Zeiss LSM 880, Руководство по эксплуатации

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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In the
Illumination
method number of frames is
automatically set to 3 (Fig. 132).
The Illumination method changes the laser power
(Fig. 133) to acquire a series of varying exposures.
The degree of variation is fixed to allow better
reproducibility of experiments.
The second image is acquired with 3 times more
laser power and the third image is acquired with
10 x more laser power. The default laser power set
in the channel menu should be kept to a
minimum.
For the acquisition step with increased laser power,
those regions of the image are excluded from
exposures when no reasonable data can be
obtained with any higher laser power.
This mask reduces unwanted bleaching of the
sample. The respective ROIs are displayed in an
additional image. The
Illumination
method
preserves a linear grey stepping which allows for
quantitative measurements of image intensity.
Please see also the information about HDR
processing in section 5.4.12.
HDR imaging does not produce usable
results with high speed imaging.
5.2.8.6 Scan Area Definition
The
Scan area
, when expanded, displays the
Image size and Pixel Size in
µ
m of the displayed
frame (Fig. 134).
In all widefield (
WF, SIM, LSM WF
)
imaging
modes, the full frame is displayed, which cannot
be altered in size. In the
LSM imaging mode,
altering the size (
Zoom
) is possible.
If images are cropped, the size of the cropped
area and its offset from the center will be
displayed.
The horizontal and vertical sliders allow to offset a cropped region in x and y.
You can also use the graphical display to drag and drop the displayed region by pressing with the left
mouse button, which will automatically update the slider positions.
By pressing the
c buttons, the region will be centered again.
You can also type in numbers or use the arrow heads.
The frame can be altered in size by clicking at the blue anchor points, which will update the cropped area
in the next image.
For the
LSM imaging
mode, also rotation of the cropped region is possible.
Pressing
Reset
will center and expand the region to the full size.
Fig. 132 HDR Illumination mode
Fig. 133 Laser Power setting for Illumination
mode
Fig. 134 Scan Area with cropped frame offset