10x Genomics Chromium X Series User Manual Download Page 1

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10xgenomics.com

User Guide | CG000396 | Rev C

Chromium X Series (X/iX)

with Readiness Test

For use with:

Chromium X

& Accessory Kit, 12-Month Warranty, PN-1000331

Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327

Chromium X

& Accessory Kit, 24-Month Warranty, PN-1000332

Chromium iX Accessory Kit, PN-1000323 | Chromium X Upgrade Kit, PN-1000327

Chromium iX

& Accessory Kit, 12-Month Warranty, PN-1000328

Chromium iX Accessory Kit, PN-1000323

Chromium iX

Accessory Kit, 24-Month Warranty, PN-1000329

Chromium iX Accessory Kit, PN-1000323

Summary of Contents for Chromium X Series

Page 1: ...ccessory Kit PN 1000323 Chromium X Upgrade Kit PN 1000327 Chromium X Accessory Kit 24 Month Warranty PN 1000332 Chromium iX Accessory Kit PN 1000323 Chromium X Upgrade Kit PN 1000327 Chromium iX Accessory Kit 12 Month Warranty PN 1000328 Chromium iX Accessory Kit PN 1000323 Chromium iX Accessory Kit 24 Month Warranty PN 1000329 Chromium iX Accessory Kit PN 1000323 ...

Page 2: ...does not claim any rights in those third party marks or names 10x Genomics products may be covered by one or more of the patents as indicated at www 10xgenomics com patents The use of products described herein is subject to 10x Genomics Terms and Conditions of Sale available at www 10xgenomics com legal notices or such other terms that have been agreed to in writing between 10x Genomics and user A...

Page 3: ... 10xgenomics com Document Revision Summary Document Number CG000396 Rev C Title Chromium X Series X iX User Guide with Readiness Test Revision Rev B to Rev C Revision Date September 2021 Specific Changes Network connectivity recommendations updated on pages 17 26 and 27 General Changes N A ...

Page 4: ...Components 11 System Components 12 Accessory Kits 13 System Operation 14 Step 1 Installation 15 Step 2 First Time Use 17 Step 3 Readiness Test 18 Step 4 Main One Touch Operation 19 Tips Best Practices 21 Icons 22 Chromium Next GEM Chip 22 Chip Placement on Chromium X Tray 24 10x Genomics Cloud 24 Menu Options 25 Menu Options 26 Firmware Update Procedure 28 Maintenance 29 System Maintenance 30 Movi...

Page 5: ...Introduction 6 Introduction 7 Product Identification 7 Product Specifications ...

Page 6: ...hannel High throughput 2 000 20 000 cells per channel 2 000 60 000 cells per channel with CellPlex Standard assay 500 10 000 cells per channel 500 30 000 cells per channel with CellPlex The Chromium X Series is one instrument available in two firmware options Chromium X and Chromium iX Both the standard Next GEM chip and holder silver color and the HT Next GEM chip and holder black color are compa...

Page 7: ...Hz 250 W 90 264 V operational range 10 of nominal Overvoltage Category II standard receptacle Pollution Degree 2 Indoor Use Only Ventilation Requirement Minimum 4 10 cm Around all sides Operating Temperature 64 82 F 18 28 C Use in a typical indoor laboratory environment Extreme temperature conditions will affect the sensitive reagents used with the instrument Humidity 30 80 R H non condensing Alti...

Page 8: ...Safety Compliance Information 9 Safety 10 Regulatory ...

Page 9: ...e Hazardous Materials symbol indicates the presence of materials that are toxic or otherwise harmful to the operator if handled incorrectly The Biohazard symbol indicates the presence of biological samples that can be harmful to the operator if handled incorrectly Ensure ground is reliably connected before plugging the instrument s power cord into the power source receptacle Grounding is required ...

Page 10: ...s for harmonic current emissions equipment input current 16 A per phase EN 61000 3 3 Electromagnetic compatibility EMC Part 3 3 Limits Limitation of voltage changes voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current 16 A per phase and not subject to conditional connection RoHS Directive 2011 65 EU Restriction of the use of certain hazardous subst...

Page 11: ...System Components 12 System Components 13 Chromium X iX Accessory Kits ...

Page 12: ...onents Link to TOC 10xgenomics com Back Power Supply USB Ports Ethernet Port Pressure Release Valve See Troubleshooting Fan Tray Locater Pins Rim for chip holder placement Indentations for chip holder feet Finger Access Area System Components Front Touchscreen Tray ...

Page 13: ... 13 Detachable Adapters 1 3000603 USB 8 GB 1 3000664 Product Part Number Item Chromium X Chip Holder 2 3000598 Chip Gasket HT 5 Pack 1 3000614 10x Magnetic Separator HT 1 2000431 USB 8 GB 1 3000664 The Chromium X Series instrument is compatible with VFAT and exFAT formatted USBs Product Part Number Item Shipping Readiness Test Block 1 3000652 Chip Gasket HT 1 3000613 The shipping readiness test bl...

Page 14: ...System Operation 15 Installation 17 First Time Use 18 Readiness Test 19 Main One Touch Operation ...

Page 15: ... the instrument Ensure ground is reliably connected before plugging the instrument s power cord into the power source receptacle Grounding is required to prevent electric shock If the power source is not grounded qualified personnel must first install a reliable safety ground Includes a level sturdy laboratory bench that is not subject to movement or vibration Provides a minimum of 4 10 cm clearan...

Page 16: ...um X iX instrument out of the box and place on bench top Remove all transportation packaging materials and tape e Within the Accessory Kit box locate the universal power cord and plug adaptors Attach the correct country adaptor to the universal adaptor f Plug the Chromium X iX into a properly grounded outlet of proper voltage DO NOT power on yet g If using an Ethernet cable not provided to connect...

Page 17: ...e unit b The touchscreen will display an initialization progress bar followed by a welcome screen c Walk through the Internet Connectivity Setup optional and the Date and Time Setup d On a networked device login to your 10x Genomics account at activate 10xgenomics com and register the instrument Enter the serial number instrument ID Read and accept the legal agreement e Update the firmware Network...

Page 18: ...ay c After the test is complete follow screen instruction to remove the block with gasket from the tray d Store the assembled block and gasket in a plastic bag away from sun light and extreme temperature Retain for future use e A successful readiness test marks the end of setup The instrument is now ready for use Initiate subsequent readiness tests through the home screen by loading the shipping r...

Page 19: ...min it will automatically close on its own System requires a few seconds before the tray can be reopened again Pinch risk Ensure no obstructions or fingers present near closing tray The home screen will now display a play symbol and the name of the chip type Use Eject button in lower left corner of the screen to abort i Press the play button to start the run Midrun progress bar will show the time ...

Page 20: ...X System Operation Link to TOC 10xgenomics com j At the end of a run the button will display an eject symbol and a Run Info tab at the bottom of the screen Green indicates a successful completed run Red indicates a failed run error see Troubleshooting ...

Page 21: ...Tips Best Practices 22 Chromium Next GEM Chip 24 Chip Placement on Chromium X Tray 24 10x Genomics Cloud ...

Page 22: ...of the appropriate assay kit user guide for specific instructions Fill all unused paired input wells on a chip with an appropriate volume of 50 glycerol solution before loading the used wells Avoid contacting the bottom surface of the chip with gloved hands and other surfaces Frictional charging can lead to inadequate priming of the channels potentially leading to either clogs or wetting failures ...

Page 23: ...erted into the chip Depress the right hand side of the chip until the spring loaded clip engages Keep the assembled unit with the attached gasket until ready for dispensing reagents into the wells Chip Loading Place the assembled chip and holder flat gasket attached on the bench with the lid open Dispense at the bottom of the wells without introducing bubbles When dispensing Gel Beads into the chi...

Page 24: ...oning Oil Indentations and locater pins assist in proper placement of the assembled chip and holder on the instrument tray The 10x Genomics Cloud at cloud 10xgenomics com is a universal user account for all of the web services offered by 10x Genomics including instrument management Access instrument information download firmware updates and monitor runs All users of each Chromium X iX instrument a...

Page 25: ...Menu Options 26 Menu Options 28 Firmware Update Procedure ...

Page 26: ...ore a complete shutdown See Moving Shipping the Instrument section Logs Access and upload past run information When filtering only those logs that appear in the filter will be uploaded to 10x Cloud downloaded to USB File capacity is 650 runs Oldest files are deleted first Log export ranges in size from 10s to 100s of MB depending on how many runs are selected To review run logs with 10x Genomics T...

Page 27: ...s own network VLAN with Internet only access System The system runs on a Linux platform The instrument s hardware and software have been securely implemented to prevent modification and to only execute trusted 10x code There is no end user configuration or access to the internal hardware OS or applications Locate information about this instrument such as serial number instrument ID and firmware ve...

Page 28: ...ats or use the one provided in the accessory kit c Insert USB into the back of the instrument d Onscreen navigate through the menu to System Firmware Update Update via USB e Click on Firmware update available f Click to download the update to the instrument g Click to install h After installation is complete remove USB from instrument Step 2 Reinitialize the instrument After installing an update r...

Page 29: ...Maintenance 30 System Maintenance 30 Moving Shipping the Instrument ...

Page 30: ...and distilled water applied to a slightly dampened lab towel As an added precaution it is recommended that the instrument be unplugged from the power source before beginning any cleaning process Service Electrical shock hazard Do not open the Chromium X iX There are no user serviceable parts inside Refer all servicing to qualified 10x Genomics service personnel Servicing is required when the Chrom...

Page 31: ...ection after relocating the instrument Step 1 Shipping Mode a Retrieve the shipping readiness test block and a gasket b Launch Shipping Mode for safe instrument transportation Menu Options System Alternate Modes Shipping Mode c Follow the screen prompts for loading the shipping readiness test block which will instruct a soft power down d Turn off the instrument completely using the power switch on...

Page 32: ...Troubleshooting 33 Error Screens ...

Page 33: ... critical error the run will immediately abort Do not proceed with any further runs Contact support 10xgenomics com with the error code System Error Pressure Error Chip Error Run Error Temperature Error Firmware Error User Recoverable Errors Follow error handling instructions through the touchscreen and continue the run Gasket Error Tray Error Chip Error Unsupported Chip Error Network Error Update...

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