Zeiss Axioskop 2 plus, Руководство по эксплуатации

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OPERATION
Axioskop 2 plus
Carl Zeiss Illumination and contrasting techniques Axioskop 2 mot plus
3-40
B 40-075 e 02/01
•
Switch on the HBO 103 or HBO 50 mercury
vapor short-arc lamp (3-27/
2 ) and allow it to
heat up to its operation temperature for
approx. 15 mins.
•
Select the FL P&C reflector module in reflector
turret (3-27/
1 ) containing the required
fluorescence filter combination (depending on
the excitation) and switch it on.
•
When using the incident-light filter slider
(3-27/ 4 ), unblock the light path in the incident-
light illuminator.
•
Remove one eyepiece from the tube and set the
aperture diaphragm (3-27/
3 ) by opening it until
the entire objective exit pupil is unblocked.
•
Insert eyepiece in tube again and close
luminous-field diaphragm (3-27/
5 ) until it is
visible in the field of view.
•
Use the two centering screws (3-27/ 6 ) to center
the luminous-field diaphragm in relation to the
edge of the field of view.
•
Either open the luminous-field diaphragm until it just disappears behind the edge of the field of view
or, if there is a risk of specimen bleaching, reduce it until it is visible in the field of view.
•
Finally, refocus on the specimen and optimize the HBO 103 collector position as described in section
2.1.11. Set the collector in such a way that homogeneous illumination is obtained with the short-wave
excitation reflector module. When modules with long-wave excitation are used, correction of the
collector position is not required.
1 0
2
0
30
40
50
6 0
7
0
80
90
10 0
Fig. 3-27 Components for Epi-fluorescence
on the Axioskop 2
plus