Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
368 000000-2071-464 10/2014 V_01
5.4.19.2.2 Registration Options
The
Registration Options
check-box next to
Multiview Processing opens the interface to
define the settings for the Registration
Parameters , the Input settings , and the Fusion
options for the data (Fig. 521). Each interface is
accessed by pressing the button next to it.
The following datasets can be processed with this
task:
−
Multiview datasets, single side illumination
−
Multiview datasets, dual side illumination,
Dual Side without Fusion
−
Multiview datasets, dual side illumination,
Dual Side with Fusion
−
Multiview datasets, with Deconvolution
−
Multiview datasets, without Deconvolution
The first step is to select the
Registration Options
using the drop-down menu. The choice will
influence the
Parameters interface.
The
Registration
will generate the transformation
matrices so that all views are matched to overlay.
As a result, a dataset is provided in which all Z-
Stacks of all views should already largely overlay.
The reference at which registrations starts is always
View 1 of the dataset. This image can be used as a
control. If one or more views do not have the same
alignment, registration was not successful and the
parameters must be adjusted.
When
Apply
is pressed and the registration process has finished, the transformation matrices are saved
as an .xml file (Filename.czi.xml). Only the transformation matrix is saved if Apply is pressed and None is
selected under
Fusion
(see below). This .xml file can be used for the
Use Registration From File
option.
The Registration must always be done before the Fusion of the data.
Registration Options
are:
−
Landmark Alignment
−
Intensity Based Alignment
−
Use Registration From File
−
Interactive Registration
Fig. 521 Processing – Lightsheet Processing.
Multiview Processing