
10-27
9140181H—October 2001
Section 10
Troubleshooting and Diagnostics
Troubleshooting the “Sampling error-incomplete aspiration” Message
1. Check to see if the problem occurs in both the Open and Closed Modes of
operation. If the problem is confined to one mode only, the other may be
eliminated as the cause of the problem.
2. Determine whether the problem is a true incomplete aspiration. Run a sample
and verify that blood is visible in the sample tubing above the appropriate
probe or needle.
3. Verify that blood is pulled through the Shear Valve. Blood should be visible in
the lines (approximately one inch) on both sides of the Shear Valve before it
rotates.
Troubleshooting a Flow Error Message
1. The
flow error
messages indicate a problem with the kinetic rate of the WBC,
RBC/PLT, or NOC measurements. The kinetic information is only available
immediately after the cycle is finished. Therefore, the screen should be
printed immediately after the flow error occurs.
2. From the first
DIAGNOSTICS MENU
screen, press
[CNT RATE SUMMARY]
.
3. Press the appropriate
[COUNT RATE]
key (WOC, RBC, PLT, or NOC) to
display the data for the kinetic rate. Press
[PRINT]
to obtain a printout.
4. Press the appropriate
[COUNT GRAPH]
key to display the graph of the kinetic
data. Press
[PRINT]
to obtain a printout.
5. Configure the
RUN
screen to display the Size/Complexity scatterplot and the
NLM histogram. (If necessary, refer to the instructions given in
; Subsection:
Customize Displayed Report
.) Obtain
several printouts. This information can help to determine if the flow is erratic or
just momentarily interrupted.
Troubleshooting Imprecise or Inaccurate Data
1. Obtain a normal blood sample. Select an empty QC file and run a minimum
of ten Open Mode runs into the file. Obtain a printout.
2. Run a minimum of nine Closed Mode runs into the same file. (For the
CELL-DYN 3200SL, aliquot a sample into three tubes and run each tube
three times.) Use the
[REJECT SPECIMEN]
key to reject the Open Mode runs
and obtain a printout. This information can be used to determine if the
problem is mode or measurement related.
3. Obtain printouts of related data as indicated in the following steps.