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3D Cultures 3D Bio-Printer, User Manual

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3D Cultures 3D Bio-Printer User Manual Download Page 2

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TABLE OF CONTENTS 

SAFETY WARNINGS & PRECAUTIONS

 ................................................................................................. 2 

OVERVIEW

 ................................................................................................................................................. 3 

PACKAGE CONTENTS

 ............................................................................................................................. 3 

FEATURES

 ................................................................................................................................................... 4 

CUSTOMER SERVICE & TECHNICAL SUPPORT

 .................................................................................. 4 

USING THE INTERACTIVE SCREEN SYSTEM

 ...................................................................................... 4 

SOFTWARE INSTALLATION

.................................................................................................................... 4 

Installing Cura for Windows

....................................................................................................................... 4 

Installing Repetier-Host

 ........................................................................................................................... 10 

PRINTER & NOZZLE SETUP

 ................................................................................................................. 11 

Out of Box Setup

 ..................................................................................................................................... 11 

Zeroing the nozzle

 ....................................................................................................................................... 12 

REGULATORY COMPLIANCE

 ............................................................................................................... 15 

 

 

 

Summary of Contents for 3D Bio-Printer

Page 1: ...ry Preheating is not recommend with the use of cells This manual and specifications described herein are subject to change without notice All trademarks are the sole property of their respective companies This product does not provide a User Interface but reference many that are helpful Copyright 2017 3D Cultures All Rights Reserves ...

Page 2: ...KAGE CONTENTS 3 FEATURES 4 CUSTOMER SERVICE TECHNICAL SUPPORT 4 USING THE INTERACTIVE SCREEN SYSTEM 4 SOFTWARE INSTALLATION 4 Installing Cura for Windows 4 Installing Repetier Host 10 PRINTER NOZZLE SETUP 11 Out of Box Setup 11 Zeroing the nozzle 12 REGULATORY COMPLIANCE 15 ...

Page 3: ...ld fall and cause wither personal injury or damage to the device and or other equipment Do not submerge the device in any liquid Do not subject the product to extreme force shock or fluctuation in temperature or humidity This device is intended for indoor use only Do not expose this device to water or moisture of any kind Do not place drinks or other containers with moisture on or near the device ...

Page 4: ...ingle extruder bio head It uses a standard BD 10mL syringe with interchangeable luer lock tips This printer can print a variety of materials including but is not limited to gelatin alginate chitosan collagen etc The limitation of printing is the material viscosity Thick materials combined with extremely small tips may prove to be more difficult to print compared to thinner materials and larger tip...

Page 5: ...otating the control wheel clockwise while editing a value increases that value 6 Pressing the center of the control wheel will activate the selected menu item or to save an edited value SOFTWARE INSTALLATION The recommended method of printing is directly from a gcode file on the microSD card This method does not require a continuous connection to your computer Gcode files can be downloaded to the ...

Page 6: ...es are checked then click install to continue 4 Once the files have been extracted and Cura installation is complete the driver installation wizard will launch Click next to continue 5 Click Finish to complete the driver installation ...

Page 7: ...on is checked then click Finish to complete the installation and launch the program 8 Wait for Cura to finish launching If this is the first time you have run Cura on this machine Cura will automatically launch the First time run wizard Otherwise you will need to select Machine Add new machine ...

Page 8: ...7 P a g e 9 Click the button next to other option then click Next button to continue 10 Select the Custom Option then click Next to continue ...

Page 9: ...ince these interface are not set for bio materials You will have to manually set the temperature and extrusion rate Note The fill density support type platform adhesion types temperature etc options should be set according to the needs of the model you are printing The fill density will determine the overall strength of the finished object and the internal archetiture It is not recommended to use ...

Page 10: ...lly using the included Micro USB cable plug one end into the usb port on the printer then plug the other end into an available USB port on the your computer Watch the right hand corner of the screen A bubble will appear in the lower right corner of your computer which is telling you that windows is installing the necessary printer drivers Click the bubble and verify the the drivers have been prope...

Page 11: ...lling Repetier Host You can also install Repetier Host from the file on the included microSD card from an internet download The screenshots below shows the proper settings to use for this bio printer in Repertier Host ...

Page 12: ...tilation and a nearby AC power outlet 2 Remove the any tape or cable ties holding the nozzle a If the nozzle is not connected to the motion arm There are a series on mounting holes for the nozzle figure below left line up to your desired placement and use the hex tool to tighten both screws figure below right b Future adjustments can be made by moving to different holes or moving the nozzle up and...

Page 13: ... the nozzle Zeroing the nozzle ensures that the tip is always set to a reference zero position Since most tips have a different length you would want to ensure when a tip is changed the reference zero point is calibrated To set the reference zero do the following 1 Do NOT install the syringe as yet 2 Insert the microSD card and run the code NOZZLESETUP gcode This code will move the nozzle to the p...

Page 14: ...13 P a g e 5 Insert the syringe with the tip 6 Position the nozzle with the syringe where the tip barely touches the base of the petri dish 7 Tighten both hex screw with the hex tool ...

Page 15: ...your nozzle is now at the zero Z position and is ready to print 10 Note Ensure the nozzle is at zero position for best printing If printing into a petri dish ensure your code takes into consideration the dimensions parameters of the dish a DO NO Pre heat if working with cells ...

Page 16: ...tions are not permitted Any modifications are at your own risk This device is not intended to be used on humans or animals This device may use human or any products for research and development purposes only Any biological materials used must be from an approved source Use at your own risk Microsoft and windows are registered trademarks of Microsoft Corporation in the United States and or other co...

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