Zeiss Lightsheet Z.1, Руководство по эксплуатации

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CHAPTER 4 - SYSTEM OPERATION
Lightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss
02/2013 000000-1790-528 127
The
Run Batch
button will turn into a
Stop Batch
button (Fig. 192) once pressed. If the button is
pressed, batch processing will be stopped, no
matter if
Apply
was pressed or not and the button
turns back to Run Batch .
In batch processing all images can only be
processed with one parameter setting. If
images, however, have been processed
already once and are linked to processing
parameters by pressing the
Attach button,
those settings will be taken and the general
setting overruled.
If a Z-Stack was acquired you have the option to
tell the system how the frames of the stack should
be processed from the
Stack processing mode
drop down box (Fig. 193).
You have the option between
First-Last and
Single
.
When First-Last is selected the double-slider or
the left and right spin input boxes can be used to
define the first and last frame of the stack that
should be processed (Fig. 194).
When
Single is selected, the slider or spin input
box can be used to define one frame in the stack
that should be processed (Fig. 195).
With the
Mode drop down menu you have the
option to select, how the single frames of a stack
are processed (Fig. 196). You have the option
between
2D , 3D and 3D large .
Note that
3D
and
3D large
are only
available, when the image to be processed
contains a stack of >1 and
First-Last was
selected as the Stack processing mode.
Otherwise, the Mode drop down menu
shows only
2D
and is greyed.
When
2D is selected, every frame is reconstructed
with the information of that frame only and the
structuring in the thirds dimension is not used.
If
3D is selected, the information of the neighboring frames in regard to structuring information and out
of focus contribution will be used in addition to the information of the frame itself. This will result in
better x, y and z resolution and better contrast by removing the out of focus light to a greater extent at
the cost of more computational power requirement.
Fig. 192 Structured Illumination – Batch
processing
Fig. 193 Stack processing mode drop down box
Fig. 194 Setting of the first and last frame of a
stack to be processed
Fig. 195 Selection of one frame from a stack be
processed
Fig. 196 Mode drop down box