Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
10/2014 V_01 000000-2071-464 529
6.20.2 Determination of G-factor
In order to determine the G-factor for the LSM 710 and LSM 780 systems, you need an isotropic medium
like a fluorescent dye at a 1 mM concentration, for example fluorescein. Use the multi track configuration
with P and S analyzers selected and with a beam path and channel settings appropriate to your dye. Take
a single image and activate in the
Channel
tab the
Ratio
track. In the
Ratio
panel select
Ratio Type 1
.
Set all editable summands to 0 and all factors to 1 (see Fig. 747).
For an inverted microscope, use for convenience a glass bottom dish and pipette enough solution in to
cover the glass well. For an upright stand, squeeze a drop of solution between the glass bottom dish and
a cover slip.
If you have chosen
P in the Notch filter cascade for Track 1 and assigned it to Ch1-T1 and S for track 2
assigned to Ch1-T2 then the G factor is the mean intensity value of the R1 channel. For display activate
the
Histo
register of the Image and select the R1 channel (see Fig. 748).
Fig. 747 Channels tool with Ratio
Type 1 formula for the
Ratio1 channel selected
Fig. 748 Histogram View with Ratio1 channel
activated
The G-factor is therefore defined as:
S
P
I
I
T Ch
T Ch
G
=
−
−
=
2 1
1 1