Zeiss Lightsheet Z.1, Руководство по эксплуатации

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CHAPTER 1 - HARDWARE
Carl Zeiss User Interfaces Lightsheet Z.1
20 000000-1790-528 02/2013
Set focus for Cam 1 (Channel 1)
•
Use the
Light Path
of the
Manual Detector Alignment
to first activate channel 1 (Cam1) and press
afterwards the Continuous button. The resulting live image shows the grating on channel 1 (Cam1).
•
Use the
Zoom
tools of the Manual Detector Alignment to zoom into the center region of the image.
•
With the live image running, open the
front system door
(Fig. 14/
1
) to the
reflector turret for
emission filter selection . This system door has no safety interlock, and opening it will not terminate
image acquisition.
•
Turn the screw which is above the turret (Fig. 14/ 3 ) with the long Allen wrench (Fig. 14/ 2 ).
•
Monitor the live image of the grating while you turn the screw in any direction and find the position
of the screw that results in the sharpest image.
•
Stop the continuous scan and close the front system door .
Set focus for Cam 2 (Channel 2)
•
Use the
Light Path
of the
Manual Detector Alignment
to direct light towards channel 2 and
activate channel 2 (Cam2).
•
Press afterwards the Continuous button. The resulting live image shows the grating on channel 2
(Cam2).
•
Use the Zoom tools for the Manual Detector Alignment to zoom into the center region of the image.
•
While the imaging continues remove the black screw cap (Fig. 15/ 1 ) on the back of the main system
module Lightsheet Z.1. This opening gives way to a screw that can be turned with an Allen wrench
(Fig. 15/
2 ).
1 Front system door
2 Allen wrench
3 Screw
Fig. 14 Position of the screw to adjust the focus of the grating for Cam 1 (channel 1)