3-18
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Principles of Operation
Flow Cytometry
Section 3
WBC Parameters
Figure 3.8 WBC Data and Scatterplots
The WBC data is generally displayed as depicted in Figure 3.8. All numeric and
graphic data are automatically displayed on the
RUN
screen in the format selected.
After the WBC scatter information has been plotted and the cells have been
classified into the five subpopulations, the algorithms then determine the WBC and
the percent of cells in each subpopulation.
Once the WBC count is determined, the absolute number of cells in each
subpopulation is calculated by multiplying that WBC count by the percentage. The
results are expressed as follows:
WBC
# x K/µL
NEU
# x K/µL and %
LYM
# x K/µL and %
MONO
# x K/µL and %
EOS
# x K/µL and %
BASO
# x K/µL and %
The decimal point moves to display up to three decimal places for the absolute
number and percent.