PANNORAMIC® DESK DW II 3.0.4
SCANNING MODES AND SETTINGS
MARCH 30, 2021
57
Multilayer mode
•
Select
Single layer
option if you want to scan a single layer of the slide.
•
Extended focus:
By choosing the
Extended focus
option, the software selects the
sharpest image from each focus level for each image field and combines them into one
single image. Therefore, those parts that were blurry in auto/manual focus will also be
sharp. This method guarantees the maximum depth of sharpness; however, it reduces
digitization speed.
Figure 40 – Focus level and step size
•
Z-Stack:
For
Extended focus
and
Z-Stack
scanning methods, you can determine the
desired number of focus levels with the
Levels
selection box. The current focus value is
the initial level. If you set
5
for example, then the initial level, plus four (two focus levels
above and below the FOVs) are captured. Therefore, it is advised to choose an odd
number for the focus levels. Separate layers are created and stored this way and can be
opened with Viewer applications. The Z-Stack slide contains the extended focus layers.
Figure 41 Focus level settings
Step size
determines the spacing between focus levels. One step is around 0.2 µm. Default
settings are:
Levels
: 7;
Step size
: 5.
Scanning Settings
Includes the
Stop and Go
scanning mode.
Use Color matrix:
Select an applicable preset color profile to be used for Brightfield
scanning from the drop-down menu. Settings according to the profile has effect on the
color set, color temperature, and gamma value of the scanned image.
