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Model 408 NO Calibration Source Manual Rev. D-3 

12 

4.6.2  Fm2 (output flow cal factor) 

This is the cal factor for the output air flow of the 408 NO Calibration Source

.  If the 

user  has access  to a reliable  flow  meter  and finds  a difference  in  actual flow  versus 
the flow readout, this can be adjusted as needed. To determine the flow cal factor, set 
Fm2 =1.00 then use: (Actual vol. flow/readout vol. flow)=Fm2 cal factor and adjust this 
cal factor as needed. 

4.6.3  NO (NO cal factors): 

Selecting this option takes the user to the 408 NO cal factors and the LCD will display, 
 

NO Cal Menu 

 

Cal_l     Cal_h   

 

 

The 408 NO Calibration Source

 makes use of 2 sets of calibration factors both with 

Z  (offset)  and  S  (slope  or  sensitivity)  for  different  selected  NO  concentrations.  Cal_l 
menu  contains  the  low  concentrations  cal  factors  and  uses  these  factors  when 
selecting  concentrations  of  20-250  ppb.  Cal_h  is  a  different  set  of  cal  factors  used 
when  selecting  concentrations  251-1000  ppb.  Every  408  NO  Calibration  Source

  is 

shipped with a NIST-traceable calibration.  Both Cal_l and Cal_h calibration factors (

Z

 

and 

S

are set in the instrument and recorded in the instrument’s calibration document 

and  birth  certificate.    However,  you  may  choose  to  change  the  calibration  factors  so 
that the instrument output agrees with your own nitric oxide standard.  You can always 
return  the  instrument  to  its  factory  calibration  if  desired.    To  change  the  calibration 
factor,  place  the  cursor  over  the  digit  (

x

)  you  wish  to  change  and  hold  in  the  Select 

Switch until the cursor blinks.  You may now change the value of the digit by rotating 
the  Select  Switch.    Once  the  desired  value  of  a  digit  is  selected,  momentarily  press 
the  Select  Switch  such  that  the  cursor  no  longer  blinks.    You  may  now  rotate  the 
Select  Switch  to  choose  another  digit  to  change.    The 

Z

 

parameter  is  a  “zero”  or 

“offset”, and the 

S

 parame

ter is a “gain” (“slope” or “sensitivity”) factor.  Increasing the 

Z

  parameter  increases  the  concentration  of  NO  produced  in  an  additive  way.    For 

example, changing 

Z

 from 1.3 to 2.4 will cause the NO output to increase by 1.1 ppb.  

Increasing  the 

S

  parameter  increases  the  NO  output  concentration  linearly;  i.e.,  for 

S=1.10,  the  NO  concentration produced  will  be  10%  higher  than for  S=1.00,  and for 
S=0.95 it will be 5% lower.  Once the desired value of a digit is selected, momentarily 
press the Select Switch such that the cursor no longer blinks.  You may now exit the 

Cal

 submenu by selecting and clicking on 

.

 

4.7 

Error Codes 

Error codes are displayed if any of the four key parameters (temperature, air flow rate, 
N

2

O flow rate, and lamp intensity) are out of range as follows: 

 

Code 

Error 

N

2

O flow out of range (40 

– 80 mL/min) 

Air flow rate out of range (3.3 

– 3.8 L/min) 

Summary of Contents for Model 408

Page 1: ...nual Rev D 3 i Nitric Oxide Calibration Source 2B Technologies OPERATION MANUAL Model 408 Copyright 2018 2B Technologies All rights reserved Technical Support https twobtech com tech support html techsupport twobtech com 1 303 273 0559 ...

Page 2: ...ttaching the NO Calibration Source to a NO Monitor 6 4 OPERATION 7 4 1 Flw Flow Menu 8 4 2 Adv Advanced Menu 8 4 2 1 Cfg Configuration Menu 8 4 2 2 NO Nitric Oxide Menu 10 4 2 3 Zer Zero Menu 10 4 3 Reentering the Menu 10 4 4 Viewing of Flow Rates Outside of the Menu 10 4 5 Collecting Diagnostic Data over the Serial Port in Real Time 11 4 6 Cal Menu 11 4 6 1 Fm1 N2O flow cal factor 11 4 6 2 Fm2 ou...

Page 3: ...Model 408 NO Calibration Source Manual Rev D 3 iii IDENTIFICATION RECORDS Record the following information for future reference Unit serial number Warranty start date date of receipt ...

Page 4: ... February 2008 Revision D March 2015 Revision D 2 December 2017 Several corrections edits for clarification and formatting changes were made Revision D 3 November 2018 Updated schematic in Section 1 2 updated web links other minor copy edits TRADEMARKS PATENTS 2B Technologies 2B Tech 2B and NO Calibration Source are trademarks of 2B Technologies CONFIDENTIALITY The information contained in this ma...

Page 5: ...logies for repair or replacement Prior to returning the product a Returned Merchandise Authorization RMA number must be obtained from the 2B Technologies Service Department and an RMA form completed The RMA form allows us to identify the owner of the instrument and provides a return shipping address Please do not return any of the accessories power pack serial cable connecting tubing etc with the ...

Page 6: ...n and no responsibility is taken for any errors that may be present In no event shall 2B Technologies be liable for incidental or consequential damages in connection with or arising from the use of the Nitric Oxide Calibration Source manual and its accompanying related materials Warranty is valid only for the country designated on the 2B Technologies quote or invoice ...

Page 7: ...so used as a general anesthetic at concentrations up to 50 and is thus well tolerated by humans There are no known toxicological effects of N2O other than asphyxiation at extremely highly concentrations due to exclusion of oxygen N2O is not itself combustible but it strongly supports combustion of organic materials and reducing agents Contact with liquid N2O can cause frost bite due to cooling as ...

Page 8: ...HINWEIS Vor dem Öffnen des Gerätes Netzstecker ziehen WARNHINWEIS Dieses auf dem Gerät weist darauf hin dab der Anwender zuerst das entsprechende Kapitel in der Bedienungsanleitung lesen sollte WARNHINWEIS Wenn das Gerät nicht wie durch die Firma 2B Technologies USA vorgeschrieben und im Handbuch beschrieben betrieben wird können die im Gerät eingebauten Schutzvorrichtungen beeinträchtigt werden I...

Page 9: ...ntration of NO in ambient air containing the same level of humidity as the air sample to be measured The NO Calibration Source is factory calibrated against a NIST traceable standard However the calibration parameters may be changed in the menu in case the user wants to recalibrate the NO Calibration Source against a separately maintained standard i e the NO Calibration Source can be used as a tra...

Page 10: ... of the NO Calibration Source An air pump draws air in through NO and ozone scrubbers to remove any ambient NO The air flow rate is controlled by use of restrictors not shown and a needle valve that vents part of the flow and is measured by a flow meter A constant fraction of the flow bypasses the flow meter not shown so that the flow through the meter is in the range 0 1 L min The needle valve is...

Page 11: ... at wavelengths near 185 nm Measurements of the output of the photolysis chamber show that no insignificant concentration of NO2 is produced The lamp intensity at 254 nm is monitored by a photodiode and controlled by the microprocessor The photodiode voltage is calibrated against the output NO concentration Pressure within the gas stream is measured but not controlled Instead the lamp intensity is...

Page 12: ...o 60 C Relative Humidity Range 0 80 non condensing Altitude Range 0 2000 meters Power Requirements 12 V dc or 120 240 V ac 50 60 Hz 2 5 A 21 watt Size 3 7 8 5 9 5 in 9 4 21 6 24 1 cm Weight 5 2 lb 2 4 kg Ratings for Usage Indoor use only ordinary protection not protected against harmful ingress of moisture Class I Equipment grounding required for safety Installation Overvoltage Category II for Tra...

Page 13: ...out using a single cartridge The pressure inside the cartridge remains nearly constant as N2O evaporates and flows out of the cartridge The evaporation of N2O causes the N2O cartridge to cool which in turn slightly reduces the internal pressure of 50 atmospheres The Model 408 comes with an attached Portable N2O Source see photo below To use an N2O cartridge follow these steps 1 Make sure N2O flow ...

Page 14: ...E Teflon PFA or some other inert material that does not destroy NO and that does not desorb plasticizers and other organics that can contaminate the flow path The length of tubing should be kept as short as possible not more than a few feet to minimize NO loss and an unrestrictive over flow T is needed at the inlet of the NO monitor to be calibrated Tygon polypropylene which may look like Teflon a...

Page 15: ...ollowed by a prompt to open the regulator of the N2O cartridge and set the flow rate Target 60 10 N2O Flow xx x where xx x is the measured N2O flow rate At this point turn the black knob on the N2O regulator until the measured flow is 60 mL min Once the flow is in range press the black Select Switch on the front panel of the instrument When the menu appears release the Select Switch Note You may e...

Page 16: ...enu for displaying error code definitions across the LCD The user can scroll through the error code definitions by rotating the Select Switch The list of error codes and definitions can be found in Section 4 7 of this manual below Prg is a submenu for defining a programmed series of step NO concentrations Choosing Prg results in the following submenu Prg Menu Num Steps x Note In order to obtain ac...

Page 17: ...et NO concentration in ppb that is currently being used for the first step of the program You may now change the NO concentration for the first concentration step in the same way that the Time step was changed Executing will bring up the following submenu and allow you to set the target concentration for the next step Select NO Step Step 2 xxxx This process is repeated until the target concentrati...

Page 18: ...ncentration five bars The selected target NO concentration will remain in memory until the next time you change it Bear in mind that the instrument will output 20 1 000 ppb of NO reliably It is not recommended using the 408 for concentrations less than 20ppb other than zero or concentrations greater than 1000 ppb 4 2 3 Zer Zero Menu Selecting Zer from the Adv menu allows the user to produce zero a...

Page 19: ...d sheet program where it may be converted to formatted data in columns by defining delimiters such as commas and carriage returns for data manipulation and graphing For data transfer the correct settings are Baud rate 19 200 Data bits 8 Parity None Stop bits 1 Flow control None or hardware Serial data lines are output approximately once every second as follows Lamp Intensity arbitrary units Temper...

Page 20: ...that the instrument output agrees with your own nitric oxide standard You can always return the instrument to its factory calibration if desired To change the calibration factor place the cursor over the digit x you wish to change and hold in the Select Switch until the cursor blinks You may now change the value of the digit by rotating the Select Switch Once the desired value of a digit is select...

Page 21: ... D 3 13 3 1 and 2 4 Temperature out of range 312 316 K 5 1 and 4 6 2 and 4 7 1 2 and 4 8 Ratio of lamp intensity to target intensity out of range 0 96 to 1 04 9 1 and 8 10 2 and 8 11 1 2 and 8 12 4 and 8 13 1 4 and 8 14 2 4 and 8 15 1 2 4 and 8 ...

Page 22: ...ariation in response making it necessary to periodically check the calibration We recommend calibration at least annually The instrument may be returned to 2B Technologies for calibration servicing Troubleshooting If the instrument fails to operate correctly common problems can be identified and corrected using Table 5 1 next page Technical Support for Calibration and Troubleshooting For calibrati...

Page 23: ...lace air pump Display is blank or nonsense Bad connection of display to circuit board Remove top cover and reconnect display to circuit board Check solder connections to display Target temperature is never reached Absent or loose connection of temperature probe cable to circuit board Heater is burned out Remove top cover and reattach connector to circuit board Replace cartridge heater Instrument d...

Page 24: ...lection devices require a cross over cable in which pins 1 and 3 are exchanged between the two ends of the cable Use a cross over cable or additional connector that switches pins 1 and 3 Make sure that the baud rate on your data collection program e g Tera Term or Hyperterminal is set to 19200 Make sure that the com port assigned by your computer matches the com port setting in your data collectio...

Page 25: ...tion 3 of this manual for a labeled front view of the instrument Figure 6 1 Top view of NO Calibration Source with top cover removed Pump Buffer Volume N2O Flow Meter Power Switch UV Lamp Air Flow Meter Pressure Relief Valve Air Pump O3 Scrubber NO Scrubbers Photolyis Chamber VSO Valve ...

Page 26: ...ion Source Manual Rev D 3 18 Figure 6 2 Rear view of NO Calibration Source Serial Port Needle Valve to Adjust Air Flow Regulator for N2O Flow Adjustment N2O Cartridge Holder Power Switch NO Air Out N2O In Power 12 V 2 5 Amp ...

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