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ACQUIRE A MOSAIC IMAGE
1.
Proceed in the same manner as in the
ACQUIRE ONE OR SEVERAL STAININGS
section and adjust the parameters in the
brightest plane.
2.
Tick TILE SCAN to make the toolbox appears
in the MULTIDIMENTIONAL ACQUISITION
menu.
3.
There are several options in TILE SCAN
mode. Go to CENTERED GRID if you want
that your current position is considered as
the center of your mosaic.
4.
Choose the number of lines and columns.
5.
Let the OVERLAP at 10%.
6.
Tick BI-DIRECTIONAL to acquire faster.
7.
Tick ONLINE STICHING and let the
THRESHOLD at 0.10. Except if you make time
lapse tile scan imaging or multiple positions
tilescan imaging.
8.
You can have an overview of your mosaic by
clicking on SCAN OVERVIEW IMAGE. You can
choose an objective and a zoom different
from the final acquisition (be careful when
you change objectives from dry to
immersion or the contrary).
9.
Click on START EXPERIMENT to start the acquisition.
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