Zymo Research D4200-1-100 Скачать руководство пользователя страница 4 | Manualshive

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Zymo Research D4200-1-100 Скачать руководство пользователя страница 4

ZYMO RESEARCH CORP.

Phone: (949) 679-

1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com

Page 3

Procedure Overview:

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Содержание D4200-1-100

Страница 1: ...fication of up to 100 g of transfection grade plasmid DNA using a spin column Innovative ZymoPURE technology enables elution of ultra pure endotoxin free plasmid DNA in as little as 25 l Contents Prod...

Страница 2: ...20 minutes and mix by inversion Do not microwave 4 ZymoPURE Wash 2 included with D4208S is supplied ready to use and does not require the addition of ethanol prior to use ZymoPURE Wash 2 included wit...

Страница 3: ...ed to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplificatio...

Страница 4: ...ZYMO RESEARCH CORP Phone 949 679 1190 Toll Free 888 882 9682 Fax 949 266 9452 info zymoresearch com www zymoresearch com Page 3 Procedure Overview...

Страница 5: ...y lysed when the solution appears clear purple and viscous 4 Add 250 l of ice cold ZymoPURE P3 Yellow and mix thoroughly by inversion Do not vortex Invert the tube an additional 3 4 times after the sa...

Страница 6: ...through the column 13 With the vacuum off add 200 l of ZymoPURE Wash 2 to the Zymo Spin II P Column Turn on the vacuum until all of the liquid has passed completely through the column 14 Place the Zy...

Страница 7: ...oPURE Wash 2 to the Zymo Spin II P Column and centrifuge at 5 000 x g for 1 min Discard the flow through 14 Centrifuge the Zymo Spin II P Column at 10 000 x g for 1 minute in order to remove any resid...

Страница 8: ...l of supernatant was recovered after pelleting the lysate debris For optimal performance add 275 l of ZymoPURE Binding Buffer to 600 l of clarified lysate ZymoPURE P2 and ZymoPURE Binding Buffer preci...

Страница 9: ...l 210 ml D4200 1 3 D4200 1 13 D4200 1 100 D4200 1 210 ZymoPURE P2 Green 3 ml 13 ml 100 ml 210 ml D4200 2 3 D4200 2 13 D4200 2 100 D4200 2 210 ZymoPURE P3 Yellow 3 ml 13 ml 100 ml 210 ml D4200 3 3 D420...

Страница 10: ...ZYMO RESEARCH CORP Phone 949 679 1190 Toll Free 888 882 9682 Fax 949 266 9452 info zymoresearch com www zymoresearch com Page 9...

Страница 11: ...ZYMO RESEARCH CORP Phone 949 679 1190 Toll Free 888 882 9682 Fax 949 266 9452 info zymoresearch com www zymoresearch com Page 10...

Страница 12: ...ZYMO RESEARCH CORP Phone 949 679 1190 Toll Free 888 882 9682 Fax 949 266 9452 info zymoresearch com www zymoresearch com...

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