Zeiss Lightsheet Z.1, Руководство по эксплуатации

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CHAPTER 4 - SYSTEM OPERATION
Carl Zeiss Left Tool Area and Hardware Control Tools Lightsheet Z.1
92 000000-1790-528 02/2013
The effect of the filter increases with the matrix
size. This matrix / kernel size can be set for each
dimension with the three sliders
Kernel size X ,
Kernel size Y
, and
Kernel size Z
in the
Filter
Type panel or by setting the size in the spin-box
on the right hand side of each slider (Fig. 135). A
larger kernel, however, also increases the
processing time. Other settings of this filter cannot
be modified.
Smooth Filter
The smooth filter is a low pass filter with Gaussian
characteristics. The gray value of each center pixel
is replaced with the weighted average value of the
surrounding neighbor pixels. The "neighborhood"
is limited by the cut-off in pixels, set by the
Strength slider. It has an analogous effect as the
kernel size (Fig. 136).
If a dimension should be processed by this tool the
respective
X
,
Y
,
Z
, and
Channels
check boxes
must be ticked. The modified pixel now is the
center pixel of the filter matrix.
Image noise will be reduced by the application of
the low pass filter. Sharp edges of regions will blur.
Local maxima will be flattened. The dynamic range
will be reduced considerably.
Sharpen Filter
With the sharpen filter, the original image is
filtered with a low pass filter first. The result of this
filtering is then subtracted from the original image.
This will improve image sharpness.
The "neighborhood" is limited by the cut-off in
pixels, set by the
Strength slider. It has an
analogous effect as the kernel size (Fig. 137). If a
dimension should be processed by this tool, the
respective
X , Y , Z , and Channels check boxes
must be ticked.
Fig. 135 Median filter
Fig. 136 Smooth filter
Fig. 137 Sharpen filter