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Switch on Procedure
1)
Switch on the 13A mains socket labelled HXP-120V. Then switch on the
epi-fluorescence HXP-120V short-arc mercury lamp power module
2)
Switch on all the other 13A mains socket switches. All the microscope
control boxes should come on (always leave the Zeiss modules in the
switched on position). The Yokogawa spinning head will also come on
(the key is left in the on position). The Evolve camera should be left off
3)
Switch on all the lasers using the remote control box (4 black switches)
4)
Switch the HP workstation PC on, and the monitor
5)
Log into the PC as Zeiss user (left option, no password)
6)
When you get into the Windows 7 desktop, switch on the large Evolve
Delta 512x512 EMCCD camera
7)
Start Zen Blue
8)
Select Zen System (Start System)
9)
Place the specimen onto the microscope
10)
Use the DAPI, GFP, RFP and BF (bright field transmission) and All-Off to
select dichroics for viewing down the microscope eyepieces (under
‘Locate’ in Zen Blue).
11)
Go to Zen Blue’s Acquisition to capture images
The microscope stand must not be left pushed backwards on the microscope or the
lasers will not fire (there is a safety cut-out)
12)
Use the GFP/DsRed dual emission filter for faster red/green scanning
13)
For complex operations like scanning multiple positions with the
motorised stage or time-lapse, press the ‘Start Experiment’ button
under Acquisition
14)
The microscope will produce better images with less photo-bleaching if
the room light is left off
