Zeiss LSM 880, Руководство по эксплуатации

Страница: 623 / 690

CHAPTER 4 - ANNEX
LSM 880 Level-adjustable Sample Holder for LSM ZEISS
10/2014 V_01 000000-2071-464 21
9 Level-adjustable Sample Holder for LSM
Caution! Adjustable stage insert screws may intersect with parts of the microscope, e.g. the
condenser unit. When moving a motorized stage, avoid any collisions. Do not install inserts
while the stage is moving. Be careful when switching the condenser front lens.
9.1 Stage Leveling Using LSM in Reflection Mode and Focus Drive
Fig. 9
Adjustable sample holder – schematic
view
Fig. 10 Focus control with focus drive. Course of
reflection on tilted (A) and even (B)
coverslip when moving focus upwards
(left to right)
0. Adjust the edges of the clefts viewed from the right side (Fig. 9/ csv ) and back side (Fig. 9/ cbv ) to be
parallel by using screws sy and sx , respectively (Fig. 9). The cleft’s width should be approximately
2 mm as preset by the factory, but can be adjusted with screw
sz
if needed.
1. First locate the area of interest of your sample. It is important to level the sample in the same region
as the actual measurements, as the coverslip may be bent or tilted at other locations.
2. Prepare a LSM reflection image with the pinhole diameter to 1 AU.