10x Genomics Chromium X Series, User Manual

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CG000396 | Rev C
User Guide | Chromium X Series (X/iX) | Tips & Best Practices
Link to TOC | 10xgenomics.com
Chip & Holder Assembly
• Close the holder lid. Attach the gasket by holding the tongue (curved
end, to the right) and hook the gasket on the left-hand tabs of the
holder. Gently pull the gasket toward the right and hook it on the two
right-hand tabs.
• DO NOT touch the smooth side of the gasket.
• Open the chip holder.
• Align notch on the chip (upper left corner) and the open holder with
the gasket attached.
• Slide the chip to the left until the guide on the holder is inserted into
the chip. Depress the right hand side of the chip until the spring-
loaded clip engages.
• Keep the assembled unit with the attached gasket until ready for
dispensing reagents into the wells.
Chip Loading
• Place the assembled chip and holder flat (gasket attached) on the bench
with the lid open.
• Dispense at the bottom of the wells without introducing bubbles.
• When dispensing Gel Beads into the chip, wait for the remainder to
drain into the bottom of the pipette tips and dispense again to ensure
complete transfer.
• Refer to the “Load Chromium Next GEM Chip” section of the
appropriate assay kit user guide for specific instructions.
Chip in Chromium X Secondary Holder
Clip
Chromium X
Secondary Holder
with Gasket
Assembled
Chip
Chromium
Next GEM Chip
contd.