Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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DFT Fusion
(Discrete Fourier Transform Fusion): The Fusion is performed based upon the published
method ("Multiple imaging axis microscopy improves resolution for thick-sample applications", Jim
Swoger, Jan Huisken, and Ernst H. K. Stelzer, OPTICS LETTERS / Vol. 28, No. 18 / September 15, 2003).
Fine structures and details are more visible after fusion, which might be worsened when using Mean
Fusion. However, this process can create noticeable artifacts in the resulting image.
Maximum Fusion: The intensity of each pixel of both illumination images is compared and the value
with the higher intensity is used for the resulting image.
For
Mean Fusion
and
Maximum Fusion
the checkbox
Fusion Subset in X with Preview
can be
activated (Fig. 526). With these controls it is possible to define which area of the left and the right
illumination image should be used for fusion.
When this function is chosen, the image is displayed in the
Preview Tab
in the image container
(Fig. 527). In this preview mode the left hand image shows the original dataset and the right hand image
the preview of processing. Using the
dimension sliders within the image control area the Z-Position ,
Illumination
side and
View
can be chosen.
In Fusion Subset in X with Preview the currently chosen View is displayed in the Current View box.
With the Left slider or input box the area of the left illumination side is chosen. This is marked at the
slider itself with a red line, the input box shows the pixel value along the y axis. Furthermore the cut off is
shown in the preview image with a red line for easy orientation. It is best to set the
illumination slider
of the
image control area
to 1, showing the left side illumination of the original data.
Repeat the process with the Right slider and input box; here the marking lines are in blue and the
illumination slider
in the
image control area
should be placed to 2, showing the right side
illumination of the original data.
The
Preview
in the image container will show the position of both sliders
Left
and
Right
as red and blue
lines respectively (Fig. 527).
To avoid a visible change in intensity along the two cut off lines, for
Mean Fusion with Fusion Subset
in X
, an overlap of both illumination sides should remain in the center of the image and the
Blending
slider should be used to define the number of pixels used within this overlap to crossfade both
illuminations sides.
The position of the sliders
Left
and
Right
as well as the
Blending
value must be defined for each view
individually.
Fig. 526 Dual Side Fusion – Fusion Subset in X